Strain Fitness in Escherichia coli ECRC102 around NIAGMN_08215

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpcnB and folK overlap by 4 nucleotidesfolK and NIAGMN_08215 are separated by 280 nucleotidesNIAGMN_08215 and ecpD are separated by 86 nucleotidesecpD and fimD are separated by 41 nucleotides NIAGMN_08205: pcnB - polynucleotide adenylyltransferase PcnB, at 1,578,629 to 1,579,993 pcnB NIAGMN_08210: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 1,579,990 to 1,580,472 folK NIAGMN_08215: NIAGMN_08215 - fimbrial protein, at 1,580,753 to 1,581,349 _08215 NIAGMN_08220: ecpD - fimbrial chaperone, at 1,581,436 to 1,582,161 ecpD NIAGMN_08225: fimD - outer membrane usher protein, at 1,582,203 to 1,584,803 fimD Position (kb) 1580 1581 1582Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1579.780 kb on + strand, within pcnBat 1579.796 kb on + strand, within pcnBat 1579.945 kb on + strandat 1580.002 kb on + strandat 1580.002 kb on + strandat 1580.002 kb on + strandat 1580.002 kb on + strandat 1580.420 kb on + strand, within folKat 1580.468 kb on - strandat 1580.648 kb on + strandat 1580.702 kb on - strandat 1580.739 kb on + strandat 1580.811 kb on + strandat 1580.812 kb on - strandat 1580.840 kb on - strand, within NIAGMN_08215at 1580.845 kb on - strand, within NIAGMN_08215at 1580.845 kb on - strand, within NIAGMN_08215at 1580.845 kb on - strand, within NIAGMN_08215at 1580.860 kb on + strand, within NIAGMN_08215at 1580.874 kb on + strand, within NIAGMN_08215at 1580.874 kb on + strand, within NIAGMN_08215at 1580.874 kb on + strand, within NIAGMN_08215at 1580.875 kb on - strand, within NIAGMN_08215at 1580.878 kb on + strand, within NIAGMN_08215at 1580.884 kb on + strand, within NIAGMN_08215at 1580.885 kb on - strand, within NIAGMN_08215at 1580.923 kb on + strand, within NIAGMN_08215at 1580.942 kb on - strand, within NIAGMN_08215at 1581.040 kb on - strand, within NIAGMN_08215at 1581.059 kb on + strand, within NIAGMN_08215at 1581.091 kb on + strand, within NIAGMN_08215at 1581.092 kb on - strand, within NIAGMN_08215at 1581.113 kb on - strand, within NIAGMN_08215at 1581.148 kb on + strand, within NIAGMN_08215at 1581.181 kb on + strand, within NIAGMN_08215at 1581.242 kb on - strand, within NIAGMN_08215at 1581.251 kb on + strand, within NIAGMN_08215at 1581.255 kb on + strand, within NIAGMN_08215at 1581.256 kb on - strand, within NIAGMN_08215at 1581.260 kb on - strand, within NIAGMN_08215at 1581.260 kb on - strand, within NIAGMN_08215at 1581.269 kb on + strand, within NIAGMN_08215at 1581.313 kb on - strandat 1581.329 kb on + strandat 1581.330 kb on - strandat 1581.341 kb on + strandat 1581.341 kb on + strandat 1581.342 kb on - strandat 1581.342 kb on - strandat 1581.343 kb on + strandat 1581.343 kb on + strandat 1581.343 kb on + strandat 1581.343 kb on + strandat 1581.368 kb on + strandat 1581.517 kb on - strand, within ecpDat 1581.600 kb on - strand, within ecpDat 1581.727 kb on + strand, within ecpDat 1581.727 kb on + strand, within ecpDat 1581.727 kb on + strand, within ecpDat 1581.727 kb on + strand, within ecpDat 1581.728 kb on - strand, within ecpDat 1581.728 kb on - strand, within ecpDat 1581.728 kb on - strand, within ecpDat 1581.728 kb on - strand, within ecpDat 1581.954 kb on + strand, within ecpDat 1581.970 kb on + strand, within ecpDat 1581.970 kb on + strand, within ecpDat 1581.970 kb on + strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1581.971 kb on - strand, within ecpDat 1582.181 kb on + strandat 1582.207 kb on - strandat 1582.207 kb on - strandat 1582.249 kb on + strandat 1582.275 kb on + strandat 1582.278 kb on - strandat 1582.328 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,579,780 + pcnB NIAGMN_08205 0.84 +0.1
1,579,796 + pcnB NIAGMN_08205 0.85 -2.4
1,579,945 + -0.2
1,580,002 + -3.4
1,580,002 + -0.6
1,580,002 + +1.8
1,580,002 + -2.2
1,580,420 + folK NIAGMN_08210 0.89 +0.8
1,580,468 - +2.1
1,580,648 + -1.4
1,580,702 - +0.1
1,580,739 + -1.7
1,580,811 + +2.2
1,580,812 - -2.1
1,580,840 - NIAGMN_08215 0.15 +0.5
1,580,845 - NIAGMN_08215 0.15 -0.2
1,580,845 - NIAGMN_08215 0.15 -1.4
1,580,845 - NIAGMN_08215 0.15 +2.0
1,580,860 + NIAGMN_08215 0.18 +0.6
1,580,874 + NIAGMN_08215 0.20 -0.7
1,580,874 + NIAGMN_08215 0.20 -1.6
1,580,874 + NIAGMN_08215 0.20 +1.0
1,580,875 - NIAGMN_08215 0.20 +0.9
1,580,878 + NIAGMN_08215 0.21 -1.4
1,580,884 + NIAGMN_08215 0.22 +0.2
1,580,885 - NIAGMN_08215 0.22 -0.1
1,580,923 + NIAGMN_08215 0.28 -0.2
1,580,942 - NIAGMN_08215 0.32 -0.1
1,581,040 - NIAGMN_08215 0.48 +0.7
1,581,059 + NIAGMN_08215 0.51 +0.9
1,581,091 + NIAGMN_08215 0.57 +0.8
1,581,092 - NIAGMN_08215 0.57 +1.1
1,581,113 - NIAGMN_08215 0.60 -1.5
1,581,148 + NIAGMN_08215 0.66 -0.3
1,581,181 + NIAGMN_08215 0.72 +0.8
1,581,242 - NIAGMN_08215 0.82 -0.9
1,581,251 + NIAGMN_08215 0.83 +0.8
1,581,255 + NIAGMN_08215 0.84 +0.3
1,581,256 - NIAGMN_08215 0.84 -2.8
1,581,260 - NIAGMN_08215 0.85 -0.6
1,581,260 - NIAGMN_08215 0.85 +0.4
1,581,269 + NIAGMN_08215 0.86 -0.5
1,581,313 - +0.4
1,581,329 + +0.4
1,581,330 - +1.1
1,581,341 + -0.8
1,581,341 + +0.7
1,581,342 - -0.3
1,581,342 - +0.6
1,581,343 + -2.0
1,581,343 + +0.7
1,581,343 + -0.7
1,581,343 + -0.2
1,581,368 + -0.3
1,581,517 - ecpD NIAGMN_08220 0.11 +0.5
1,581,600 - ecpD NIAGMN_08220 0.23 -2.1
1,581,727 + ecpD NIAGMN_08220 0.40 +1.2
1,581,727 + ecpD NIAGMN_08220 0.40 -1.4
1,581,727 + ecpD NIAGMN_08220 0.40 +0.0
1,581,727 + ecpD NIAGMN_08220 0.40 +1.0
1,581,728 - ecpD NIAGMN_08220 0.40 +1.6
1,581,728 - ecpD NIAGMN_08220 0.40 +1.2
1,581,728 - ecpD NIAGMN_08220 0.40 +0.3
1,581,728 - ecpD NIAGMN_08220 0.40 -0.4
1,581,954 + ecpD NIAGMN_08220 0.71 +0.8
1,581,970 + ecpD NIAGMN_08220 0.74 +1.8
1,581,970 + ecpD NIAGMN_08220 0.74 +1.8
1,581,970 + ecpD NIAGMN_08220 0.74 +1.4
1,581,971 - ecpD NIAGMN_08220 0.74 -1.4
1,581,971 - ecpD NIAGMN_08220 0.74 -1.5
1,581,971 - ecpD NIAGMN_08220 0.74 +0.9
1,581,971 - ecpD NIAGMN_08220 0.74 -2.4
1,581,971 - ecpD NIAGMN_08220 0.74 -2.2
1,581,971 - ecpD NIAGMN_08220 0.74 -0.2
1,582,181 + -4.0
1,582,207 - -0.4
1,582,207 - +0.9
1,582,249 + -0.0
1,582,275 + -2.5
1,582,278 - +1.0
1,582,328 - -3.0

Or see this region's nucleotide sequence