Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nei and pxpA are separated by 35 nucleotides pxpA and pxpC overlap by 11 nucleotides pxpC and pxpB overlap by 7 nucleotides pxpB and ybgI are separated by 22 nucleotides
NIAGMN_05045: nei - endonuclease VIII, at 917,668 to 918,459
nei
NIAGMN_05050: pxpA - 5-oxoprolinase subunit PxpA, at 918,495 to 919,229
pxpA
NIAGMN_05055: pxpC - 5-oxoprolinase subunit PxpC, at 919,219 to 920,151
pxpC
NIAGMN_05060: pxpB - 5-oxoprolinase subunit PxpB, at 920,145 to 920,801
pxpB
NIAGMN_05065: ybgI - radiation resistance protein YbgI, at 920,824 to 921,567
ybgI
Position (kb) 919
920
921 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 918.245 kb on + strand, within nei at 918.264 kb on - strand, within nei at 918.271 kb on + strand, within nei at 918.272 kb on - strand, within nei at 918.272 kb on - strand, within nei at 918.293 kb on - strand, within nei at 918.293 kb on - strand, within nei at 918.304 kb on - strand, within nei at 918.484 kb on - strand at 918.538 kb on - strand at 918.847 kb on + strand, within pxpA at 918.847 kb on + strand, within pxpA at 918.848 kb on - strand, within pxpA at 918.848 kb on - strand, within pxpA at 918.901 kb on + strand, within pxpA at 918.902 kb on - strand, within pxpA at 918.902 kb on - strand, within pxpA at 918.902 kb on - strand, within pxpA at 918.904 kb on - strand, within pxpA at 918.904 kb on - strand, within pxpA at 918.934 kb on - strand, within pxpA at 918.980 kb on - strand, within pxpA at 919.149 kb on - strand, within pxpA at 919.174 kb on - strand at 919.260 kb on - strand at 919.260 kb on - strand at 919.352 kb on + strand, within pxpC at 919.356 kb on + strand, within pxpC at 919.357 kb on - strand, within pxpC at 919.372 kb on - strand, within pxpC at 919.372 kb on - strand, within pxpC at 919.552 kb on - strand, within pxpC at 919.608 kb on - strand, within pxpC at 919.798 kb on - strand, within pxpC at 919.798 kb on - strand, within pxpC at 919.818 kb on - strand, within pxpC at 919.903 kb on - strand, within pxpC at 920.032 kb on - strand, within pxpC at 920.032 kb on - strand, within pxpC at 920.042 kb on - strand, within pxpC at 920.121 kb on + strand at 920.122 kb on - strand at 920.122 kb on - strand at 920.123 kb on + strand at 920.123 kb on + strand at 920.123 kb on + strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.124 kb on - strand at 920.151 kb on + strand at 920.196 kb on + strand at 920.196 kb on + strand at 920.197 kb on - strand at 920.197 kb on - strand at 920.199 kb on + strand at 920.200 kb on - strand at 920.200 kb on - strand at 920.200 kb on - strand at 920.200 kb on - strand at 920.243 kb on + strand, within pxpB at 920.364 kb on + strand, within pxpB at 920.364 kb on + strand, within pxpB at 920.365 kb on - strand, within pxpB at 920.365 kb on - strand, within pxpB at 920.390 kb on - strand, within pxpB at 920.428 kb on + strand, within pxpB at 920.428 kb on + strand, within pxpB at 920.429 kb on - strand, within pxpB at 920.429 kb on - strand, within pxpB at 920.541 kb on - strand, within pxpB at 920.776 kb on - strand at 920.847 kb on + strand at 920.848 kb on - strand at 920.902 kb on - strand, within ybgI at 920.902 kb on - strand, within ybgI at 920.997 kb on + strand, within ybgI at 921.034 kb on - strand, within ybgI at 921.112 kb on - strand, within ybgI at 921.112 kb on - strand, within ybgI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 918,245 + nei NIAGMN_05045 0.73 +1.0 918,264 - nei NIAGMN_05045 0.75 -0.7 918,271 + nei NIAGMN_05045 0.76 -0.5 918,272 - nei NIAGMN_05045 0.76 +0.4 918,272 - nei NIAGMN_05045 0.76 +1.2 918,293 - nei NIAGMN_05045 0.79 +1.3 918,293 - nei NIAGMN_05045 0.79 +0.7 918,304 - nei NIAGMN_05045 0.80 -1.8 918,484 - -3.4 918,538 - -0.7 918,847 + pxpA NIAGMN_05050 0.48 +0.7 918,847 + pxpA NIAGMN_05050 0.48 +0.0 918,848 - pxpA NIAGMN_05050 0.48 +0.1 918,848 - pxpA NIAGMN_05050 0.48 +2.1 918,901 + pxpA NIAGMN_05050 0.55 +1.9 918,902 - pxpA NIAGMN_05050 0.55 -0.5 918,902 - pxpA NIAGMN_05050 0.55 +1.9 918,902 - pxpA NIAGMN_05050 0.55 +0.8 918,904 - pxpA NIAGMN_05050 0.56 -1.8 918,904 - pxpA NIAGMN_05050 0.56 -1.3 918,934 - pxpA NIAGMN_05050 0.60 +0.3 918,980 - pxpA NIAGMN_05050 0.66 -0.8 919,149 - pxpA NIAGMN_05050 0.89 +0.6 919,174 - +1.9 919,260 - +0.5 919,260 - -0.9 919,352 + pxpC NIAGMN_05055 0.14 -2.9 919,356 + pxpC NIAGMN_05055 0.15 +0.6 919,357 - pxpC NIAGMN_05055 0.15 -3.6 919,372 - pxpC NIAGMN_05055 0.16 -2.4 919,372 - pxpC NIAGMN_05055 0.16 +1.5 919,552 - pxpC NIAGMN_05055 0.36 +0.8 919,608 - pxpC NIAGMN_05055 0.42 +0.0 919,798 - pxpC NIAGMN_05055 0.62 +2.2 919,798 - pxpC NIAGMN_05055 0.62 +0.3 919,818 - pxpC NIAGMN_05055 0.64 +0.4 919,903 - pxpC NIAGMN_05055 0.73 +0.6 920,032 - pxpC NIAGMN_05055 0.87 -0.7 920,032 - pxpC NIAGMN_05055 0.87 -1.2 920,042 - pxpC NIAGMN_05055 0.88 -2.3 920,121 + +1.8 920,122 - +0.5 920,122 - +0.9 920,123 + +0.6 920,123 + -0.3 920,123 + +0.8 920,124 - +1.2 920,124 - -0.7 920,124 - -0.4 920,124 - +2.3 920,124 - -0.4 920,124 - -0.6 920,124 - -0.8 920,151 + -1.5 920,196 + +1.8 920,196 + -2.4 920,197 - +0.5 920,197 - +1.6 920,199 + +2.2 920,200 - +1.2 920,200 - -0.9 920,200 - -1.2 920,200 - +0.3 920,243 + pxpB NIAGMN_05060 0.15 -2.9 920,364 + pxpB NIAGMN_05060 0.33 +2.3 920,364 + pxpB NIAGMN_05060 0.33 -2.5 920,365 - pxpB NIAGMN_05060 0.33 -1.7 920,365 - pxpB NIAGMN_05060 0.33 +2.4 920,390 - pxpB NIAGMN_05060 0.37 -0.1 920,428 + pxpB NIAGMN_05060 0.43 -3.8 920,428 + pxpB NIAGMN_05060 0.43 +0.2 920,429 - pxpB NIAGMN_05060 0.43 -0.1 920,429 - pxpB NIAGMN_05060 0.43 -1.7 920,541 - pxpB NIAGMN_05060 0.60 +0.2 920,776 - -1.4 920,847 + -0.6 920,848 - -0.9 920,902 - ybgI NIAGMN_05065 0.10 +0.7 920,902 - ybgI NIAGMN_05065 0.10 -0.3 920,997 + ybgI NIAGMN_05065 0.23 +0.8 921,034 - ybgI NIAGMN_05065 0.28 +1.0 921,112 - ybgI NIAGMN_05065 0.39 -0.5 921,112 - ybgI NIAGMN_05065 0.39 +0.7
Or see this region's nucleotide sequence