Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadG and can are separated by 108 nucleotides can and hpt are separated by 40 nucleotides hpt and gcd are separated by 205 nucleotides
NIAGMN_08285: yadG - Uncharacterized ABC transporter ATP-binding protein YadG, at 1,593,967 to 1,594,893
yadG
NIAGMN_08290: can - carbonate dehydratase, at 1,595,002 to 1,595,664
can
NIAGMN_08295: hpt - hypoxanthine phosphoribosyltransferase, at 1,595,705 to 1,596,241
hpt
NIAGMN_08300: gcd - quinoprotein glucose dehydrogenase, at 1,596,447 to 1,598,837
gcd
Position (kb)
1595
1596
1597 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1594.725 kb on + strand, within yadG at 1594.744 kb on - strand, within yadG at 1594.744 kb on - strand, within yadG at 1594.905 kb on + strand at 1594.905 kb on + strand at 1594.906 kb on - strand at 1594.911 kb on - strand at 1594.918 kb on + strand at 1594.926 kb on - strand at 1595.662 kb on + strand at 1595.736 kb on + strand at 1595.737 kb on - strand at 1595.764 kb on - strand, within hpt at 1595.864 kb on - strand, within hpt at 1595.864 kb on - strand, within hpt at 1595.923 kb on + strand, within hpt at 1595.923 kb on + strand, within hpt at 1595.923 kb on + strand, within hpt at 1595.924 kb on - strand, within hpt at 1595.924 kb on - strand, within hpt at 1595.924 kb on - strand, within hpt at 1595.924 kb on - strand, within hpt at 1595.942 kb on - strand, within hpt at 1595.974 kb on + strand, within hpt at 1596.034 kb on - strand, within hpt at 1596.068 kb on + strand, within hpt at 1596.104 kb on - strand, within hpt at 1596.104 kb on - strand, within hpt at 1596.137 kb on - strand, within hpt at 1596.156 kb on + strand, within hpt at 1596.157 kb on - strand, within hpt at 1596.199 kb on + strand at 1596.222 kb on - strand at 1596.222 kb on - strand at 1596.222 kb on - strand at 1596.227 kb on + strand at 1596.228 kb on - strand at 1596.233 kb on - strand at 1596.321 kb on + strand at 1596.321 kb on + strand at 1596.322 kb on - strand at 1596.322 kb on - strand at 1596.323 kb on + strand at 1596.323 kb on + strand at 1596.324 kb on - strand at 1596.324 kb on - strand at 1596.324 kb on - strand at 1596.359 kb on - strand at 1596.404 kb on + strand at 1596.455 kb on + strand at 1596.461 kb on + strand at 1596.490 kb on + strand at 1596.522 kb on + strand at 1596.523 kb on - strand at 1596.523 kb on - strand at 1596.523 kb on - strand at 1596.523 kb on - strand at 1596.526 kb on + strand at 1596.527 kb on - strand at 1596.570 kb on + strand at 1596.571 kb on - strand at 1596.573 kb on + strand at 1596.574 kb on - strand at 1596.626 kb on + strand at 1596.627 kb on - strand at 1596.649 kb on - strand at 1596.842 kb on + strand, within gcd at 1596.842 kb on + strand, within gcd at 1596.843 kb on - strand, within gcd at 1596.845 kb on + strand, within gcd at 1596.846 kb on - strand, within gcd at 1596.846 kb on - strand, within gcd at 1596.867 kb on - strand, within gcd at 1596.867 kb on - strand, within gcd at 1596.895 kb on + strand, within gcd at 1596.923 kb on + strand, within gcd at 1596.923 kb on + strand, within gcd at 1596.924 kb on - strand, within gcd at 1596.957 kb on + strand, within gcd at 1596.957 kb on + strand, within gcd at 1596.958 kb on - strand, within gcd at 1596.958 kb on - strand, within gcd at 1597.049 kb on + strand, within gcd at 1597.234 kb on + strand, within gcd at 1597.235 kb on - strand, within gcd
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,594,725 + yadG NIAGMN_08285 0.82 +1.2 1,594,744 - yadG NIAGMN_08285 0.84 +0.2 1,594,744 - yadG NIAGMN_08285 0.84 +0.5 1,594,905 + -1.7 1,594,905 + +2.1 1,594,906 - -1.1 1,594,911 - +1.5 1,594,918 + -2.1 1,594,926 - -0.5 1,595,662 + -1.4 1,595,736 + +0.3 1,595,737 - -3.5 1,595,764 - hpt NIAGMN_08295 0.11 -1.7 1,595,864 - hpt NIAGMN_08295 0.30 +0.4 1,595,864 - hpt NIAGMN_08295 0.30 -0.2 1,595,923 + hpt NIAGMN_08295 0.41 +0.1 1,595,923 + hpt NIAGMN_08295 0.41 -0.2 1,595,923 + hpt NIAGMN_08295 0.41 -3.8 1,595,924 - hpt NIAGMN_08295 0.41 -1.2 1,595,924 - hpt NIAGMN_08295 0.41 +1.4 1,595,924 - hpt NIAGMN_08295 0.41 +1.2 1,595,924 - hpt NIAGMN_08295 0.41 +1.8 1,595,942 - hpt NIAGMN_08295 0.44 +0.4 1,595,974 + hpt NIAGMN_08295 0.50 +0.2 1,596,034 - hpt NIAGMN_08295 0.61 +0.3 1,596,068 + hpt NIAGMN_08295 0.68 -3.1 1,596,104 - hpt NIAGMN_08295 0.74 +0.4 1,596,104 - hpt NIAGMN_08295 0.74 -3.7 1,596,137 - hpt NIAGMN_08295 0.80 +0.1 1,596,156 + hpt NIAGMN_08295 0.84 -2.3 1,596,157 - hpt NIAGMN_08295 0.84 +0.5 1,596,199 + +1.4 1,596,222 - -0.6 1,596,222 - +0.3 1,596,222 - -5.2 1,596,227 + +0.5 1,596,228 - +0.6 1,596,233 - -1.0 1,596,321 + -1.2 1,596,321 + -0.2 1,596,322 - +1.2 1,596,322 - -0.6 1,596,323 + +0.3 1,596,323 + +1.7 1,596,324 - -0.6 1,596,324 - -0.2 1,596,324 - -1.0 1,596,359 - -1.6 1,596,404 + -0.0 1,596,455 + -1.0 1,596,461 + -1.3 1,596,490 + +0.1 1,596,522 + +0.0 1,596,523 - -0.3 1,596,523 - +0.4 1,596,523 - +0.7 1,596,523 - +0.1 1,596,526 + -0.4 1,596,527 - -0.0 1,596,570 + +0.0 1,596,571 - +0.8 1,596,573 + -1.1 1,596,574 - +0.1 1,596,626 + +0.2 1,596,627 - +0.6 1,596,649 - +0.4 1,596,842 + gcd NIAGMN_08300 0.17 +0.9 1,596,842 + gcd NIAGMN_08300 0.17 +0.1 1,596,843 - gcd NIAGMN_08300 0.17 +0.6 1,596,845 + gcd NIAGMN_08300 0.17 +0.3 1,596,846 - gcd NIAGMN_08300 0.17 +0.3 1,596,846 - gcd NIAGMN_08300 0.17 +0.2 1,596,867 - gcd NIAGMN_08300 0.18 -0.8 1,596,867 - gcd NIAGMN_08300 0.18 +0.4 1,596,895 + gcd NIAGMN_08300 0.19 -0.6 1,596,923 + gcd NIAGMN_08300 0.20 +0.4 1,596,923 + gcd NIAGMN_08300 0.20 +0.5 1,596,924 - gcd NIAGMN_08300 0.20 +0.6 1,596,957 + gcd NIAGMN_08300 0.21 +0.6 1,596,957 + gcd NIAGMN_08300 0.21 +0.5 1,596,958 - gcd NIAGMN_08300 0.21 +0.6 1,596,958 - gcd NIAGMN_08300 0.21 +0.4 1,597,049 + gcd NIAGMN_08300 0.25 +1.1 1,597,234 + gcd NIAGMN_08300 0.33 -1.6 1,597,235 - gcd NIAGMN_08300 0.33 -1.3
Or see this region's nucleotide sequence