Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and NIAGMN_08215 are separated by 280 nucleotides NIAGMN_08215 and ecpD are separated by 86 nucleotides
NIAGMN_08205: pcnB - polynucleotide adenylyltransferase PcnB, at 1,578,629 to 1,579,993
pcnB
NIAGMN_08210: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 1,579,990 to 1,580,472
folK
NIAGMN_08215: NIAGMN_08215 - fimbrial protein, at 1,580,753 to 1,581,349
_08215
NIAGMN_08220: ecpD - fimbrial chaperone, at 1,581,436 to 1,582,161
ecpD
Position (kb)
1579
1580
1581 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1579.100 kb on + strand, within pcnB at 1579.100 kb on + strand, within pcnB at 1579.133 kb on + strand, within pcnB at 1579.133 kb on + strand, within pcnB at 1579.133 kb on + strand, within pcnB at 1579.215 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.219 kb on + strand, within pcnB at 1579.382 kb on + strand, within pcnB at 1579.382 kb on + strand, within pcnB at 1579.384 kb on + strand, within pcnB at 1579.400 kb on + strand, within pcnB at 1579.732 kb on + strand, within pcnB at 1579.732 kb on + strand, within pcnB at 1579.732 kb on + strand, within pcnB at 1579.780 kb on + strand, within pcnB at 1579.796 kb on + strand, within pcnB at 1579.945 kb on + strand at 1580.002 kb on + strand at 1580.002 kb on + strand at 1580.002 kb on + strand at 1580.002 kb on + strand at 1580.420 kb on + strand, within folK at 1580.468 kb on - strand at 1580.648 kb on + strand at 1580.702 kb on - strand at 1580.739 kb on + strand at 1580.811 kb on + strand at 1580.812 kb on - strand at 1580.840 kb on - strand, within NIAGMN_08215 at 1580.845 kb on - strand, within NIAGMN_08215 at 1580.845 kb on - strand, within NIAGMN_08215 at 1580.845 kb on - strand, within NIAGMN_08215 at 1580.860 kb on + strand, within NIAGMN_08215 at 1580.874 kb on + strand, within NIAGMN_08215 at 1580.874 kb on + strand, within NIAGMN_08215 at 1580.874 kb on + strand, within NIAGMN_08215 at 1580.875 kb on - strand, within NIAGMN_08215 at 1580.878 kb on + strand, within NIAGMN_08215 at 1580.884 kb on + strand, within NIAGMN_08215 at 1580.885 kb on - strand, within NIAGMN_08215 at 1580.923 kb on + strand, within NIAGMN_08215 at 1580.942 kb on - strand, within NIAGMN_08215 at 1581.040 kb on - strand, within NIAGMN_08215 at 1581.059 kb on + strand, within NIAGMN_08215 at 1581.091 kb on + strand, within NIAGMN_08215 at 1581.092 kb on - strand, within NIAGMN_08215 at 1581.113 kb on - strand, within NIAGMN_08215 at 1581.148 kb on + strand, within NIAGMN_08215 at 1581.181 kb on + strand, within NIAGMN_08215 at 1581.242 kb on - strand, within NIAGMN_08215 at 1581.251 kb on + strand, within NIAGMN_08215 at 1581.255 kb on + strand, within NIAGMN_08215 at 1581.256 kb on - strand, within NIAGMN_08215 at 1581.260 kb on - strand, within NIAGMN_08215 at 1581.260 kb on - strand, within NIAGMN_08215 at 1581.269 kb on + strand, within NIAGMN_08215 at 1581.313 kb on - strand at 1581.329 kb on + strand at 1581.330 kb on - strand at 1581.341 kb on + strand at 1581.341 kb on + strand at 1581.342 kb on - strand at 1581.342 kb on - strand at 1581.343 kb on + strand at 1581.343 kb on + strand at 1581.343 kb on + strand at 1581.343 kb on + strand at 1581.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,579,100 + pcnB NIAGMN_08205 0.35 -0.4 1,579,100 + pcnB NIAGMN_08205 0.35 -2.9 1,579,133 + pcnB NIAGMN_08205 0.37 +0.3 1,579,133 + pcnB NIAGMN_08205 0.37 -1.2 1,579,133 + pcnB NIAGMN_08205 0.37 -3.0 1,579,215 + pcnB NIAGMN_08205 0.43 -2.1 1,579,219 + pcnB NIAGMN_08205 0.43 -0.1 1,579,219 + pcnB NIAGMN_08205 0.43 -2.5 1,579,219 + pcnB NIAGMN_08205 0.43 +2.5 1,579,219 + pcnB NIAGMN_08205 0.43 +0.5 1,579,219 + pcnB NIAGMN_08205 0.43 -1.5 1,579,219 + pcnB NIAGMN_08205 0.43 -2.7 1,579,219 + pcnB NIAGMN_08205 0.43 -0.9 1,579,382 + pcnB NIAGMN_08205 0.55 -1.6 1,579,382 + pcnB NIAGMN_08205 0.55 +1.7 1,579,384 + pcnB NIAGMN_08205 0.55 -0.4 1,579,400 + pcnB NIAGMN_08205 0.56 -2.3 1,579,732 + pcnB NIAGMN_08205 0.81 +1.1 1,579,732 + pcnB NIAGMN_08205 0.81 -1.5 1,579,732 + pcnB NIAGMN_08205 0.81 +1.6 1,579,780 + pcnB NIAGMN_08205 0.84 +0.1 1,579,796 + pcnB NIAGMN_08205 0.85 -2.4 1,579,945 + -0.2 1,580,002 + -3.4 1,580,002 + -0.6 1,580,002 + +1.8 1,580,002 + -2.2 1,580,420 + folK NIAGMN_08210 0.89 +0.8 1,580,468 - +2.1 1,580,648 + -1.4 1,580,702 - +0.1 1,580,739 + -1.7 1,580,811 + +2.2 1,580,812 - -2.1 1,580,840 - NIAGMN_08215 0.15 +0.5 1,580,845 - NIAGMN_08215 0.15 -0.2 1,580,845 - NIAGMN_08215 0.15 -1.4 1,580,845 - NIAGMN_08215 0.15 +2.0 1,580,860 + NIAGMN_08215 0.18 +0.6 1,580,874 + NIAGMN_08215 0.20 -0.7 1,580,874 + NIAGMN_08215 0.20 -1.6 1,580,874 + NIAGMN_08215 0.20 +1.0 1,580,875 - NIAGMN_08215 0.20 +0.9 1,580,878 + NIAGMN_08215 0.21 -1.4 1,580,884 + NIAGMN_08215 0.22 +0.2 1,580,885 - NIAGMN_08215 0.22 -0.1 1,580,923 + NIAGMN_08215 0.28 -0.2 1,580,942 - NIAGMN_08215 0.32 -0.1 1,581,040 - NIAGMN_08215 0.48 +0.7 1,581,059 + NIAGMN_08215 0.51 +0.9 1,581,091 + NIAGMN_08215 0.57 +0.8 1,581,092 - NIAGMN_08215 0.57 +1.1 1,581,113 - NIAGMN_08215 0.60 -1.5 1,581,148 + NIAGMN_08215 0.66 -0.3 1,581,181 + NIAGMN_08215 0.72 +0.8 1,581,242 - NIAGMN_08215 0.82 -0.9 1,581,251 + NIAGMN_08215 0.83 +0.8 1,581,255 + NIAGMN_08215 0.84 +0.3 1,581,256 - NIAGMN_08215 0.84 -2.8 1,581,260 - NIAGMN_08215 0.85 -0.6 1,581,260 - NIAGMN_08215 0.85 +0.4 1,581,269 + NIAGMN_08215 0.86 -0.5 1,581,313 - +0.4 1,581,329 + +0.4 1,581,330 - +1.1 1,581,341 + -0.8 1,581,341 + +0.7 1,581,342 - -0.3 1,581,342 - +0.6 1,581,343 + -2.0 1,581,343 + +0.7 1,581,343 + -0.7 1,581,343 + -0.2 1,581,368 + -0.3
Or see this region's nucleotide sequence