Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybB and hybC overlap by 4 nucleotides hybC and hybD overlap by 1 nucleotides hybD and hybE overlap by 8 nucleotides hybE and hypA overlap by 8 nucleotides
NIAGMN_16780: hybB - Ni/Fe-hydrogenase cytochrome b subunit, at 3,317,212 to 3,318,390
hybB
NIAGMN_16785: hybC - hydrogenase 2 large subunit, at 3,318,387 to 3,320,090
hybC
NIAGMN_16790: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,320,090 to 3,320,584
hybD
NIAGMN_16795: hybE - hydrogenase-2 assembly chaperone, at 3,320,577 to 3,321,065
hybE
NIAGMN_16800: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,321,058 to 3,321,399
hypA
Position (kb) 3318
3319
3320
3321 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 3317.438 kb on + strand, within hybB at 3317.438 kb on + strand, within hybB at 3317.438 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.440 kb on + strand, within hybB at 3317.441 kb on - strand, within hybB at 3317.441 kb on - strand, within hybB at 3317.441 kb on - strand, within hybB at 3317.441 kb on - strand, within hybB at 3317.441 kb on - strand, within hybB at 3317.449 kb on - strand, within hybB at 3317.461 kb on + strand, within hybB at 3317.462 kb on - strand, within hybB at 3317.549 kb on - strand, within hybB at 3317.564 kb on - strand, within hybB at 3317.569 kb on + strand, within hybB at 3317.622 kb on + strand, within hybB at 3317.623 kb on - strand, within hybB at 3317.632 kb on + strand, within hybB at 3317.632 kb on + strand, within hybB at 3317.632 kb on + strand, within hybB at 3317.632 kb on + strand, within hybB at 3317.632 kb on + strand, within hybB at 3317.633 kb on - strand, within hybB at 3317.633 kb on - strand, within hybB at 3317.700 kb on - strand, within hybB at 3317.801 kb on - strand, within hybB at 3317.801 kb on - strand, within hybB at 3317.827 kb on + strand, within hybB at 3317.827 kb on + strand, within hybB at 3318.018 kb on + strand, within hybB at 3318.018 kb on + strand, within hybB at 3318.018 kb on + strand, within hybB at 3318.018 kb on + strand, within hybB at 3318.019 kb on - strand, within hybB at 3318.019 kb on - strand, within hybB at 3318.179 kb on + strand, within hybB at 3318.179 kb on + strand, within hybB at 3318.179 kb on + strand, within hybB at 3318.180 kb on - strand, within hybB at 3318.205 kb on + strand, within hybB at 3318.205 kb on + strand, within hybB at 3318.245 kb on - strand, within hybB at 3318.245 kb on - strand, within hybB at 3318.307 kb on + strand at 3318.307 kb on + strand at 3318.307 kb on + strand at 3318.308 kb on - strand at 3318.308 kb on - strand at 3318.315 kb on - strand at 3318.336 kb on - strand at 3318.336 kb on - strand at 3318.363 kb on + strand at 3318.401 kb on + strand at 3318.401 kb on + strand at 3318.401 kb on + strand at 3318.401 kb on + strand at 3318.401 kb on + strand at 3318.402 kb on - strand at 3318.402 kb on - strand at 3318.402 kb on - strand at 3318.405 kb on - strand at 3318.405 kb on - strand at 3318.405 kb on - strand at 3318.436 kb on + strand at 3318.437 kb on - strand at 3318.437 kb on - strand at 3318.563 kb on + strand, within hybC at 3318.563 kb on + strand, within hybC at 3318.564 kb on - strand, within hybC at 3318.564 kb on - strand, within hybC at 3318.564 kb on - strand, within hybC at 3318.574 kb on + strand, within hybC at 3318.574 kb on + strand, within hybC at 3318.574 kb on + strand, within hybC at 3318.575 kb on - strand, within hybC at 3318.575 kb on - strand, within hybC at 3318.575 kb on - strand, within hybC at 3318.575 kb on - strand, within hybC at 3318.578 kb on + strand, within hybC at 3318.578 kb on + strand, within hybC at 3318.578 kb on + strand, within hybC at 3318.578 kb on + strand, within hybC at 3318.578 kb on + strand, within hybC at 3318.579 kb on - strand, within hybC at 3318.581 kb on + strand, within hybC at 3318.582 kb on - strand, within hybC at 3318.582 kb on - strand, within hybC at 3318.582 kb on - strand, within hybC at 3318.582 kb on - strand, within hybC at 3318.654 kb on + strand, within hybC at 3318.654 kb on + strand, within hybC at 3318.654 kb on + strand, within hybC at 3318.655 kb on - strand, within hybC at 3318.662 kb on + strand, within hybC at 3318.701 kb on - strand, within hybC at 3318.711 kb on + strand, within hybC at 3318.882 kb on - strand, within hybC at 3318.957 kb on + strand, within hybC at 3319.045 kb on + strand, within hybC at 3319.046 kb on - strand, within hybC at 3319.046 kb on - strand, within hybC at 3319.110 kb on + strand, within hybC at 3319.110 kb on + strand, within hybC at 3319.110 kb on + strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.111 kb on - strand, within hybC at 3319.159 kb on - strand, within hybC at 3319.159 kb on - strand, within hybC at 3319.169 kb on + strand, within hybC at 3319.170 kb on - strand, within hybC at 3319.230 kb on + strand, within hybC at 3319.230 kb on + strand, within hybC at 3319.231 kb on - strand, within hybC at 3319.231 kb on - strand, within hybC at 3319.263 kb on - strand, within hybC at 3319.293 kb on + strand, within hybC at 3319.293 kb on + strand, within hybC at 3319.294 kb on - strand, within hybC at 3319.320 kb on + strand, within hybC at 3319.321 kb on - strand, within hybC at 3319.350 kb on + strand, within hybC at 3319.350 kb on + strand, within hybC at 3319.350 kb on + strand, within hybC at 3319.351 kb on - strand, within hybC at 3319.392 kb on + strand, within hybC at 3319.393 kb on - strand, within hybC at 3319.394 kb on + strand, within hybC at 3319.394 kb on + strand, within hybC at 3319.395 kb on - strand, within hybC at 3319.395 kb on - strand, within hybC at 3319.427 kb on + strand, within hybC at 3319.443 kb on + strand, within hybC at 3319.444 kb on - strand, within hybC at 3319.470 kb on + strand, within hybC at 3319.470 kb on + strand, within hybC at 3319.530 kb on - strand, within hybC at 3319.532 kb on + strand, within hybC at 3319.532 kb on + strand, within hybC at 3319.532 kb on + strand, within hybC at 3319.532 kb on + strand, within hybC at 3319.532 kb on + strand, within hybC at 3319.533 kb on - strand, within hybC at 3319.713 kb on + strand, within hybC at 3319.714 kb on - strand, within hybC at 3319.826 kb on + strand, within hybC at 3319.827 kb on - strand, within hybC at 3319.872 kb on + strand, within hybC at 3319.872 kb on + strand, within hybC at 3319.872 kb on + strand, within hybC at 3319.873 kb on - strand, within hybC at 3319.938 kb on + strand at 3319.959 kb on - strand at 3319.959 kb on - strand at 3320.000 kb on + strand at 3320.000 kb on + strand at 3320.001 kb on - strand at 3320.038 kb on + strand at 3320.044 kb on + strand at 3320.044 kb on + strand at 3320.045 kb on - strand at 3320.045 kb on - strand at 3320.089 kb on - strand at 3320.095 kb on + strand at 3320.095 kb on + strand at 3320.100 kb on + strand at 3320.119 kb on + strand at 3320.167 kb on - strand, within hybD at 3320.177 kb on + strand, within hybD at 3320.177 kb on + strand, within hybD at 3320.192 kb on + strand, within hybD at 3320.192 kb on + strand, within hybD at 3320.192 kb on + strand, within hybD at 3320.192 kb on + strand, within hybD at 3320.347 kb on + strand, within hybD at 3320.445 kb on + strand, within hybD at 3320.590 kb on + strand at 3320.591 kb on - strand at 3320.623 kb on + strand at 3320.697 kb on + strand, within hybE at 3320.697 kb on + strand, within hybE at 3320.698 kb on - strand, within hybE at 3320.712 kb on - strand, within hybE at 3320.890 kb on - strand, within hybE at 3320.890 kb on - strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.927 kb on + strand, within hybE at 3320.928 kb on - strand, within hybE at 3320.928 kb on - strand, within hybE at 3321.007 kb on + strand, within hybE at 3321.007 kb on + strand, within hybE at 3321.007 kb on + strand, within hybE at 3321.008 kb on - strand, within hybE at 3321.008 kb on - strand, within hybE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,317,438 + hybB NIAGMN_16780 0.19 +0.5 3,317,438 + hybB NIAGMN_16780 0.19 +1.5 3,317,438 + hybB NIAGMN_16780 0.19 +0.4 3,317,440 + hybB NIAGMN_16780 0.19 -0.2 3,317,440 + hybB NIAGMN_16780 0.19 +0.4 3,317,440 + hybB NIAGMN_16780 0.19 -0.9 3,317,440 + hybB NIAGMN_16780 0.19 +1.4 3,317,440 + hybB NIAGMN_16780 0.19 +0.5 3,317,440 + hybB NIAGMN_16780 0.19 -0.0 3,317,441 - hybB NIAGMN_16780 0.19 -2.4 3,317,441 - hybB NIAGMN_16780 0.19 -1.1 3,317,441 - hybB NIAGMN_16780 0.19 +0.6 3,317,441 - hybB NIAGMN_16780 0.19 +0.9 3,317,441 - hybB NIAGMN_16780 0.19 -0.4 3,317,449 - hybB NIAGMN_16780 0.20 +0.8 3,317,461 + hybB NIAGMN_16780 0.21 -0.0 3,317,462 - hybB NIAGMN_16780 0.21 -0.2 3,317,549 - hybB NIAGMN_16780 0.29 +2.8 3,317,564 - hybB NIAGMN_16780 0.30 -0.4 3,317,569 + hybB NIAGMN_16780 0.30 -3.7 3,317,622 + hybB NIAGMN_16780 0.35 +1.9 3,317,623 - hybB NIAGMN_16780 0.35 -2.4 3,317,632 + hybB NIAGMN_16780 0.36 +0.1 3,317,632 + hybB NIAGMN_16780 0.36 -0.9 3,317,632 + hybB NIAGMN_16780 0.36 -1.8 3,317,632 + hybB NIAGMN_16780 0.36 -1.4 3,317,632 + hybB NIAGMN_16780 0.36 +1.7 3,317,633 - hybB NIAGMN_16780 0.36 -3.0 3,317,633 - hybB NIAGMN_16780 0.36 +1.4 3,317,700 - hybB NIAGMN_16780 0.41 -1.7 3,317,801 - hybB NIAGMN_16780 0.50 -0.7 3,317,801 - hybB NIAGMN_16780 0.50 -1.6 3,317,827 + hybB NIAGMN_16780 0.52 -0.1 3,317,827 + hybB NIAGMN_16780 0.52 -1.7 3,318,018 + hybB NIAGMN_16780 0.68 +0.9 3,318,018 + hybB NIAGMN_16780 0.68 +0.0 3,318,018 + hybB NIAGMN_16780 0.68 -1.3 3,318,018 + hybB NIAGMN_16780 0.68 +0.0 3,318,019 - hybB NIAGMN_16780 0.68 +0.9 3,318,019 - hybB NIAGMN_16780 0.68 +2.2 3,318,179 + hybB NIAGMN_16780 0.82 +0.1 3,318,179 + hybB NIAGMN_16780 0.82 +2.0 3,318,179 + hybB NIAGMN_16780 0.82 -0.9 3,318,180 - hybB NIAGMN_16780 0.82 +2.0 3,318,205 + hybB NIAGMN_16780 0.84 -0.2 3,318,205 + hybB NIAGMN_16780 0.84 +0.5 3,318,245 - hybB NIAGMN_16780 0.88 -0.0 3,318,245 - hybB NIAGMN_16780 0.88 -1.5 3,318,307 + -1.4 3,318,307 + +2.6 3,318,307 + -0.8 3,318,308 - +2.7 3,318,308 - +0.3 3,318,315 - +0.2 3,318,336 - -1.4 3,318,336 - -0.8 3,318,363 + +0.4 3,318,401 + +0.1 3,318,401 + -0.2 3,318,401 + -3.0 3,318,401 + +0.9 3,318,401 + -1.6 3,318,402 - -1.3 3,318,402 - +0.5 3,318,402 - +1.4 3,318,405 - +1.4 3,318,405 - -3.1 3,318,405 - +0.1 3,318,436 + +1.6 3,318,437 - +0.4 3,318,437 - +0.4 3,318,563 + hybC NIAGMN_16785 0.10 -1.5 3,318,563 + hybC NIAGMN_16785 0.10 +1.6 3,318,564 - hybC NIAGMN_16785 0.10 +0.0 3,318,564 - hybC NIAGMN_16785 0.10 -0.3 3,318,564 - hybC NIAGMN_16785 0.10 +0.1 3,318,574 + hybC NIAGMN_16785 0.11 -2.1 3,318,574 + hybC NIAGMN_16785 0.11 -0.3 3,318,574 + hybC NIAGMN_16785 0.11 -0.2 3,318,575 - hybC NIAGMN_16785 0.11 +0.4 3,318,575 - hybC NIAGMN_16785 0.11 -1.6 3,318,575 - hybC NIAGMN_16785 0.11 -2.9 3,318,575 - hybC NIAGMN_16785 0.11 -0.1 3,318,578 + hybC NIAGMN_16785 0.11 -2.1 3,318,578 + hybC NIAGMN_16785 0.11 -2.5 3,318,578 + hybC NIAGMN_16785 0.11 +1.0 3,318,578 + hybC NIAGMN_16785 0.11 -0.2 3,318,578 + hybC NIAGMN_16785 0.11 +1.4 3,318,579 - hybC NIAGMN_16785 0.11 -2.2 3,318,581 + hybC NIAGMN_16785 0.11 -0.2 3,318,582 - hybC NIAGMN_16785 0.11 +0.1 3,318,582 - hybC NIAGMN_16785 0.11 -1.1 3,318,582 - hybC NIAGMN_16785 0.11 +0.5 3,318,582 - hybC NIAGMN_16785 0.11 -1.1 3,318,654 + hybC NIAGMN_16785 0.16 -0.9 3,318,654 + hybC NIAGMN_16785 0.16 -1.5 3,318,654 + hybC NIAGMN_16785 0.16 +2.0 3,318,655 - hybC NIAGMN_16785 0.16 +1.6 3,318,662 + hybC NIAGMN_16785 0.16 +1.9 3,318,701 - hybC NIAGMN_16785 0.18 -1.0 3,318,711 + hybC NIAGMN_16785 0.19 +1.7 3,318,882 - hybC NIAGMN_16785 0.29 -1.0 3,318,957 + hybC NIAGMN_16785 0.33 -0.1 3,319,045 + hybC NIAGMN_16785 0.39 -1.3 3,319,046 - hybC NIAGMN_16785 0.39 -0.5 3,319,046 - hybC NIAGMN_16785 0.39 +0.2 3,319,110 + hybC NIAGMN_16785 0.42 +0.2 3,319,110 + hybC NIAGMN_16785 0.42 +0.7 3,319,110 + hybC NIAGMN_16785 0.42 +0.1 3,319,111 - hybC NIAGMN_16785 0.42 +0.0 3,319,111 - hybC NIAGMN_16785 0.42 -1.3 3,319,111 - hybC NIAGMN_16785 0.42 -0.1 3,319,111 - hybC NIAGMN_16785 0.42 -1.3 3,319,111 - hybC NIAGMN_16785 0.42 +1.6 3,319,111 - hybC NIAGMN_16785 0.42 +0.3 3,319,111 - hybC NIAGMN_16785 0.42 -0.8 3,319,111 - hybC NIAGMN_16785 0.42 -0.6 3,319,111 - hybC NIAGMN_16785 0.42 -2.2 3,319,159 - hybC NIAGMN_16785 0.45 +1.0 3,319,159 - hybC NIAGMN_16785 0.45 +0.1 3,319,169 + hybC NIAGMN_16785 0.46 -1.3 3,319,170 - hybC NIAGMN_16785 0.46 +1.1 3,319,230 + hybC NIAGMN_16785 0.49 +1.4 3,319,230 + hybC NIAGMN_16785 0.49 +0.0 3,319,231 - hybC NIAGMN_16785 0.50 +0.6 3,319,231 - hybC NIAGMN_16785 0.50 -0.9 3,319,263 - hybC NIAGMN_16785 0.51 -2.9 3,319,293 + hybC NIAGMN_16785 0.53 +1.8 3,319,293 + hybC NIAGMN_16785 0.53 +1.5 3,319,294 - hybC NIAGMN_16785 0.53 -1.7 3,319,320 + hybC NIAGMN_16785 0.55 +0.2 3,319,321 - hybC NIAGMN_16785 0.55 -3.5 3,319,350 + hybC NIAGMN_16785 0.57 +0.4 3,319,350 + hybC NIAGMN_16785 0.57 -0.7 3,319,350 + hybC NIAGMN_16785 0.57 -1.0 3,319,351 - hybC NIAGMN_16785 0.57 -0.7 3,319,392 + hybC NIAGMN_16785 0.59 +1.1 3,319,393 - hybC NIAGMN_16785 0.59 +1.6 3,319,394 + hybC NIAGMN_16785 0.59 +0.9 3,319,394 + hybC NIAGMN_16785 0.59 -0.4 3,319,395 - hybC NIAGMN_16785 0.59 -0.9 3,319,395 - hybC NIAGMN_16785 0.59 +0.4 3,319,427 + hybC NIAGMN_16785 0.61 -2.5 3,319,443 + hybC NIAGMN_16785 0.62 +0.3 3,319,444 - hybC NIAGMN_16785 0.62 +1.2 3,319,470 + hybC NIAGMN_16785 0.64 +0.2 3,319,470 + hybC NIAGMN_16785 0.64 +0.1 3,319,530 - hybC NIAGMN_16785 0.67 -0.4 3,319,532 + hybC NIAGMN_16785 0.67 -0.6 3,319,532 + hybC NIAGMN_16785 0.67 +0.5 3,319,532 + hybC NIAGMN_16785 0.67 -1.0 3,319,532 + hybC NIAGMN_16785 0.67 +0.5 3,319,532 + hybC NIAGMN_16785 0.67 -1.4 3,319,533 - hybC NIAGMN_16785 0.67 -0.0 3,319,713 + hybC NIAGMN_16785 0.78 +0.6 3,319,714 - hybC NIAGMN_16785 0.78 -4.6 3,319,826 + hybC NIAGMN_16785 0.84 +0.2 3,319,827 - hybC NIAGMN_16785 0.85 -1.3 3,319,872 + hybC NIAGMN_16785 0.87 +1.0 3,319,872 + hybC NIAGMN_16785 0.87 +0.4 3,319,872 + hybC NIAGMN_16785 0.87 -1.1 3,319,873 - hybC NIAGMN_16785 0.87 -0.0 3,319,938 + +0.2 3,319,959 - -3.9 3,319,959 - +0.7 3,320,000 + +0.5 3,320,000 + +0.5 3,320,001 - -2.1 3,320,038 + -1.8 3,320,044 + +0.1 3,320,044 + -3.1 3,320,045 - -0.1 3,320,045 - -2.7 3,320,089 - -2.4 3,320,095 + +0.5 3,320,095 + -1.3 3,320,100 + +1.3 3,320,119 + +0.1 3,320,167 - hybD NIAGMN_16790 0.16 +2.0 3,320,177 + hybD NIAGMN_16790 0.18 -0.2 3,320,177 + hybD NIAGMN_16790 0.18 +0.2 3,320,192 + hybD NIAGMN_16790 0.21 -3.1 3,320,192 + hybD NIAGMN_16790 0.21 -0.4 3,320,192 + hybD NIAGMN_16790 0.21 +2.2 3,320,192 + hybD NIAGMN_16790 0.21 +0.5 3,320,347 + hybD NIAGMN_16790 0.52 +0.5 3,320,445 + hybD NIAGMN_16790 0.72 -0.1 3,320,590 + +0.4 3,320,591 - -0.8 3,320,623 + -0.1 3,320,697 + hybE NIAGMN_16795 0.25 +0.0 3,320,697 + hybE NIAGMN_16795 0.25 -0.1 3,320,698 - hybE NIAGMN_16795 0.25 -2.4 3,320,712 - hybE NIAGMN_16795 0.28 +0.4 3,320,890 - hybE NIAGMN_16795 0.64 -2.1 3,320,890 - hybE NIAGMN_16795 0.64 -1.3 3,320,927 + hybE NIAGMN_16795 0.72 +0.6 3,320,927 + hybE NIAGMN_16795 0.72 +1.3 3,320,927 + hybE NIAGMN_16795 0.72 -0.3 3,320,927 + hybE NIAGMN_16795 0.72 +0.4 3,320,927 + hybE NIAGMN_16795 0.72 -2.7 3,320,927 + hybE NIAGMN_16795 0.72 -0.9 3,320,927 + hybE NIAGMN_16795 0.72 -0.4 3,320,928 - hybE NIAGMN_16795 0.72 -0.4 3,320,928 - hybE NIAGMN_16795 0.72 -0.2 3,321,007 + hybE NIAGMN_16795 0.88 +0.5 3,321,007 + hybE NIAGMN_16795 0.88 -2.2 3,321,007 + hybE NIAGMN_16795 0.88 -0.2 3,321,008 - hybE NIAGMN_16795 0.88 -0.6 3,321,008 - hybE NIAGMN_16795 0.88 +1.5
Or see this region's nucleotide sequence