Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdcE and tdcF are separated by 13 nucleotides tdcF and tdcG are separated by 71 nucleotides tdcG and dlsT are separated by 274 nucleotides
NIAGMN_16180: tdcE - 2-ketobutyrate formate-lyase/pyruvate formate-lyase, at 3,199,681 to 3,201,975
tdcE
NIAGMN_16185: tdcF - enamine/imine deaminase, at 3,201,989 to 3,202,378
tdcF
NIAGMN_16190: tdcG - L-serine ammonia-lyase, at 3,202,450 to 3,203,814
tdcG
NIAGMN_16195: dlsT - putative serine transporter, at 3,204,089 to 3,205,420
dlsT
Position (kb)
3202
3203
3204 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3201.475 kb on + strand, within tdcE at 3201.520 kb on - strand, within tdcE at 3201.667 kb on + strand, within tdcE at 3201.689 kb on - strand, within tdcE at 3201.735 kb on - strand, within tdcE at 3201.735 kb on - strand, within tdcE at 3201.738 kb on + strand, within tdcE at 3201.738 kb on + strand, within tdcE at 3201.739 kb on - strand, within tdcE at 3201.739 kb on - strand, within tdcE at 3201.739 kb on - strand, within tdcE at 3201.739 kb on - strand, within tdcE at 3201.769 kb on + strand at 3201.821 kb on + strand at 3201.822 kb on - strand at 3201.822 kb on - strand at 3201.874 kb on - strand at 3201.945 kb on + strand at 3201.946 kb on - strand at 3201.954 kb on + strand at 3201.955 kb on - strand at 3201.955 kb on - strand at 3201.955 kb on - strand at 3201.989 kb on - strand at 3201.989 kb on - strand at 3201.989 kb on - strand at 3201.989 kb on - strand at 3202.037 kb on + strand, within tdcF at 3202.143 kb on + strand, within tdcF at 3202.143 kb on + strand, within tdcF at 3202.198 kb on + strand, within tdcF at 3202.259 kb on + strand, within tdcF at 3202.259 kb on + strand, within tdcF at 3202.259 kb on + strand, within tdcF at 3202.260 kb on - strand, within tdcF at 3202.260 kb on - strand, within tdcF at 3202.260 kb on - strand, within tdcF at 3202.261 kb on + strand, within tdcF at 3202.293 kb on - strand, within tdcF at 3202.311 kb on + strand, within tdcF at 3202.357 kb on + strand at 3202.365 kb on + strand at 3202.365 kb on + strand at 3202.369 kb on + strand at 3202.369 kb on + strand at 3202.369 kb on + strand at 3202.369 kb on + strand at 3202.370 kb on - strand at 3202.370 kb on - strand at 3202.370 kb on - strand at 3202.370 kb on - strand at 3202.402 kb on - strand at 3202.450 kb on - strand at 3202.456 kb on - strand at 3202.456 kb on - strand at 3202.456 kb on - strand at 3202.554 kb on + strand at 3202.562 kb on + strand at 3202.563 kb on - strand at 3202.563 kb on - strand at 3202.563 kb on - strand at 3202.581 kb on + strand at 3202.581 kb on + strand at 3202.582 kb on - strand at 3202.582 kb on - strand at 3202.597 kb on + strand, within tdcG at 3202.597 kb on + strand, within tdcG at 3202.709 kb on + strand, within tdcG at 3202.715 kb on + strand, within tdcG at 3202.715 kb on + strand, within tdcG at 3202.788 kb on + strand, within tdcG at 3202.862 kb on + strand, within tdcG at 3202.862 kb on + strand, within tdcG at 3202.862 kb on + strand, within tdcG at 3202.863 kb on - strand, within tdcG at 3202.863 kb on - strand, within tdcG at 3202.863 kb on - strand, within tdcG at 3202.865 kb on + strand, within tdcG at 3202.865 kb on + strand, within tdcG at 3202.865 kb on + strand, within tdcG at 3202.866 kb on - strand, within tdcG at 3202.866 kb on - strand, within tdcG at 3202.866 kb on - strand, within tdcG at 3202.866 kb on - strand, within tdcG at 3202.870 kb on - strand, within tdcG at 3202.876 kb on + strand, within tdcG at 3202.876 kb on + strand, within tdcG at 3202.876 kb on + strand, within tdcG at 3202.877 kb on - strand, within tdcG at 3202.877 kb on - strand, within tdcG at 3202.879 kb on + strand, within tdcG at 3202.880 kb on - strand, within tdcG at 3202.949 kb on + strand, within tdcG at 3202.954 kb on + strand, within tdcG at 3202.955 kb on - strand, within tdcG at 3202.955 kb on - strand, within tdcG at 3202.955 kb on - strand, within tdcG at 3202.955 kb on - strand, within tdcG at 3202.997 kb on - strand, within tdcG at 3202.997 kb on - strand, within tdcG at 3203.039 kb on + strand, within tdcG at 3203.040 kb on - strand, within tdcG at 3203.052 kb on + strand, within tdcG at 3203.052 kb on + strand, within tdcG at 3203.203 kb on - strand, within tdcG at 3203.248 kb on + strand, within tdcG at 3203.248 kb on + strand, within tdcG at 3203.248 kb on + strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.249 kb on - strand, within tdcG at 3203.344 kb on + strand, within tdcG at 3203.345 kb on - strand, within tdcG at 3203.347 kb on + strand, within tdcG at 3203.347 kb on + strand, within tdcG at 3203.348 kb on - strand, within tdcG at 3203.352 kb on + strand, within tdcG at 3203.352 kb on + strand, within tdcG at 3203.353 kb on - strand, within tdcG at 3203.412 kb on + strand, within tdcG at 3203.511 kb on - strand, within tdcG at 3203.519 kb on + strand, within tdcG at 3203.519 kb on + strand, within tdcG at 3203.520 kb on - strand, within tdcG at 3203.520 kb on - strand, within tdcG at 3203.520 kb on - strand, within tdcG at 3203.543 kb on + strand, within tdcG at 3203.544 kb on - strand, within tdcG at 3203.544 kb on - strand, within tdcG at 3203.574 kb on + strand, within tdcG at 3203.574 kb on + strand, within tdcG at 3203.574 kb on + strand, within tdcG at 3203.612 kb on + strand, within tdcG at 3203.634 kb on + strand, within tdcG at 3203.634 kb on + strand, within tdcG at 3203.634 kb on + strand, within tdcG at 3203.634 kb on + strand, within tdcG at 3203.715 kb on + strand at 3203.735 kb on - strand at 3203.735 kb on - strand at 3203.735 kb on - strand at 3203.751 kb on + strand at 3203.751 kb on + strand at 3203.751 kb on + strand at 3203.752 kb on - strand at 3203.760 kb on + strand at 3203.760 kb on + strand at 3203.761 kb on - strand at 3203.764 kb on + strand at 3203.881 kb on - strand at 3203.997 kb on + strand at 3203.997 kb on + strand at 3204.215 kb on - strand at 3204.215 kb on - strand at 3204.326 kb on + strand, within dlsT at 3204.326 kb on + strand, within dlsT at 3204.326 kb on + strand, within dlsT at 3204.327 kb on - strand, within dlsT at 3204.327 kb on - strand, within dlsT at 3204.327 kb on - strand, within dlsT at 3204.346 kb on + strand, within dlsT at 3204.346 kb on + strand, within dlsT at 3204.347 kb on - strand, within dlsT at 3204.349 kb on + strand, within dlsT at 3204.350 kb on - strand, within dlsT at 3204.350 kb on - strand, within dlsT at 3204.352 kb on + strand, within dlsT at 3204.352 kb on + strand, within dlsT at 3204.352 kb on + strand, within dlsT at 3204.432 kb on + strand, within dlsT at 3204.443 kb on + strand, within dlsT at 3204.443 kb on + strand, within dlsT at 3204.443 kb on + strand, within dlsT at 3204.444 kb on - strand, within dlsT at 3204.509 kb on + strand, within dlsT at 3204.510 kb on - strand, within dlsT at 3204.517 kb on + strand, within dlsT at 3204.517 kb on + strand, within dlsT at 3204.517 kb on + strand, within dlsT at 3204.517 kb on + strand, within dlsT at 3204.517 kb on + strand, within dlsT at 3204.518 kb on - strand, within dlsT at 3204.518 kb on - strand, within dlsT at 3204.518 kb on - strand, within dlsT at 3204.560 kb on + strand, within dlsT at 3204.561 kb on - strand, within dlsT at 3204.561 kb on - strand, within dlsT at 3204.704 kb on + strand, within dlsT at 3204.704 kb on + strand, within dlsT at 3204.705 kb on - strand, within dlsT at 3204.705 kb on - strand, within dlsT at 3204.705 kb on - strand, within dlsT at 3204.705 kb on - strand, within dlsT at 3204.754 kb on + strand, within dlsT at 3204.757 kb on + strand, within dlsT at 3204.757 kb on + strand, within dlsT at 3204.758 kb on - strand, within dlsT at 3204.801 kb on + strand, within dlsT at 3204.801 kb on + strand, within dlsT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,201,475 + tdcE NIAGMN_16180 0.78 +1.3 3,201,520 - tdcE NIAGMN_16180 0.80 +0.1 3,201,667 + tdcE NIAGMN_16180 0.87 +1.2 3,201,689 - tdcE NIAGMN_16180 0.87 +2.3 3,201,735 - tdcE NIAGMN_16180 0.89 +0.6 3,201,735 - tdcE NIAGMN_16180 0.89 -1.0 3,201,738 + tdcE NIAGMN_16180 0.90 -0.5 3,201,738 + tdcE NIAGMN_16180 0.90 -1.0 3,201,739 - tdcE NIAGMN_16180 0.90 -0.1 3,201,739 - tdcE NIAGMN_16180 0.90 -0.6 3,201,739 - tdcE NIAGMN_16180 0.90 +1.5 3,201,739 - tdcE NIAGMN_16180 0.90 -0.4 3,201,769 + +1.0 3,201,821 + -2.4 3,201,822 - -0.8 3,201,822 - -1.5 3,201,874 - +1.2 3,201,945 + -0.3 3,201,946 - -0.1 3,201,954 + +1.5 3,201,955 - +0.7 3,201,955 - +0.5 3,201,955 - +0.7 3,201,989 - +0.4 3,201,989 - +0.9 3,201,989 - +0.2 3,201,989 - +0.8 3,202,037 + tdcF NIAGMN_16185 0.12 -1.5 3,202,143 + tdcF NIAGMN_16185 0.39 +0.9 3,202,143 + tdcF NIAGMN_16185 0.39 +2.0 3,202,198 + tdcF NIAGMN_16185 0.54 -3.5 3,202,259 + tdcF NIAGMN_16185 0.69 -1.9 3,202,259 + tdcF NIAGMN_16185 0.69 -0.8 3,202,259 + tdcF NIAGMN_16185 0.69 +0.9 3,202,260 - tdcF NIAGMN_16185 0.69 -0.6 3,202,260 - tdcF NIAGMN_16185 0.69 +2.0 3,202,260 - tdcF NIAGMN_16185 0.69 +1.5 3,202,261 + tdcF NIAGMN_16185 0.70 -0.2 3,202,293 - tdcF NIAGMN_16185 0.78 -0.3 3,202,311 + tdcF NIAGMN_16185 0.83 -1.1 3,202,357 + -0.8 3,202,365 + -0.9 3,202,365 + +0.5 3,202,369 + +0.2 3,202,369 + -0.8 3,202,369 + +1.7 3,202,369 + +1.4 3,202,370 - -0.3 3,202,370 - -1.0 3,202,370 - +0.7 3,202,370 - -0.8 3,202,402 - -1.5 3,202,450 - -0.2 3,202,456 - +1.0 3,202,456 - -3.7 3,202,456 - +0.9 3,202,554 + +1.2 3,202,562 + +2.9 3,202,563 - +0.9 3,202,563 - +1.3 3,202,563 - +0.8 3,202,581 + -0.5 3,202,581 + +0.2 3,202,582 - -3.2 3,202,582 - +0.5 3,202,597 + tdcG NIAGMN_16190 0.11 -0.5 3,202,597 + tdcG NIAGMN_16190 0.11 -2.5 3,202,709 + tdcG NIAGMN_16190 0.19 -0.0 3,202,715 + tdcG NIAGMN_16190 0.19 -0.3 3,202,715 + tdcG NIAGMN_16190 0.19 -0.3 3,202,788 + tdcG NIAGMN_16190 0.25 +0.6 3,202,862 + tdcG NIAGMN_16190 0.30 +0.5 3,202,862 + tdcG NIAGMN_16190 0.30 -2.4 3,202,862 + tdcG NIAGMN_16190 0.30 +0.5 3,202,863 - tdcG NIAGMN_16190 0.30 -0.5 3,202,863 - tdcG NIAGMN_16190 0.30 -0.2 3,202,863 - tdcG NIAGMN_16190 0.30 -2.4 3,202,865 + tdcG NIAGMN_16190 0.30 +1.1 3,202,865 + tdcG NIAGMN_16190 0.30 -0.9 3,202,865 + tdcG NIAGMN_16190 0.30 -0.5 3,202,866 - tdcG NIAGMN_16190 0.30 +1.1 3,202,866 - tdcG NIAGMN_16190 0.30 -0.4 3,202,866 - tdcG NIAGMN_16190 0.30 +0.5 3,202,866 - tdcG NIAGMN_16190 0.30 +0.2 3,202,870 - tdcG NIAGMN_16190 0.31 -1.9 3,202,876 + tdcG NIAGMN_16190 0.31 -0.1 3,202,876 + tdcG NIAGMN_16190 0.31 +0.6 3,202,876 + tdcG NIAGMN_16190 0.31 +0.4 3,202,877 - tdcG NIAGMN_16190 0.31 +0.9 3,202,877 - tdcG NIAGMN_16190 0.31 -3.5 3,202,879 + tdcG NIAGMN_16190 0.31 +0.3 3,202,880 - tdcG NIAGMN_16190 0.32 -1.5 3,202,949 + tdcG NIAGMN_16190 0.37 -1.3 3,202,954 + tdcG NIAGMN_16190 0.37 -3.4 3,202,955 - tdcG NIAGMN_16190 0.37 -1.7 3,202,955 - tdcG NIAGMN_16190 0.37 +1.5 3,202,955 - tdcG NIAGMN_16190 0.37 +0.6 3,202,955 - tdcG NIAGMN_16190 0.37 -2.9 3,202,997 - tdcG NIAGMN_16190 0.40 -1.7 3,202,997 - tdcG NIAGMN_16190 0.40 +0.1 3,203,039 + tdcG NIAGMN_16190 0.43 -1.5 3,203,040 - tdcG NIAGMN_16190 0.43 +0.6 3,203,052 + tdcG NIAGMN_16190 0.44 -0.7 3,203,052 + tdcG NIAGMN_16190 0.44 +0.2 3,203,203 - tdcG NIAGMN_16190 0.55 -2.1 3,203,248 + tdcG NIAGMN_16190 0.58 +2.0 3,203,248 + tdcG NIAGMN_16190 0.58 -2.6 3,203,248 + tdcG NIAGMN_16190 0.58 +2.2 3,203,249 - tdcG NIAGMN_16190 0.59 -0.3 3,203,249 - tdcG NIAGMN_16190 0.59 +0.9 3,203,249 - tdcG NIAGMN_16190 0.59 -0.2 3,203,249 - tdcG NIAGMN_16190 0.59 -1.1 3,203,249 - tdcG NIAGMN_16190 0.59 +0.5 3,203,249 - tdcG NIAGMN_16190 0.59 +0.0 3,203,344 + tdcG NIAGMN_16190 0.65 -3.3 3,203,345 - tdcG NIAGMN_16190 0.66 +1.1 3,203,347 + tdcG NIAGMN_16190 0.66 +0.6 3,203,347 + tdcG NIAGMN_16190 0.66 +0.5 3,203,348 - tdcG NIAGMN_16190 0.66 +1.2 3,203,352 + tdcG NIAGMN_16190 0.66 +0.7 3,203,352 + tdcG NIAGMN_16190 0.66 -0.0 3,203,353 - tdcG NIAGMN_16190 0.66 +1.7 3,203,412 + tdcG NIAGMN_16190 0.70 +1.0 3,203,511 - tdcG NIAGMN_16190 0.78 +3.2 3,203,519 + tdcG NIAGMN_16190 0.78 +0.3 3,203,519 + tdcG NIAGMN_16190 0.78 +0.0 3,203,520 - tdcG NIAGMN_16190 0.78 -1.5 3,203,520 - tdcG NIAGMN_16190 0.78 -2.1 3,203,520 - tdcG NIAGMN_16190 0.78 -0.4 3,203,543 + tdcG NIAGMN_16190 0.80 +0.7 3,203,544 - tdcG NIAGMN_16190 0.80 -1.1 3,203,544 - tdcG NIAGMN_16190 0.80 +1.0 3,203,574 + tdcG NIAGMN_16190 0.82 -3.9 3,203,574 + tdcG NIAGMN_16190 0.82 -0.8 3,203,574 + tdcG NIAGMN_16190 0.82 +0.2 3,203,612 + tdcG NIAGMN_16190 0.85 +0.2 3,203,634 + tdcG NIAGMN_16190 0.87 -0.0 3,203,634 + tdcG NIAGMN_16190 0.87 +0.8 3,203,634 + tdcG NIAGMN_16190 0.87 +0.7 3,203,634 + tdcG NIAGMN_16190 0.87 -1.3 3,203,715 + -1.0 3,203,735 - -0.9 3,203,735 - -0.1 3,203,735 - +0.2 3,203,751 + -0.5 3,203,751 + +0.5 3,203,751 + +0.4 3,203,752 - -0.6 3,203,760 + -0.4 3,203,760 + +1.4 3,203,761 - -3.2 3,203,764 + +1.9 3,203,881 - -2.7 3,203,997 + -0.8 3,203,997 + +0.4 3,204,215 - -2.5 3,204,215 - -2.3 3,204,326 + dlsT NIAGMN_16195 0.18 -3.6 3,204,326 + dlsT NIAGMN_16195 0.18 -1.6 3,204,326 + dlsT NIAGMN_16195 0.18 -0.2 3,204,327 - dlsT NIAGMN_16195 0.18 +0.4 3,204,327 - dlsT NIAGMN_16195 0.18 -1.2 3,204,327 - dlsT NIAGMN_16195 0.18 -0.0 3,204,346 + dlsT NIAGMN_16195 0.19 -0.5 3,204,346 + dlsT NIAGMN_16195 0.19 +1.6 3,204,347 - dlsT NIAGMN_16195 0.19 -2.1 3,204,349 + dlsT NIAGMN_16195 0.20 +0.2 3,204,350 - dlsT NIAGMN_16195 0.20 -0.9 3,204,350 - dlsT NIAGMN_16195 0.20 +0.7 3,204,352 + dlsT NIAGMN_16195 0.20 -1.2 3,204,352 + dlsT NIAGMN_16195 0.20 +0.2 3,204,352 + dlsT NIAGMN_16195 0.20 +0.8 3,204,432 + dlsT NIAGMN_16195 0.26 +0.4 3,204,443 + dlsT NIAGMN_16195 0.27 -0.4 3,204,443 + dlsT NIAGMN_16195 0.27 +0.4 3,204,443 + dlsT NIAGMN_16195 0.27 -4.2 3,204,444 - dlsT NIAGMN_16195 0.27 -1.4 3,204,509 + dlsT NIAGMN_16195 0.32 -0.2 3,204,510 - dlsT NIAGMN_16195 0.32 +0.1 3,204,517 + dlsT NIAGMN_16195 0.32 +0.7 3,204,517 + dlsT NIAGMN_16195 0.32 -0.4 3,204,517 + dlsT NIAGMN_16195 0.32 -0.2 3,204,517 + dlsT NIAGMN_16195 0.32 +0.1 3,204,517 + dlsT NIAGMN_16195 0.32 -1.7 3,204,518 - dlsT NIAGMN_16195 0.32 +0.5 3,204,518 - dlsT NIAGMN_16195 0.32 -0.7 3,204,518 - dlsT NIAGMN_16195 0.32 -0.2 3,204,560 + dlsT NIAGMN_16195 0.35 +1.1 3,204,561 - dlsT NIAGMN_16195 0.35 -0.4 3,204,561 - dlsT NIAGMN_16195 0.35 -1.7 3,204,704 + dlsT NIAGMN_16195 0.46 -0.4 3,204,704 + dlsT NIAGMN_16195 0.46 -0.5 3,204,705 - dlsT NIAGMN_16195 0.46 +0.9 3,204,705 - dlsT NIAGMN_16195 0.46 +0.9 3,204,705 - dlsT NIAGMN_16195 0.46 -1.9 3,204,705 - dlsT NIAGMN_16195 0.46 +0.2 3,204,754 + dlsT NIAGMN_16195 0.50 +0.9 3,204,757 + dlsT NIAGMN_16195 0.50 -2.4 3,204,757 + dlsT NIAGMN_16195 0.50 -1.0 3,204,758 - dlsT NIAGMN_16195 0.50 +0.8 3,204,801 + dlsT NIAGMN_16195 0.53 +0.7 3,204,801 + dlsT NIAGMN_16195 0.53 +0.6
Or see this region's nucleotide sequence