Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnaA and tnaC are separated by 220 nucleotides tnaC and NIAGMN_12730 are separated by 399 nucleotides NIAGMN_12730 and NIAGMN_12735 overlap by 50 nucleotides NIAGMN_12735 and NIAGMN_12740 are separated by 149 nucleotides
NIAGMN_12720: tnaA - tryptophanase, at 2,522,878 to 2,524,293
tnaA
NIAGMN_12725: tnaC - tryptophanase leader peptide, at 2,524,514 to 2,524,588
tnaC
NIAGMN_12730: NIAGMN_12730 - toxin, at 2,524,988 to 2,526,976
_12730
NIAGMN_12735: NIAGMN_12735 - hypothetical protein, at 2,526,927 to 2,527,157
_12735
NIAGMN_12740: NIAGMN_12740 - tRNA modification GTPase MnmE, at 2,527,307 to 2,528,671
_12740
Position (kb)
2524
2525
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2524.010 kb on + strand, within tnaA at 2524.010 kb on + strand, within tnaA at 2524.010 kb on + strand, within tnaA at 2524.011 kb on - strand, within tnaA at 2524.052 kb on + strand, within tnaA at 2524.052 kb on + strand, within tnaA at 2524.052 kb on + strand, within tnaA at 2524.052 kb on + strand, within tnaA at 2524.053 kb on - strand, within tnaA at 2524.055 kb on + strand, within tnaA at 2524.055 kb on + strand, within tnaA at 2524.055 kb on + strand, within tnaA at 2524.056 kb on - strand, within tnaA at 2524.056 kb on - strand, within tnaA at 2524.107 kb on + strand, within tnaA at 2524.108 kb on - strand, within tnaA at 2524.108 kb on - strand, within tnaA at 2524.144 kb on + strand, within tnaA at 2524.294 kb on + strand at 2524.371 kb on - strand at 2524.448 kb on + strand at 2524.501 kb on - strand at 2524.572 kb on - strand, within tnaC at 2524.573 kb on + strand, within tnaC at 2524.573 kb on + strand, within tnaC at 2524.573 kb on + strand, within tnaC at 2524.573 kb on + strand, within tnaC at 2524.574 kb on - strand, within tnaC at 2524.578 kb on - strand, within tnaC at 2524.578 kb on - strand, within tnaC at 2524.623 kb on + strand at 2524.624 kb on - strand at 2524.795 kb on + strand at 2524.807 kb on + strand at 2524.808 kb on - strand at 2524.939 kb on + strand at 2525.016 kb on + strand at 2525.016 kb on + strand at 2525.023 kb on - strand at 2525.092 kb on - strand at 2525.259 kb on + strand, within NIAGMN_12730 at 2525.281 kb on + strand, within NIAGMN_12730 at 2525.283 kb on + strand, within NIAGMN_12730 at 2525.283 kb on + strand, within NIAGMN_12730 at 2525.284 kb on - strand, within NIAGMN_12730 at 2525.286 kb on + strand, within NIAGMN_12730 at 2525.286 kb on + strand, within NIAGMN_12730 at 2525.291 kb on - strand, within NIAGMN_12730 at 2525.291 kb on - strand, within NIAGMN_12730 at 2525.351 kb on + strand, within NIAGMN_12730 at 2525.540 kb on + strand, within NIAGMN_12730 at 2525.541 kb on - strand, within NIAGMN_12730 at 2525.570 kb on - strand, within NIAGMN_12730 at 2525.664 kb on + strand, within NIAGMN_12730 at 2525.665 kb on - strand, within NIAGMN_12730 at 2525.707 kb on + strand, within NIAGMN_12730 at 2525.708 kb on - strand, within NIAGMN_12730 at 2525.708 kb on - strand, within NIAGMN_12730 at 2525.805 kb on - strand, within NIAGMN_12730 at 2525.884 kb on - strand, within NIAGMN_12730 at 2526.031 kb on - strand, within NIAGMN_12730 at 2526.040 kb on - strand, within NIAGMN_12730 at 2526.171 kb on + strand, within NIAGMN_12730 at 2526.273 kb on + strand, within NIAGMN_12730 at 2526.460 kb on + strand, within NIAGMN_12730 at 2526.618 kb on + strand, within NIAGMN_12730 at 2526.619 kb on - strand, within NIAGMN_12730 at 2526.643 kb on + strand, within NIAGMN_12730 at 2526.745 kb on + strand, within NIAGMN_12730 at 2526.765 kb on - strand, within NIAGMN_12730 at 2526.807 kb on + strand at 2526.808 kb on - strand at 2526.931 kb on + strand at 2526.931 kb on + strand at 2526.932 kb on - strand at 2526.932 kb on - strand at 2526.932 kb on - strand at 2526.933 kb on + strand at 2526.934 kb on - strand at 2527.043 kb on - strand, within NIAGMN_12735 at 2527.045 kb on + strand, within NIAGMN_12735 at 2527.155 kb on + strand at 2527.199 kb on + strand at 2527.199 kb on + strand at 2527.200 kb on - strand at 2527.228 kb on - strand at 2527.255 kb on + strand at 2527.259 kb on + strand at 2527.260 kb on - strand at 2527.289 kb on + strand at 2527.289 kb on + strand at 2527.290 kb on - strand at 2527.290 kb on - strand at 2527.290 kb on - strand at 2527.290 kb on - strand at 2527.300 kb on - strand at 2527.308 kb on + strand at 2527.308 kb on + strand at 2527.308 kb on + strand at 2527.309 kb on - strand at 2527.309 kb on - strand at 2527.309 kb on - strand at 2527.309 kb on - strand at 2527.309 kb on - strand at 2527.309 kb on - strand at 2527.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,524,010 + tnaA NIAGMN_12720 0.80 -1.6 2,524,010 + tnaA NIAGMN_12720 0.80 +0.8 2,524,010 + tnaA NIAGMN_12720 0.80 +0.1 2,524,011 - tnaA NIAGMN_12720 0.80 +1.9 2,524,052 + tnaA NIAGMN_12720 0.83 -0.4 2,524,052 + tnaA NIAGMN_12720 0.83 -0.1 2,524,052 + tnaA NIAGMN_12720 0.83 +1.4 2,524,052 + tnaA NIAGMN_12720 0.83 +0.6 2,524,053 - tnaA NIAGMN_12720 0.83 -0.4 2,524,055 + tnaA NIAGMN_12720 0.83 +0.6 2,524,055 + tnaA NIAGMN_12720 0.83 -0.1 2,524,055 + tnaA NIAGMN_12720 0.83 +0.6 2,524,056 - tnaA NIAGMN_12720 0.83 +0.0 2,524,056 - tnaA NIAGMN_12720 0.83 +1.1 2,524,107 + tnaA NIAGMN_12720 0.87 +1.3 2,524,108 - tnaA NIAGMN_12720 0.87 -1.8 2,524,108 - tnaA NIAGMN_12720 0.87 +0.3 2,524,144 + tnaA NIAGMN_12720 0.89 -0.8 2,524,294 + +1.4 2,524,371 - +0.6 2,524,448 + +0.8 2,524,501 - +0.9 2,524,572 - tnaC NIAGMN_12725 0.77 -0.0 2,524,573 + tnaC NIAGMN_12725 0.79 -0.6 2,524,573 + tnaC NIAGMN_12725 0.79 +1.3 2,524,573 + tnaC NIAGMN_12725 0.79 -3.1 2,524,573 + tnaC NIAGMN_12725 0.79 -0.6 2,524,574 - tnaC NIAGMN_12725 0.80 +0.2 2,524,578 - tnaC NIAGMN_12725 0.85 +0.3 2,524,578 - tnaC NIAGMN_12725 0.85 -0.4 2,524,623 + +0.9 2,524,624 - +0.3 2,524,795 + +0.1 2,524,807 + +1.7 2,524,808 - -2.7 2,524,939 + +1.3 2,525,016 + +1.9 2,525,016 + +0.2 2,525,023 - -0.5 2,525,092 - +0.5 2,525,259 + NIAGMN_12730 0.14 -0.5 2,525,281 + NIAGMN_12730 0.15 +0.3 2,525,283 + NIAGMN_12730 0.15 +0.7 2,525,283 + NIAGMN_12730 0.15 +0.9 2,525,284 - NIAGMN_12730 0.15 -0.4 2,525,286 + NIAGMN_12730 0.15 -0.2 2,525,286 + NIAGMN_12730 0.15 +0.0 2,525,291 - NIAGMN_12730 0.15 -0.2 2,525,291 - NIAGMN_12730 0.15 +0.0 2,525,351 + NIAGMN_12730 0.18 -1.0 2,525,540 + NIAGMN_12730 0.28 +0.9 2,525,541 - NIAGMN_12730 0.28 +2.1 2,525,570 - NIAGMN_12730 0.29 +1.3 2,525,664 + NIAGMN_12730 0.34 -2.0 2,525,665 - NIAGMN_12730 0.34 +1.1 2,525,707 + NIAGMN_12730 0.36 +0.8 2,525,708 - NIAGMN_12730 0.36 -3.2 2,525,708 - NIAGMN_12730 0.36 +0.8 2,525,805 - NIAGMN_12730 0.41 -1.3 2,525,884 - NIAGMN_12730 0.45 +0.2 2,526,031 - NIAGMN_12730 0.52 +1.6 2,526,040 - NIAGMN_12730 0.53 -1.0 2,526,171 + NIAGMN_12730 0.59 -2.0 2,526,273 + NIAGMN_12730 0.65 -0.8 2,526,460 + NIAGMN_12730 0.74 +1.1 2,526,618 + NIAGMN_12730 0.82 +0.1 2,526,619 - NIAGMN_12730 0.82 +1.0 2,526,643 + NIAGMN_12730 0.83 +0.7 2,526,745 + NIAGMN_12730 0.88 -0.4 2,526,765 - NIAGMN_12730 0.89 +0.8 2,526,807 + +1.2 2,526,808 - -0.4 2,526,931 + +2.4 2,526,931 + +0.7 2,526,932 - +0.6 2,526,932 - +1.0 2,526,932 - -0.6 2,526,933 + +0.1 2,526,934 - +0.8 2,527,043 - NIAGMN_12735 0.50 +0.2 2,527,045 + NIAGMN_12735 0.51 +0.0 2,527,155 + +1.9 2,527,199 + +0.5 2,527,199 + -2.1 2,527,200 - +1.0 2,527,228 - +1.1 2,527,255 + -0.8 2,527,259 + +1.4 2,527,260 - -1.0 2,527,289 + +1.0 2,527,289 + -2.1 2,527,290 - -0.0 2,527,290 - -0.5 2,527,290 - -0.3 2,527,290 - +2.2 2,527,300 - +0.6 2,527,308 + +0.2 2,527,308 + -1.3 2,527,308 + +0.0 2,527,309 - +0.9 2,527,309 - -2.2 2,527,309 - +0.3 2,527,309 - +0.7 2,527,309 - +0.6 2,527,309 - +0.3 2,527,313 - -0.2
Or see this region's nucleotide sequence