Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zorA and zorB are separated by 6 nucleotides zorB and zorE are separated by 3 nucleotides zorE and nanM are separated by 245 nucleotides nanM and NIAGMN_09555 overlap by 14 nucleotides
NIAGMN_09535: zorA - Zorya protein ZorA, at 1,876,824 to 1,878,476
zorA
NIAGMN_09540: zorB - Zorya protein ZorB, at 1,878,483 to 1,879,190
zorB
NIAGMN_09545: zorE - Zorya protein ZorE, at 1,879,194 to 1,880,297
zorE
NIAGMN_09550: nanM - N-acetylneuraminic acid mutarotase, at 1,880,543 to 1,880,851
nanM
NIAGMN_09555: NIAGMN_09555 - HNH endonuclease, at 1,880,838 to 1,881,689
_09555
Position (kb)
1879
1880
1881 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1878.224 kb on + strand, within zorA at 1878.299 kb on - strand, within zorA at 1878.353 kb on - strand at 1878.396 kb on + strand at 1878.396 kb on + strand at 1878.495 kb on - strand at 1878.590 kb on - strand, within zorB at 1878.590 kb on - strand, within zorB at 1878.600 kb on + strand, within zorB at 1878.614 kb on - strand, within zorB at 1878.712 kb on + strand, within zorB at 1878.779 kb on - strand, within zorB at 1878.878 kb on + strand, within zorB at 1878.911 kb on + strand, within zorB at 1878.911 kb on + strand, within zorB at 1878.957 kb on - strand, within zorB at 1878.959 kb on + strand, within zorB at 1878.980 kb on + strand, within zorB at 1879.115 kb on + strand, within zorB at 1879.205 kb on + strand at 1879.223 kb on + strand at 1879.299 kb on - strand at 1879.371 kb on + strand, within zorE at 1879.371 kb on + strand, within zorE at 1879.372 kb on - strand, within zorE at 1879.397 kb on + strand, within zorE at 1879.436 kb on - strand, within zorE at 1879.537 kb on + strand, within zorE at 1879.893 kb on - strand, within zorE at 1879.958 kb on + strand, within zorE at 1879.960 kb on + strand, within zorE at 1880.021 kb on - strand, within zorE at 1880.233 kb on + strand at 1880.234 kb on - strand at 1880.429 kb on + strand at 1880.430 kb on - strand at 1880.434 kb on + strand at 1880.434 kb on + strand at 1880.434 kb on + strand at 1880.466 kb on + strand at 1880.588 kb on + strand, within nanM at 1880.589 kb on - strand, within nanM at 1880.668 kb on + strand, within nanM at 1880.668 kb on + strand, within nanM at 1880.668 kb on + strand, within nanM at 1880.669 kb on - strand, within nanM at 1880.670 kb on + strand, within nanM at 1880.816 kb on + strand, within nanM at 1880.916 kb on - strand at 1880.926 kb on + strand, within NIAGMN_09555 at 1880.927 kb on - strand, within NIAGMN_09555 at 1880.933 kb on - strand, within NIAGMN_09555 at 1880.935 kb on - strand, within NIAGMN_09555 at 1880.939 kb on - strand, within NIAGMN_09555 at 1880.939 kb on - strand, within NIAGMN_09555 at 1880.939 kb on - strand, within NIAGMN_09555 at 1880.995 kb on - strand, within NIAGMN_09555 at 1881.073 kb on + strand, within NIAGMN_09555 at 1881.073 kb on + strand, within NIAGMN_09555 at 1881.074 kb on - strand, within NIAGMN_09555 at 1881.097 kb on + strand, within NIAGMN_09555 at 1881.097 kb on + strand, within NIAGMN_09555 at 1881.097 kb on + strand, within NIAGMN_09555 at 1881.097 kb on + strand, within NIAGMN_09555 at 1881.098 kb on - strand, within NIAGMN_09555 at 1881.098 kb on - strand, within NIAGMN_09555 at 1881.098 kb on - strand, within NIAGMN_09555 at 1881.198 kb on + strand, within NIAGMN_09555 at 1881.198 kb on + strand, within NIAGMN_09555 at 1881.218 kb on + strand, within NIAGMN_09555 at 1881.233 kb on + strand, within NIAGMN_09555 at 1881.233 kb on + strand, within NIAGMN_09555 at 1881.276 kb on - strand, within NIAGMN_09555
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,878,224 + zorA NIAGMN_09535 0.85 +1.5 1,878,299 - zorA NIAGMN_09535 0.89 -1.3 1,878,353 - -2.0 1,878,396 + -0.7 1,878,396 + +0.3 1,878,495 - +2.5 1,878,590 - zorB NIAGMN_09540 0.15 -0.5 1,878,590 - zorB NIAGMN_09540 0.15 -2.0 1,878,600 + zorB NIAGMN_09540 0.17 +0.9 1,878,614 - zorB NIAGMN_09540 0.19 +0.3 1,878,712 + zorB NIAGMN_09540 0.32 +2.6 1,878,779 - zorB NIAGMN_09540 0.42 +0.4 1,878,878 + zorB NIAGMN_09540 0.56 +1.6 1,878,911 + zorB NIAGMN_09540 0.60 +0.8 1,878,911 + zorB NIAGMN_09540 0.60 +0.1 1,878,957 - zorB NIAGMN_09540 0.67 -1.6 1,878,959 + zorB NIAGMN_09540 0.67 +1.0 1,878,980 + zorB NIAGMN_09540 0.70 +1.2 1,879,115 + zorB NIAGMN_09540 0.89 -0.8 1,879,205 + +0.8 1,879,223 + +0.7 1,879,299 - -1.5 1,879,371 + zorE NIAGMN_09545 0.16 -0.8 1,879,371 + zorE NIAGMN_09545 0.16 -0.3 1,879,372 - zorE NIAGMN_09545 0.16 +1.0 1,879,397 + zorE NIAGMN_09545 0.18 -0.7 1,879,436 - zorE NIAGMN_09545 0.22 +1.8 1,879,537 + zorE NIAGMN_09545 0.31 -2.0 1,879,893 - zorE NIAGMN_09545 0.63 +0.3 1,879,958 + zorE NIAGMN_09545 0.69 -0.4 1,879,960 + zorE NIAGMN_09545 0.69 -0.3 1,880,021 - zorE NIAGMN_09545 0.75 +0.7 1,880,233 + +0.6 1,880,234 - -0.5 1,880,429 + -0.1 1,880,430 - -1.7 1,880,434 + -4.0 1,880,434 + +0.2 1,880,434 + -0.0 1,880,466 + +0.4 1,880,588 + nanM NIAGMN_09550 0.15 +0.9 1,880,589 - nanM NIAGMN_09550 0.15 +0.3 1,880,668 + nanM NIAGMN_09550 0.40 +0.5 1,880,668 + nanM NIAGMN_09550 0.40 +1.0 1,880,668 + nanM NIAGMN_09550 0.40 +0.1 1,880,669 - nanM NIAGMN_09550 0.41 -1.0 1,880,670 + nanM NIAGMN_09550 0.41 +0.2 1,880,816 + nanM NIAGMN_09550 0.88 +0.0 1,880,916 - +0.1 1,880,926 + NIAGMN_09555 0.10 -3.0 1,880,927 - NIAGMN_09555 0.10 +1.6 1,880,933 - NIAGMN_09555 0.11 -1.0 1,880,935 - NIAGMN_09555 0.11 +1.5 1,880,939 - NIAGMN_09555 0.12 +0.9 1,880,939 - NIAGMN_09555 0.12 -1.5 1,880,939 - NIAGMN_09555 0.12 -1.2 1,880,995 - NIAGMN_09555 0.18 -0.4 1,881,073 + NIAGMN_09555 0.28 +0.6 1,881,073 + NIAGMN_09555 0.28 +0.7 1,881,074 - NIAGMN_09555 0.28 +1.1 1,881,097 + NIAGMN_09555 0.30 -2.2 1,881,097 + NIAGMN_09555 0.30 +1.0 1,881,097 + NIAGMN_09555 0.30 +0.0 1,881,097 + NIAGMN_09555 0.30 -1.8 1,881,098 - NIAGMN_09555 0.31 +1.1 1,881,098 - NIAGMN_09555 0.31 +1.0 1,881,098 - NIAGMN_09555 0.31 +0.0 1,881,198 + NIAGMN_09555 0.42 +0.4 1,881,198 + NIAGMN_09555 0.42 -0.1 1,881,218 + NIAGMN_09555 0.45 -2.6 1,881,233 + NIAGMN_09555 0.46 -1.2 1,881,233 + NIAGMN_09555 0.46 +2.3 1,881,276 - NIAGMN_09555 0.51 +0.7
Or see this region's nucleotide sequence