Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rapA and rluA are separated by 11 nucleotides rluA and espY1 are separated by 417 nucleotides
NIAGMN_08650: rapA - RNA polymerase-associated protein RapA, at 1,673,848 to 1,676,754
rapA
NIAGMN_08655: rluA - bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA, at 1,676,766 to 1,677,425
rluA
NIAGMN_08660: espY1 - Type III secretion system effector EspY1, at 1,677,843 to 1,678,595
espY1
Position (kb)
1676
1677
1678 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1675.771 kb on - strand, within rapA at 1675.771 kb on - strand, within rapA at 1675.774 kb on + strand, within rapA at 1675.775 kb on - strand, within rapA at 1675.775 kb on - strand, within rapA at 1675.784 kb on + strand, within rapA at 1675.784 kb on + strand, within rapA at 1675.785 kb on - strand, within rapA at 1675.785 kb on - strand, within rapA at 1675.785 kb on - strand, within rapA at 1675.786 kb on + strand, within rapA at 1675.786 kb on + strand, within rapA at 1675.786 kb on + strand, within rapA at 1675.786 kb on + strand, within rapA at 1675.787 kb on - strand, within rapA at 1675.787 kb on - strand, within rapA at 1675.787 kb on - strand, within rapA at 1675.800 kb on + strand, within rapA at 1675.800 kb on + strand, within rapA at 1675.800 kb on + strand, within rapA at 1675.800 kb on + strand, within rapA at 1675.800 kb on + strand, within rapA at 1675.804 kb on + strand, within rapA at 1675.805 kb on - strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.082 kb on + strand, within rapA at 1676.083 kb on - strand, within rapA at 1676.176 kb on - strand, within rapA at 1676.176 kb on - strand, within rapA at 1676.283 kb on + strand, within rapA at 1676.283 kb on + strand, within rapA at 1676.283 kb on + strand, within rapA at 1676.284 kb on - strand, within rapA at 1676.305 kb on + strand, within rapA at 1676.306 kb on - strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.373 kb on + strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.374 kb on - strand, within rapA at 1676.385 kb on + strand, within rapA at 1676.414 kb on + strand, within rapA at 1676.414 kb on + strand, within rapA at 1676.415 kb on - strand, within rapA at 1676.415 kb on - strand, within rapA at 1676.415 kb on - strand, within rapA at 1676.684 kb on + strand at 1676.685 kb on - strand at 1676.741 kb on + strand at 1676.741 kb on + strand at 1676.753 kb on - strand at 1676.753 kb on - strand at 1676.782 kb on - strand at 1676.782 kb on - strand at 1676.831 kb on + strand at 1676.831 kb on + strand at 1676.831 kb on + strand at 1676.832 kb on - strand, within rluA at 1676.832 kb on - strand, within rluA at 1676.832 kb on - strand, within rluA at 1676.832 kb on - strand, within rluA at 1676.834 kb on + strand, within rluA at 1676.834 kb on + strand, within rluA at 1676.834 kb on + strand, within rluA at 1676.835 kb on - strand, within rluA at 1676.835 kb on - strand, within rluA at 1676.835 kb on - strand, within rluA at 1676.835 kb on - strand, within rluA at 1676.980 kb on + strand, within rluA at 1676.980 kb on + strand, within rluA at 1677.051 kb on + strand, within rluA at 1677.051 kb on + strand, within rluA at 1677.051 kb on + strand, within rluA at 1677.051 kb on + strand, within rluA at 1677.051 kb on + strand, within rluA at 1677.180 kb on + strand, within rluA at 1677.180 kb on + strand, within rluA at 1677.195 kb on + strand, within rluA at 1677.195 kb on + strand, within rluA at 1677.226 kb on + strand, within rluA at 1677.266 kb on + strand, within rluA at 1677.267 kb on - strand, within rluA at 1677.267 kb on - strand, within rluA at 1677.267 kb on - strand, within rluA at 1677.306 kb on + strand, within rluA at 1677.307 kb on - strand, within rluA at 1677.349 kb on + strand, within rluA at 1677.349 kb on + strand, within rluA at 1677.349 kb on + strand, within rluA at 1677.349 kb on + strand, within rluA at 1677.392 kb on + strand at 1677.395 kb on + strand at 1677.395 kb on + strand at 1677.395 kb on + strand at 1677.395 kb on + strand at 1677.396 kb on - strand at 1677.405 kb on - strand at 1677.441 kb on + strand at 1677.441 kb on + strand at 1677.442 kb on - strand at 1677.442 kb on - strand at 1677.444 kb on - strand at 1677.454 kb on + strand at 1677.471 kb on - strand at 1677.471 kb on - strand at 1677.474 kb on + strand at 1677.474 kb on + strand at 1677.474 kb on + strand at 1677.475 kb on - strand at 1677.537 kb on - strand at 1677.613 kb on + strand at 1677.614 kb on - strand at 1677.614 kb on - strand at 1677.614 kb on - strand at 1677.614 kb on - strand at 1677.615 kb on + strand at 1677.615 kb on + strand at 1677.638 kb on - strand at 1677.638 kb on - strand at 1677.657 kb on - strand at 1677.657 kb on - strand at 1677.672 kb on - strand at 1677.714 kb on - strand at 1677.745 kb on + strand at 1677.745 kb on + strand at 1677.746 kb on - strand at 1677.746 kb on - strand at 1677.746 kb on - strand at 1677.768 kb on - strand at 1677.795 kb on + strand at 1677.796 kb on - strand at 1677.830 kb on - strand at 1677.830 kb on - strand at 1677.882 kb on - strand at 1677.882 kb on - strand at 1677.941 kb on + strand, within espY1 at 1677.942 kb on - strand, within espY1 at 1677.945 kb on + strand, within espY1 at 1677.955 kb on + strand, within espY1 at 1677.955 kb on + strand, within espY1 at 1677.956 kb on - strand, within espY1 at 1677.957 kb on + strand, within espY1 at 1677.957 kb on + strand, within espY1 at 1677.957 kb on + strand, within espY1 at 1677.957 kb on + strand, within espY1 at 1677.958 kb on - strand, within espY1 at 1677.958 kb on - strand, within espY1 at 1677.966 kb on - strand, within espY1 at 1678.011 kb on - strand, within espY1 at 1678.011 kb on - strand, within espY1 at 1678.026 kb on - strand, within espY1 at 1678.091 kb on - strand, within espY1 at 1678.091 kb on - strand, within espY1 at 1678.120 kb on + strand, within espY1 at 1678.121 kb on - strand, within espY1 at 1678.130 kb on + strand, within espY1 at 1678.130 kb on + strand, within espY1 at 1678.186 kb on - strand, within espY1 at 1678.186 kb on - strand, within espY1 at 1678.186 kb on - strand, within espY1 at 1678.219 kb on - strand, within espY1 at 1678.257 kb on - strand, within espY1
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,675,771 - rapA NIAGMN_08650 0.66 -2.5 1,675,771 - rapA NIAGMN_08650 0.66 -0.8 1,675,774 + rapA NIAGMN_08650 0.66 -0.1 1,675,775 - rapA NIAGMN_08650 0.66 +1.2 1,675,775 - rapA NIAGMN_08650 0.66 -2.0 1,675,784 + rapA NIAGMN_08650 0.67 +0.1 1,675,784 + rapA NIAGMN_08650 0.67 +0.3 1,675,785 - rapA NIAGMN_08650 0.67 +0.1 1,675,785 - rapA NIAGMN_08650 0.67 +0.2 1,675,785 - rapA NIAGMN_08650 0.67 -2.3 1,675,786 + rapA NIAGMN_08650 0.67 -0.7 1,675,786 + rapA NIAGMN_08650 0.67 +1.0 1,675,786 + rapA NIAGMN_08650 0.67 -1.3 1,675,786 + rapA NIAGMN_08650 0.67 +0.8 1,675,787 - rapA NIAGMN_08650 0.67 -1.3 1,675,787 - rapA NIAGMN_08650 0.67 +0.5 1,675,787 - rapA NIAGMN_08650 0.67 -0.1 1,675,800 + rapA NIAGMN_08650 0.67 +0.6 1,675,800 + rapA NIAGMN_08650 0.67 -1.2 1,675,800 + rapA NIAGMN_08650 0.67 +1.8 1,675,800 + rapA NIAGMN_08650 0.67 -0.3 1,675,800 + rapA NIAGMN_08650 0.67 -1.3 1,675,804 + rapA NIAGMN_08650 0.67 -1.7 1,675,805 - rapA NIAGMN_08650 0.67 +0.8 1,676,082 + rapA NIAGMN_08650 0.77 +0.5 1,676,082 + rapA NIAGMN_08650 0.77 +0.6 1,676,082 + rapA NIAGMN_08650 0.77 +1.4 1,676,082 + rapA NIAGMN_08650 0.77 +0.2 1,676,082 + rapA NIAGMN_08650 0.77 +0.5 1,676,082 + rapA NIAGMN_08650 0.77 -1.2 1,676,082 + rapA NIAGMN_08650 0.77 -1.2 1,676,082 + rapA NIAGMN_08650 0.77 +0.0 1,676,083 - rapA NIAGMN_08650 0.77 +0.2 1,676,176 - rapA NIAGMN_08650 0.80 -0.8 1,676,176 - rapA NIAGMN_08650 0.80 -2.0 1,676,283 + rapA NIAGMN_08650 0.84 +0.2 1,676,283 + rapA NIAGMN_08650 0.84 -1.2 1,676,283 + rapA NIAGMN_08650 0.84 +1.2 1,676,284 - rapA NIAGMN_08650 0.84 -1.7 1,676,305 + rapA NIAGMN_08650 0.85 +1.1 1,676,306 - rapA NIAGMN_08650 0.85 +1.5 1,676,373 + rapA NIAGMN_08650 0.87 +1.3 1,676,373 + rapA NIAGMN_08650 0.87 -2.6 1,676,373 + rapA NIAGMN_08650 0.87 -0.6 1,676,373 + rapA NIAGMN_08650 0.87 -1.4 1,676,373 + rapA NIAGMN_08650 0.87 +0.4 1,676,373 + rapA NIAGMN_08650 0.87 +0.2 1,676,373 + rapA NIAGMN_08650 0.87 +0.1 1,676,373 + rapA NIAGMN_08650 0.87 -1.4 1,676,373 + rapA NIAGMN_08650 0.87 -0.8 1,676,374 - rapA NIAGMN_08650 0.87 -0.6 1,676,374 - rapA NIAGMN_08650 0.87 +1.1 1,676,374 - rapA NIAGMN_08650 0.87 +0.9 1,676,374 - rapA NIAGMN_08650 0.87 +0.1 1,676,374 - rapA NIAGMN_08650 0.87 +1.5 1,676,374 - rapA NIAGMN_08650 0.87 +1.7 1,676,374 - rapA NIAGMN_08650 0.87 +0.5 1,676,374 - rapA NIAGMN_08650 0.87 -0.8 1,676,374 - rapA NIAGMN_08650 0.87 +1.4 1,676,374 - rapA NIAGMN_08650 0.87 -1.7 1,676,385 + rapA NIAGMN_08650 0.87 -1.7 1,676,414 + rapA NIAGMN_08650 0.88 +0.5 1,676,414 + rapA NIAGMN_08650 0.88 +0.9 1,676,415 - rapA NIAGMN_08650 0.88 +1.6 1,676,415 - rapA NIAGMN_08650 0.88 -0.3 1,676,415 - rapA NIAGMN_08650 0.88 -0.5 1,676,684 + +1.0 1,676,685 - -0.2 1,676,741 + +0.6 1,676,741 + +1.7 1,676,753 - +2.0 1,676,753 - +0.1 1,676,782 - -2.2 1,676,782 - +1.7 1,676,831 + +0.1 1,676,831 + -0.3 1,676,831 + +0.5 1,676,832 - rluA NIAGMN_08655 0.10 -3.5 1,676,832 - rluA NIAGMN_08655 0.10 +0.6 1,676,832 - rluA NIAGMN_08655 0.10 +3.1 1,676,832 - rluA NIAGMN_08655 0.10 +1.9 1,676,834 + rluA NIAGMN_08655 0.10 -0.2 1,676,834 + rluA NIAGMN_08655 0.10 +0.4 1,676,834 + rluA NIAGMN_08655 0.10 +1.3 1,676,835 - rluA NIAGMN_08655 0.10 +0.1 1,676,835 - rluA NIAGMN_08655 0.10 +0.9 1,676,835 - rluA NIAGMN_08655 0.10 +0.6 1,676,835 - rluA NIAGMN_08655 0.10 -0.3 1,676,980 + rluA NIAGMN_08655 0.32 +0.0 1,676,980 + rluA NIAGMN_08655 0.32 +0.0 1,677,051 + rluA NIAGMN_08655 0.43 -0.8 1,677,051 + rluA NIAGMN_08655 0.43 -0.1 1,677,051 + rluA NIAGMN_08655 0.43 -0.9 1,677,051 + rluA NIAGMN_08655 0.43 -0.5 1,677,051 + rluA NIAGMN_08655 0.43 -0.3 1,677,180 + rluA NIAGMN_08655 0.63 -1.5 1,677,180 + rluA NIAGMN_08655 0.63 -2.7 1,677,195 + rluA NIAGMN_08655 0.65 -0.7 1,677,195 + rluA NIAGMN_08655 0.65 +1.4 1,677,226 + rluA NIAGMN_08655 0.70 -0.4 1,677,266 + rluA NIAGMN_08655 0.76 -1.7 1,677,267 - rluA NIAGMN_08655 0.76 -3.2 1,677,267 - rluA NIAGMN_08655 0.76 -0.3 1,677,267 - rluA NIAGMN_08655 0.76 +1.7 1,677,306 + rluA NIAGMN_08655 0.82 +0.1 1,677,307 - rluA NIAGMN_08655 0.82 +1.1 1,677,349 + rluA NIAGMN_08655 0.88 -0.6 1,677,349 + rluA NIAGMN_08655 0.88 -0.6 1,677,349 + rluA NIAGMN_08655 0.88 -1.6 1,677,349 + rluA NIAGMN_08655 0.88 -0.1 1,677,392 + +1.9 1,677,395 + +0.3 1,677,395 + -0.2 1,677,395 + -0.2 1,677,395 + +0.8 1,677,396 - +0.7 1,677,405 - -0.2 1,677,441 + +0.1 1,677,441 + +0.4 1,677,442 - -0.4 1,677,442 - +0.3 1,677,444 - -2.2 1,677,454 + +0.1 1,677,471 - -2.0 1,677,471 - +0.2 1,677,474 + -0.4 1,677,474 + +0.5 1,677,474 + -0.3 1,677,475 - +1.6 1,677,537 - -1.4 1,677,613 + -1.7 1,677,614 - -1.0 1,677,614 - +0.1 1,677,614 - -1.3 1,677,614 - +1.2 1,677,615 + -2.8 1,677,615 + -0.5 1,677,638 - +0.9 1,677,638 - +0.2 1,677,657 - -2.2 1,677,657 - -0.2 1,677,672 - +0.3 1,677,714 - +0.3 1,677,745 + +1.1 1,677,745 + +0.1 1,677,746 - -1.5 1,677,746 - +0.2 1,677,746 - +1.0 1,677,768 - -0.3 1,677,795 + -0.8 1,677,796 - -1.5 1,677,830 - +1.7 1,677,830 - -1.7 1,677,882 - -0.5 1,677,882 - -1.1 1,677,941 + espY1 NIAGMN_08660 0.13 -1.5 1,677,942 - espY1 NIAGMN_08660 0.13 +0.1 1,677,945 + espY1 NIAGMN_08660 0.14 -3.7 1,677,955 + espY1 NIAGMN_08660 0.15 +0.7 1,677,955 + espY1 NIAGMN_08660 0.15 -1.7 1,677,956 - espY1 NIAGMN_08660 0.15 -0.3 1,677,957 + espY1 NIAGMN_08660 0.15 -1.3 1,677,957 + espY1 NIAGMN_08660 0.15 -0.6 1,677,957 + espY1 NIAGMN_08660 0.15 -0.4 1,677,957 + espY1 NIAGMN_08660 0.15 -0.5 1,677,958 - espY1 NIAGMN_08660 0.15 +0.8 1,677,958 - espY1 NIAGMN_08660 0.15 -0.1 1,677,966 - espY1 NIAGMN_08660 0.16 +0.2 1,678,011 - espY1 NIAGMN_08660 0.22 -0.1 1,678,011 - espY1 NIAGMN_08660 0.22 +0.4 1,678,026 - espY1 NIAGMN_08660 0.24 +1.2 1,678,091 - espY1 NIAGMN_08660 0.33 +0.1 1,678,091 - espY1 NIAGMN_08660 0.33 +0.1 1,678,120 + espY1 NIAGMN_08660 0.37 -1.3 1,678,121 - espY1 NIAGMN_08660 0.37 +1.0 1,678,130 + espY1 NIAGMN_08660 0.38 +1.1 1,678,130 + espY1 NIAGMN_08660 0.38 -1.1 1,678,186 - espY1 NIAGMN_08660 0.46 +1.3 1,678,186 - espY1 NIAGMN_08660 0.46 +0.8 1,678,186 - espY1 NIAGMN_08660 0.46 +0.2 1,678,219 - espY1 NIAGMN_08660 0.50 +0.2 1,678,257 - espY1 NIAGMN_08660 0.55 -1.7
Or see this region's nucleotide sequence