Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bdcA and ridA are separated by 130 nucleotides ridA and pyrB are separated by 418 nucleotides pyrB and pyrI are separated by 12 nucleotides pyrI and ridA are separated by 72 nucleotides
NIAGMN_09705: bdcA - SDR family oxidoreductase, at 1,910,887 to 1,911,600
bdcA
NIAGMN_09710: ridA - Endoribonuclease L-PSP, at 1,911,731 to 1,912,126
ridA
NIAGMN_09715: pyrB - aspartate carbamoyltransferase, at 1,912,545 to 1,913,480
pyrB
NIAGMN_09720: pyrI - aspartate carbamoyltransferase regulatory subunit, at 1,913,493 to 1,913,954
pyrI
NIAGMN_09725: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 1,914,027 to 1,914,413
ridA
Position (kb) 1912
1913
1914 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1911.573 kb on + strand at 1911.573 kb on + strand at 1911.573 kb on + strand at 1911.573 kb on + strand at 1911.574 kb on - strand at 1911.606 kb on + strand at 1911.616 kb on + strand at 1911.654 kb on - strand at 1911.654 kb on - strand at 1911.689 kb on + strand at 1911.689 kb on + strand at 1911.690 kb on - strand at 1911.691 kb on + strand at 1911.691 kb on + strand at 1911.692 kb on - strand at 1911.735 kb on + strand at 1911.735 kb on + strand at 1911.735 kb on + strand at 1911.736 kb on - strand at 1911.776 kb on + strand, within ridA at 1911.777 kb on - strand, within ridA at 1911.791 kb on + strand, within ridA at 1911.791 kb on + strand, within ridA at 1911.791 kb on + strand, within ridA at 1911.792 kb on - strand, within ridA at 1911.840 kb on + strand, within ridA at 1911.840 kb on + strand, within ridA at 1911.841 kb on - strand, within ridA at 1911.841 kb on - strand, within ridA at 1911.841 kb on - strand, within ridA at 1911.950 kb on - strand, within ridA at 1911.962 kb on - strand, within ridA at 1911.973 kb on + strand, within ridA at 1912.066 kb on + strand, within ridA at 1912.067 kb on - strand, within ridA at 1912.067 kb on - strand, within ridA at 1912.067 kb on - strand, within ridA at 1912.067 kb on - strand, within ridA at 1912.096 kb on + strand at 1912.124 kb on + strand at 1912.124 kb on + strand at 1912.125 kb on - strand at 1912.125 kb on - strand at 1912.131 kb on + strand at 1912.132 kb on - strand at 1912.163 kb on + strand at 1912.164 kb on - strand at 1912.164 kb on - strand at 1912.164 kb on - strand at 1912.165 kb on + strand at 1912.166 kb on - strand at 1912.166 kb on - strand at 1912.242 kb on + strand at 1912.242 kb on + strand at 1912.242 kb on + strand at 1912.296 kb on + strand at 1912.297 kb on - strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.406 kb on + strand at 1912.407 kb on - strand at 1912.407 kb on - strand at 1912.407 kb on - strand at 1912.407 kb on - strand at 1912.407 kb on - strand at 1912.540 kb on - strand at 1912.559 kb on - strand at 1912.561 kb on - strand at 1912.561 kb on - strand at 1912.572 kb on - strand at 1912.572 kb on - strand at 1912.592 kb on + strand at 1912.781 kb on + strand, within pyrB at 1912.781 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.839 kb on + strand, within pyrB at 1912.840 kb on - strand, within pyrB at 1912.853 kb on - strand, within pyrB at 1912.912 kb on + strand, within pyrB at 1912.912 kb on + strand, within pyrB at 1912.912 kb on + strand, within pyrB at 1912.912 kb on + strand, within pyrB at 1912.913 kb on - strand, within pyrB at 1912.913 kb on - strand, within pyrB at 1912.972 kb on + strand, within pyrB at 1912.972 kb on + strand, within pyrB at 1912.973 kb on - strand, within pyrB at 1912.973 kb on - strand, within pyrB at 1912.973 kb on - strand, within pyrB at 1913.040 kb on + strand, within pyrB at 1913.040 kb on + strand, within pyrB at 1913.100 kb on + strand, within pyrB at 1913.101 kb on - strand, within pyrB at 1913.101 kb on - strand, within pyrB at 1913.136 kb on + strand, within pyrB at 1913.137 kb on - strand, within pyrB at 1913.137 kb on - strand, within pyrB at 1913.137 kb on - strand, within pyrB at 1913.189 kb on + strand, within pyrB at 1913.223 kb on + strand, within pyrB at 1913.223 kb on + strand, within pyrB at 1913.223 kb on + strand, within pyrB at 1913.224 kb on - strand, within pyrB at 1913.224 kb on - strand, within pyrB at 1913.328 kb on - strand, within pyrB at 1913.335 kb on + strand, within pyrB at 1913.336 kb on - strand, within pyrB at 1913.381 kb on + strand, within pyrB at 1913.381 kb on + strand, within pyrB at 1913.382 kb on - strand, within pyrB at 1913.382 kb on - strand, within pyrB at 1913.400 kb on + strand at 1913.400 kb on + strand at 1913.473 kb on + strand at 1913.478 kb on + strand at 1913.479 kb on - strand at 1913.488 kb on + strand at 1913.488 kb on + strand at 1913.488 kb on + strand at 1913.507 kb on + strand at 1913.542 kb on + strand, within pyrI at 1913.543 kb on - strand, within pyrI at 1913.573 kb on + strand, within pyrI at 1913.573 kb on + strand, within pyrI at 1913.704 kb on + strand, within pyrI at 1913.705 kb on - strand, within pyrI at 1913.705 kb on - strand, within pyrI at 1913.705 kb on - strand, within pyrI at 1913.705 kb on - strand, within pyrI at 1913.721 kb on + strand, within pyrI at 1913.721 kb on + strand, within pyrI at 1913.722 kb on - strand, within pyrI at 1913.757 kb on + strand, within pyrI at 1913.757 kb on + strand, within pyrI at 1913.771 kb on + strand, within pyrI at 1913.772 kb on - strand, within pyrI at 1913.834 kb on + strand, within pyrI at 1913.834 kb on + strand, within pyrI at 1913.834 kb on + strand, within pyrI at 1913.834 kb on + strand, within pyrI at 1913.835 kb on - strand, within pyrI at 1913.910 kb on + strand at 1913.911 kb on - strand at 1913.933 kb on + strand at 1913.952 kb on + strand at 1913.965 kb on + strand at 1913.965 kb on + strand at 1913.968 kb on + strand at 1913.968 kb on + strand at 1913.984 kb on + strand at 1913.984 kb on + strand at 1913.985 kb on - strand at 1913.987 kb on - strand at 1913.987 kb on - strand at 1914.011 kb on + strand at 1914.011 kb on + strand at 1914.012 kb on - strand at 1914.014 kb on + strand at 1914.066 kb on - strand, within ridA at 1914.075 kb on + strand, within ridA at 1914.075 kb on + strand, within ridA at 1914.076 kb on - strand, within ridA at 1914.079 kb on + strand, within ridA at 1914.080 kb on - strand, within ridA at 1914.080 kb on - strand, within ridA at 1914.080 kb on - strand, within ridA at 1914.101 kb on + strand, within ridA at 1914.101 kb on + strand, within ridA at 1914.101 kb on + strand, within ridA at 1914.101 kb on + strand, within ridA at 1914.101 kb on + strand, within ridA at 1914.102 kb on - strand, within ridA at 1914.102 kb on - strand, within ridA at 1914.102 kb on - strand, within ridA at 1914.260 kb on - strand, within ridA at 1914.297 kb on + strand, within ridA at 1914.297 kb on + strand, within ridA at 1914.298 kb on - strand, within ridA at 1914.433 kb on + strand at 1914.447 kb on + strand at 1914.447 kb on + strand at 1914.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,911,573 + -0.3 1,911,573 + +1.1 1,911,573 + +0.4 1,911,573 + -1.1 1,911,574 - +1.3 1,911,606 + +1.4 1,911,616 + +1.7 1,911,654 - -3.2 1,911,654 - +0.7 1,911,689 + -2.6 1,911,689 + +0.5 1,911,690 - -1.8 1,911,691 + +0.8 1,911,691 + +0.5 1,911,692 - +0.1 1,911,735 + -0.2 1,911,735 + +0.1 1,911,735 + +0.4 1,911,736 - -0.8 1,911,776 + ridA NIAGMN_09710 0.11 +1.5 1,911,777 - ridA NIAGMN_09710 0.12 +1.2 1,911,791 + ridA NIAGMN_09710 0.15 +1.4 1,911,791 + ridA NIAGMN_09710 0.15 -0.2 1,911,791 + ridA NIAGMN_09710 0.15 +1.3 1,911,792 - ridA NIAGMN_09710 0.15 -3.0 1,911,840 + ridA NIAGMN_09710 0.28 -1.2 1,911,840 + ridA NIAGMN_09710 0.28 +0.6 1,911,841 - ridA NIAGMN_09710 0.28 +0.9 1,911,841 - ridA NIAGMN_09710 0.28 -3.3 1,911,841 - ridA NIAGMN_09710 0.28 +0.6 1,911,950 - ridA NIAGMN_09710 0.55 -0.4 1,911,962 - ridA NIAGMN_09710 0.58 -0.3 1,911,973 + ridA NIAGMN_09710 0.61 +0.5 1,912,066 + ridA NIAGMN_09710 0.85 -0.1 1,912,067 - ridA NIAGMN_09710 0.85 +0.8 1,912,067 - ridA NIAGMN_09710 0.85 +1.7 1,912,067 - ridA NIAGMN_09710 0.85 -0.2 1,912,067 - ridA NIAGMN_09710 0.85 +0.0 1,912,096 + +1.8 1,912,124 + +0.2 1,912,124 + +0.1 1,912,125 - +1.5 1,912,125 - -0.0 1,912,131 + +0.1 1,912,132 - +1.3 1,912,163 + +0.8 1,912,164 - -1.1 1,912,164 - -0.1 1,912,164 - +0.4 1,912,165 + +1.0 1,912,166 - -1.7 1,912,166 - -0.5 1,912,242 + -1.0 1,912,242 + -1.9 1,912,242 + +0.6 1,912,296 + +0.4 1,912,297 - -1.4 1,912,406 + -0.1 1,912,406 + -0.1 1,912,406 + -3.4 1,912,406 + +0.6 1,912,406 + +0.3 1,912,406 + -0.4 1,912,406 + -1.5 1,912,406 + +0.4 1,912,407 - -0.9 1,912,407 - -1.4 1,912,407 - -1.1 1,912,407 - -1.8 1,912,407 - -2.2 1,912,540 - -3.2 1,912,559 - -2.6 1,912,561 - -3.7 1,912,561 - -2.3 1,912,572 - -4.3 1,912,572 - -1.1 1,912,592 + -3.0 1,912,781 + pyrB NIAGMN_09715 0.25 -1.3 1,912,781 + pyrB NIAGMN_09715 0.25 -3.1 1,912,839 + pyrB NIAGMN_09715 0.31 -2.4 1,912,839 + pyrB NIAGMN_09715 0.31 -3.1 1,912,839 + pyrB NIAGMN_09715 0.31 -4.8 1,912,839 + pyrB NIAGMN_09715 0.31 -0.2 1,912,839 + pyrB NIAGMN_09715 0.31 -1.2 1,912,839 + pyrB NIAGMN_09715 0.31 -3.0 1,912,840 - pyrB NIAGMN_09715 0.32 -3.0 1,912,853 - pyrB NIAGMN_09715 0.33 -2.3 1,912,912 + pyrB NIAGMN_09715 0.39 -1.6 1,912,912 + pyrB NIAGMN_09715 0.39 -2.7 1,912,912 + pyrB NIAGMN_09715 0.39 -2.9 1,912,912 + pyrB NIAGMN_09715 0.39 -4.4 1,912,913 - pyrB NIAGMN_09715 0.39 -4.5 1,912,913 - pyrB NIAGMN_09715 0.39 -3.5 1,912,972 + pyrB NIAGMN_09715 0.46 -2.2 1,912,972 + pyrB NIAGMN_09715 0.46 -2.1 1,912,973 - pyrB NIAGMN_09715 0.46 -1.9 1,912,973 - pyrB NIAGMN_09715 0.46 -0.7 1,912,973 - pyrB NIAGMN_09715 0.46 -2.8 1,913,040 + pyrB NIAGMN_09715 0.53 -3.1 1,913,040 + pyrB NIAGMN_09715 0.53 -5.1 1,913,100 + pyrB NIAGMN_09715 0.59 -3.4 1,913,101 - pyrB NIAGMN_09715 0.59 -3.7 1,913,101 - pyrB NIAGMN_09715 0.59 -2.1 1,913,136 + pyrB NIAGMN_09715 0.63 -3.3 1,913,137 - pyrB NIAGMN_09715 0.63 -2.4 1,913,137 - pyrB NIAGMN_09715 0.63 -3.4 1,913,137 - pyrB NIAGMN_09715 0.63 -3.8 1,913,189 + pyrB NIAGMN_09715 0.69 -3.1 1,913,223 + pyrB NIAGMN_09715 0.72 -2.6 1,913,223 + pyrB NIAGMN_09715 0.72 -5.9 1,913,223 + pyrB NIAGMN_09715 0.72 -2.9 1,913,224 - pyrB NIAGMN_09715 0.73 -2.2 1,913,224 - pyrB NIAGMN_09715 0.73 -1.4 1,913,328 - pyrB NIAGMN_09715 0.84 -2.2 1,913,335 + pyrB NIAGMN_09715 0.84 -4.4 1,913,336 - pyrB NIAGMN_09715 0.85 -4.4 1,913,381 + pyrB NIAGMN_09715 0.89 -3.9 1,913,381 + pyrB NIAGMN_09715 0.89 -2.5 1,913,382 - pyrB NIAGMN_09715 0.89 -1.2 1,913,382 - pyrB NIAGMN_09715 0.89 -2.5 1,913,400 + -2.0 1,913,400 + -2.7 1,913,473 + +2.0 1,913,478 + -3.1 1,913,479 - -1.8 1,913,488 + -3.3 1,913,488 + +0.7 1,913,488 + -1.8 1,913,507 + +0.3 1,913,542 + pyrI NIAGMN_09720 0.11 -0.8 1,913,543 - pyrI NIAGMN_09720 0.11 -2.9 1,913,573 + pyrI NIAGMN_09720 0.17 +1.5 1,913,573 + pyrI NIAGMN_09720 0.17 -1.5 1,913,704 + pyrI NIAGMN_09720 0.46 -1.1 1,913,705 - pyrI NIAGMN_09720 0.46 -1.0 1,913,705 - pyrI NIAGMN_09720 0.46 +0.0 1,913,705 - pyrI NIAGMN_09720 0.46 -0.4 1,913,705 - pyrI NIAGMN_09720 0.46 +1.6 1,913,721 + pyrI NIAGMN_09720 0.49 -2.1 1,913,721 + pyrI NIAGMN_09720 0.49 -3.2 1,913,722 - pyrI NIAGMN_09720 0.50 -1.1 1,913,757 + pyrI NIAGMN_09720 0.57 -1.0 1,913,757 + pyrI NIAGMN_09720 0.57 +0.2 1,913,771 + pyrI NIAGMN_09720 0.60 -1.2 1,913,772 - pyrI NIAGMN_09720 0.60 -0.2 1,913,834 + pyrI NIAGMN_09720 0.74 -0.8 1,913,834 + pyrI NIAGMN_09720 0.74 -0.9 1,913,834 + pyrI NIAGMN_09720 0.74 -1.0 1,913,834 + pyrI NIAGMN_09720 0.74 +1.3 1,913,835 - pyrI NIAGMN_09720 0.74 -0.7 1,913,910 + +4.0 1,913,911 - +0.2 1,913,933 + +0.2 1,913,952 + -0.6 1,913,965 + +0.6 1,913,965 + -0.9 1,913,968 + -0.2 1,913,968 + -0.2 1,913,984 + -1.3 1,913,984 + +0.4 1,913,985 - -2.8 1,913,987 - -0.8 1,913,987 - -0.1 1,914,011 + +1.9 1,914,011 + -2.1 1,914,012 - -1.0 1,914,014 + +0.3 1,914,066 - ridA NIAGMN_09725 0.10 +2.0 1,914,075 + ridA NIAGMN_09725 0.12 +0.2 1,914,075 + ridA NIAGMN_09725 0.12 +0.7 1,914,076 - ridA NIAGMN_09725 0.13 -1.0 1,914,079 + ridA NIAGMN_09725 0.13 -0.5 1,914,080 - ridA NIAGMN_09725 0.14 -1.0 1,914,080 - ridA NIAGMN_09725 0.14 +0.3 1,914,080 - ridA NIAGMN_09725 0.14 -0.6 1,914,101 + ridA NIAGMN_09725 0.19 -1.1 1,914,101 + ridA NIAGMN_09725 0.19 +0.0 1,914,101 + ridA NIAGMN_09725 0.19 +0.5 1,914,101 + ridA NIAGMN_09725 0.19 +0.3 1,914,101 + ridA NIAGMN_09725 0.19 +0.8 1,914,102 - ridA NIAGMN_09725 0.19 -3.6 1,914,102 - ridA NIAGMN_09725 0.19 -1.3 1,914,102 - ridA NIAGMN_09725 0.19 +0.9 1,914,260 - ridA NIAGMN_09725 0.60 -0.7 1,914,297 + ridA NIAGMN_09725 0.70 +1.3 1,914,297 + ridA NIAGMN_09725 0.70 +0.4 1,914,298 - ridA NIAGMN_09725 0.70 -0.7 1,914,433 + -1.6 1,914,447 + +0.6 1,914,447 + +0.5 1,914,448 - -1.2
Or see this region's nucleotide sequence