Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_12910 and ilvN are separated by 3 nucleotides ilvN and NIAGMN_12920 are separated by 262 nucleotides NIAGMN_12920 and NIAGMN_12925 are separated by 147 nucleotides NIAGMN_12925 and NIAGMN_12930 are separated by 277 nucleotides
NIAGMN_12910: NIAGMN_12910 - Acetolactate synthase, at 2,558,473 to 2,560,161
_12910
NIAGMN_12915: ilvN - acetolactate synthase small subunit, at 2,560,165 to 2,560,455
ilvN
NIAGMN_12920: NIAGMN_12920 - hypothetical protein, at 2,560,718 to 2,561,371
_12920
NIAGMN_12925: NIAGMN_12925 - hypothetical protein, at 2,561,519 to 2,562,001
_12925
NIAGMN_12930: NIAGMN_12930 - hypothetical protein, at 2,562,279 to 2,562,593
_12930
Position (kb)
2560
2561
2562 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2559.822 kb on + strand, within NIAGMN_12910 at 2559.823 kb on - strand, within NIAGMN_12910 at 2559.823 kb on - strand, within NIAGMN_12910 at 2559.823 kb on - strand, within NIAGMN_12910 at 2559.865 kb on - strand, within NIAGMN_12910 at 2559.865 kb on - strand, within NIAGMN_12910 at 2559.926 kb on - strand, within NIAGMN_12910 at 2559.949 kb on - strand, within NIAGMN_12910 at 2559.953 kb on - strand, within NIAGMN_12910 at 2560.159 kb on + strand at 2560.317 kb on + strand, within ilvN at 2560.317 kb on + strand, within ilvN at 2560.317 kb on + strand, within ilvN at 2560.317 kb on + strand, within ilvN at 2560.318 kb on - strand, within ilvN at 2560.318 kb on - strand, within ilvN at 2560.318 kb on - strand, within ilvN at 2560.361 kb on + strand, within ilvN at 2560.361 kb on + strand, within ilvN at 2560.362 kb on - strand, within ilvN at 2560.362 kb on - strand, within ilvN at 2560.405 kb on - strand, within ilvN at 2560.405 kb on - strand, within ilvN at 2560.466 kb on + strand at 2560.484 kb on + strand at 2560.485 kb on - strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.518 kb on + strand at 2560.519 kb on - strand at 2560.519 kb on - strand at 2560.520 kb on + strand at 2560.520 kb on + strand at 2560.520 kb on + strand at 2560.520 kb on + strand at 2560.520 kb on + strand at 2560.521 kb on - strand at 2560.521 kb on - strand at 2560.521 kb on - strand at 2560.521 kb on - strand at 2560.522 kb on + strand at 2560.522 kb on + strand at 2560.522 kb on + strand at 2560.522 kb on + strand at 2560.523 kb on - strand at 2560.583 kb on - strand at 2560.616 kb on + strand at 2560.616 kb on + strand at 2560.643 kb on + strand at 2560.663 kb on + strand at 2560.715 kb on - strand at 2560.885 kb on - strand, within NIAGMN_12920 at 2560.919 kb on - strand, within NIAGMN_12920 at 2561.258 kb on - strand, within NIAGMN_12920 at 2562.105 kb on + strand at 2562.105 kb on + strand at 2562.105 kb on + strand at 2562.106 kb on - strand at 2562.106 kb on - strand at 2562.106 kb on - strand at 2562.115 kb on + strand at 2562.160 kb on + strand at 2562.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,559,822 + NIAGMN_12910 0.80 -0.1 2,559,823 - NIAGMN_12910 0.80 -1.8 2,559,823 - NIAGMN_12910 0.80 -2.8 2,559,823 - NIAGMN_12910 0.80 -1.5 2,559,865 - NIAGMN_12910 0.82 +0.9 2,559,865 - NIAGMN_12910 0.82 +0.6 2,559,926 - NIAGMN_12910 0.86 +0.8 2,559,949 - NIAGMN_12910 0.87 -1.2 2,559,953 - NIAGMN_12910 0.88 -0.7 2,560,159 + +0.4 2,560,317 + ilvN NIAGMN_12915 0.52 +1.2 2,560,317 + ilvN NIAGMN_12915 0.52 +0.6 2,560,317 + ilvN NIAGMN_12915 0.52 +0.7 2,560,317 + ilvN NIAGMN_12915 0.52 +1.8 2,560,318 - ilvN NIAGMN_12915 0.53 -0.3 2,560,318 - ilvN NIAGMN_12915 0.53 +1.0 2,560,318 - ilvN NIAGMN_12915 0.53 +0.9 2,560,361 + ilvN NIAGMN_12915 0.67 +0.4 2,560,361 + ilvN NIAGMN_12915 0.67 +1.1 2,560,362 - ilvN NIAGMN_12915 0.68 -0.1 2,560,362 - ilvN NIAGMN_12915 0.68 +1.5 2,560,405 - ilvN NIAGMN_12915 0.82 -3.3 2,560,405 - ilvN NIAGMN_12915 0.82 -0.9 2,560,466 + +0.7 2,560,484 + +1.1 2,560,485 - -2.8 2,560,518 + +1.3 2,560,518 + +1.3 2,560,518 + -1.4 2,560,518 + -1.0 2,560,518 + +0.4 2,560,518 + +1.8 2,560,518 + +0.4 2,560,518 + -0.1 2,560,518 + +0.4 2,560,519 - +0.7 2,560,519 - +1.1 2,560,520 + +0.9 2,560,520 + -0.8 2,560,520 + -0.1 2,560,520 + -3.0 2,560,520 + -0.2 2,560,521 - +1.9 2,560,521 - +0.4 2,560,521 - -2.5 2,560,521 - +0.1 2,560,522 + -0.4 2,560,522 + +0.1 2,560,522 + -1.5 2,560,522 + +0.4 2,560,523 - -1.6 2,560,583 - +0.0 2,560,616 + -1.0 2,560,616 + -0.4 2,560,643 + -0.4 2,560,663 + -0.2 2,560,715 - +0.0 2,560,885 - NIAGMN_12920 0.26 +0.0 2,560,919 - NIAGMN_12920 0.31 +0.1 2,561,258 - NIAGMN_12920 0.83 -1.8 2,562,105 + +0.4 2,562,105 + -0.2 2,562,105 + -1.3 2,562,106 - -0.6 2,562,106 - +1.4 2,562,106 - +1.8 2,562,115 + -1.8 2,562,160 + -0.4 2,562,237 - -2.9
Or see this region's nucleotide sequence