Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paeA and ytfK are separated by 95 nucleotides ytfK and ytfJ are separated by 324 nucleotides ytfJ and ytfI are separated by 62 nucleotides
NIAGMN_09865: paeA - Polyamine export protein, at 1,943,789 to 1,945,132
paeA
NIAGMN_09870: ytfK - Uncharacterized protein YtfK, at 1,945,228 to 1,945,434
ytfK
NIAGMN_09875: ytfJ - Uncharacterized protein YtfJ, at 1,945,759 to 1,946,313
ytfJ
NIAGMN_09880: ytfI - Uncharacterized protein YtfI, at 1,946,376 to 1,947,314
ytfI
Position (kb)
1945
1946
1947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1944.923 kb on + strand, within paeA at 1944.944 kb on - strand, within paeA at 1944.944 kb on - strand, within paeA at 1944.952 kb on + strand, within paeA at 1944.952 kb on + strand, within paeA at 1944.953 kb on - strand, within paeA at 1944.953 kb on - strand, within paeA at 1944.961 kb on + strand, within paeA at 1944.961 kb on + strand, within paeA at 1944.962 kb on - strand, within paeA at 1945.001 kb on + strand at 1945.027 kb on + strand at 1945.027 kb on + strand at 1945.031 kb on + strand at 1945.031 kb on + strand at 1945.031 kb on + strand at 1945.031 kb on + strand at 1945.031 kb on + strand at 1945.105 kb on + strand at 1945.112 kb on - strand at 1945.131 kb on - strand at 1945.140 kb on - strand at 1945.173 kb on + strand at 1945.174 kb on - strand at 1945.174 kb on - strand at 1945.183 kb on + strand at 1945.184 kb on - strand at 1945.200 kb on + strand at 1945.218 kb on - strand at 1945.218 kb on - strand at 1945.218 kb on - strand at 1945.218 kb on - strand at 1945.218 kb on - strand at 1945.218 kb on - strand at 1945.266 kb on + strand, within ytfK at 1945.297 kb on - strand, within ytfK at 1945.297 kb on - strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.361 kb on + strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.362 kb on - strand, within ytfK at 1945.363 kb on + strand, within ytfK at 1945.364 kb on - strand, within ytfK at 1945.364 kb on - strand, within ytfK at 1945.387 kb on + strand, within ytfK at 1945.391 kb on + strand, within ytfK at 1945.392 kb on - strand, within ytfK at 1945.392 kb on - strand, within ytfK at 1945.415 kb on + strand at 1945.438 kb on + strand at 1945.438 kb on + strand at 1945.439 kb on - strand at 1945.452 kb on + strand at 1945.453 kb on - strand at 1945.455 kb on + strand at 1945.456 kb on - strand at 1945.486 kb on + strand at 1945.486 kb on + strand at 1945.487 kb on - strand at 1945.487 kb on - strand at 1945.487 kb on - strand at 1945.487 kb on - strand at 1945.512 kb on + strand at 1945.512 kb on + strand at 1945.513 kb on - strand at 1945.513 kb on - strand at 1945.513 kb on - strand at 1945.513 kb on - strand at 1945.521 kb on + strand at 1945.521 kb on + strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.522 kb on - strand at 1945.563 kb on - strand at 1945.566 kb on + strand at 1945.590 kb on - strand at 1945.617 kb on + strand at 1945.619 kb on + strand at 1945.619 kb on + strand at 1945.619 kb on + strand at 1945.619 kb on + strand at 1945.620 kb on - strand at 1945.620 kb on - strand at 1945.620 kb on - strand at 1945.620 kb on - strand at 1945.692 kb on + strand at 1945.692 kb on + strand at 1945.693 kb on - strand at 1945.699 kb on + strand at 1945.699 kb on + strand at 1945.699 kb on + strand at 1945.699 kb on + strand at 1945.699 kb on + strand at 1945.700 kb on - strand at 1945.700 kb on - strand at 1945.700 kb on - strand at 1945.700 kb on - strand at 1945.700 kb on - strand at 1945.700 kb on - strand at 1945.705 kb on + strand at 1945.705 kb on + strand at 1945.739 kb on + strand at 1945.739 kb on + strand at 1945.740 kb on - strand at 1945.744 kb on + strand at 1945.744 kb on + strand at 1945.744 kb on + strand at 1945.745 kb on - strand at 1945.766 kb on + strand at 1945.767 kb on - strand at 1945.887 kb on + strand, within ytfJ at 1945.903 kb on + strand, within ytfJ at 1945.903 kb on + strand, within ytfJ at 1945.904 kb on - strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.956 kb on + strand, within ytfJ at 1945.957 kb on - strand, within ytfJ at 1945.957 kb on - strand, within ytfJ at 1946.010 kb on + strand, within ytfJ at 1946.011 kb on - strand, within ytfJ at 1946.059 kb on - strand, within ytfJ at 1946.116 kb on - strand, within ytfJ at 1946.118 kb on + strand, within ytfJ at 1946.119 kb on - strand, within ytfJ at 1946.146 kb on - strand, within ytfJ at 1946.208 kb on - strand, within ytfJ at 1946.321 kb on - strand at 1946.362 kb on - strand at 1946.377 kb on + strand at 1946.403 kb on + strand at 1946.454 kb on - strand at 1946.454 kb on - strand at 1946.512 kb on - strand, within ytfI at 1946.674 kb on - strand, within ytfI at 1946.680 kb on - strand, within ytfI at 1946.701 kb on - strand, within ytfI at 1946.715 kb on - strand, within ytfI at 1946.717 kb on - strand, within ytfI at 1946.758 kb on - strand, within ytfI at 1946.788 kb on - strand, within ytfI at 1946.791 kb on - strand, within ytfI at 1946.791 kb on - strand, within ytfI at 1946.792 kb on + strand, within ytfI at 1946.902 kb on + strand, within ytfI at 1946.903 kb on - strand, within ytfI at 1946.903 kb on - strand, within ytfI at 1947.112 kb on + strand, within ytfI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,944,923 + paeA NIAGMN_09865 0.84 +1.2 1,944,944 - paeA NIAGMN_09865 0.86 -1.0 1,944,944 - paeA NIAGMN_09865 0.86 -0.3 1,944,952 + paeA NIAGMN_09865 0.87 +2.0 1,944,952 + paeA NIAGMN_09865 0.87 -0.8 1,944,953 - paeA NIAGMN_09865 0.87 +0.4 1,944,953 - paeA NIAGMN_09865 0.87 -0.7 1,944,961 + paeA NIAGMN_09865 0.87 +1.0 1,944,961 + paeA NIAGMN_09865 0.87 +1.4 1,944,962 - paeA NIAGMN_09865 0.87 +1.2 1,945,001 + -1.1 1,945,027 + -1.4 1,945,027 + -3.4 1,945,031 + -0.3 1,945,031 + +0.4 1,945,031 + +0.5 1,945,031 + -0.5 1,945,031 + +0.2 1,945,105 + +0.4 1,945,112 - -0.7 1,945,131 - -2.0 1,945,140 - +0.2 1,945,173 + -0.1 1,945,174 - -0.6 1,945,174 - +1.0 1,945,183 + +2.0 1,945,184 - -0.3 1,945,200 + +1.0 1,945,218 - -0.3 1,945,218 - -0.0 1,945,218 - +1.5 1,945,218 - -0.2 1,945,218 - +0.2 1,945,218 - +0.4 1,945,266 + ytfK NIAGMN_09870 0.18 +1.1 1,945,297 - ytfK NIAGMN_09870 0.33 +1.3 1,945,297 - ytfK NIAGMN_09870 0.33 +1.0 1,945,361 + ytfK NIAGMN_09870 0.64 +0.4 1,945,361 + ytfK NIAGMN_09870 0.64 +1.2 1,945,361 + ytfK NIAGMN_09870 0.64 +1.0 1,945,361 + ytfK NIAGMN_09870 0.64 +1.1 1,945,361 + ytfK NIAGMN_09870 0.64 -3.3 1,945,361 + ytfK NIAGMN_09870 0.64 -0.5 1,945,361 + ytfK NIAGMN_09870 0.64 -2.3 1,945,361 + ytfK NIAGMN_09870 0.64 +1.4 1,945,361 + ytfK NIAGMN_09870 0.64 +0.7 1,945,361 + ytfK NIAGMN_09870 0.64 +2.0 1,945,361 + ytfK NIAGMN_09870 0.64 +2.5 1,945,361 + ytfK NIAGMN_09870 0.64 -0.4 1,945,362 - ytfK NIAGMN_09870 0.65 -1.6 1,945,362 - ytfK NIAGMN_09870 0.65 +0.2 1,945,362 - ytfK NIAGMN_09870 0.65 +0.4 1,945,362 - ytfK NIAGMN_09870 0.65 +0.9 1,945,362 - ytfK NIAGMN_09870 0.65 +1.1 1,945,362 - ytfK NIAGMN_09870 0.65 +0.7 1,945,362 - ytfK NIAGMN_09870 0.65 +1.9 1,945,362 - ytfK NIAGMN_09870 0.65 +1.4 1,945,362 - ytfK NIAGMN_09870 0.65 -1.1 1,945,362 - ytfK NIAGMN_09870 0.65 +1.7 1,945,362 - ytfK NIAGMN_09870 0.65 +0.6 1,945,363 + ytfK NIAGMN_09870 0.65 +1.3 1,945,364 - ytfK NIAGMN_09870 0.66 +1.2 1,945,364 - ytfK NIAGMN_09870 0.66 +1.0 1,945,387 + ytfK NIAGMN_09870 0.77 -0.2 1,945,391 + ytfK NIAGMN_09870 0.79 +1.2 1,945,392 - ytfK NIAGMN_09870 0.79 +0.9 1,945,392 - ytfK NIAGMN_09870 0.79 -1.0 1,945,415 + +1.3 1,945,438 + +1.0 1,945,438 + +1.0 1,945,439 - +0.6 1,945,452 + -3.5 1,945,453 - +0.9 1,945,455 + +1.3 1,945,456 - -0.2 1,945,486 + +1.2 1,945,486 + -1.0 1,945,487 - -1.5 1,945,487 - +0.6 1,945,487 - -1.3 1,945,487 - -2.7 1,945,512 + +1.4 1,945,512 + +0.1 1,945,513 - +0.4 1,945,513 - +0.1 1,945,513 - +0.5 1,945,513 - +0.5 1,945,521 + +0.3 1,945,521 + +1.3 1,945,522 - -0.7 1,945,522 - +0.0 1,945,522 - +1.8 1,945,522 - -1.0 1,945,522 - -0.1 1,945,522 - +1.5 1,945,522 - +1.7 1,945,522 - +1.2 1,945,563 - +0.8 1,945,566 + -0.8 1,945,590 - +0.4 1,945,617 + -2.8 1,945,619 + -0.5 1,945,619 + -0.3 1,945,619 + -0.9 1,945,619 + +1.1 1,945,620 - +0.0 1,945,620 - +0.2 1,945,620 - -0.8 1,945,620 - +0.2 1,945,692 + +0.2 1,945,692 + -3.0 1,945,693 - +0.5 1,945,699 + -2.5 1,945,699 + +1.5 1,945,699 + -0.4 1,945,699 + +0.2 1,945,699 + +0.4 1,945,700 - +0.1 1,945,700 - -0.1 1,945,700 - -0.6 1,945,700 - -0.6 1,945,700 - +0.4 1,945,700 - -0.3 1,945,705 + -1.1 1,945,705 + +0.3 1,945,739 + -2.1 1,945,739 + +0.4 1,945,740 - +1.4 1,945,744 + +2.0 1,945,744 + +0.0 1,945,744 + +0.3 1,945,745 - -2.1 1,945,766 + +0.3 1,945,767 - -0.0 1,945,887 + ytfJ NIAGMN_09875 0.23 -3.3 1,945,903 + ytfJ NIAGMN_09875 0.26 +1.0 1,945,903 + ytfJ NIAGMN_09875 0.26 -0.0 1,945,904 - ytfJ NIAGMN_09875 0.26 +0.1 1,945,956 + ytfJ NIAGMN_09875 0.35 -1.2 1,945,956 + ytfJ NIAGMN_09875 0.35 +0.4 1,945,956 + ytfJ NIAGMN_09875 0.35 -1.9 1,945,956 + ytfJ NIAGMN_09875 0.35 +0.7 1,945,956 + ytfJ NIAGMN_09875 0.35 -0.6 1,945,956 + ytfJ NIAGMN_09875 0.35 +1.9 1,945,956 + ytfJ NIAGMN_09875 0.35 -1.1 1,945,956 + ytfJ NIAGMN_09875 0.35 +1.1 1,945,957 - ytfJ NIAGMN_09875 0.36 +0.0 1,945,957 - ytfJ NIAGMN_09875 0.36 -1.3 1,946,010 + ytfJ NIAGMN_09875 0.45 +0.4 1,946,011 - ytfJ NIAGMN_09875 0.45 +1.0 1,946,059 - ytfJ NIAGMN_09875 0.54 -1.3 1,946,116 - ytfJ NIAGMN_09875 0.64 -0.3 1,946,118 + ytfJ NIAGMN_09875 0.65 +0.5 1,946,119 - ytfJ NIAGMN_09875 0.65 +1.0 1,946,146 - ytfJ NIAGMN_09875 0.70 +1.6 1,946,208 - ytfJ NIAGMN_09875 0.81 -1.3 1,946,321 - -0.5 1,946,362 - -2.2 1,946,377 + +0.2 1,946,403 + -1.6 1,946,454 - -0.3 1,946,454 - +0.5 1,946,512 - ytfI NIAGMN_09880 0.14 -0.2 1,946,674 - ytfI NIAGMN_09880 0.32 -1.2 1,946,680 - ytfI NIAGMN_09880 0.32 -1.1 1,946,701 - ytfI NIAGMN_09880 0.35 -0.7 1,946,715 - ytfI NIAGMN_09880 0.36 -0.3 1,946,717 - ytfI NIAGMN_09880 0.36 -1.2 1,946,758 - ytfI NIAGMN_09880 0.41 -2.6 1,946,788 - ytfI NIAGMN_09880 0.44 +0.8 1,946,791 - ytfI NIAGMN_09880 0.44 -0.4 1,946,791 - ytfI NIAGMN_09880 0.44 -3.4 1,946,792 + ytfI NIAGMN_09880 0.44 -0.5 1,946,902 + ytfI NIAGMN_09880 0.56 +1.6 1,946,903 - ytfI NIAGMN_09880 0.56 +0.2 1,946,903 - ytfI NIAGMN_09880 0.56 +1.0 1,947,112 + ytfI NIAGMN_09880 0.78 -0.1
Or see this region's nucleotide sequence