Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rimK and nfsA are separated by 60 nucleotides nfsA and NIAGMN_04025 overlap by 17 nucleotides NIAGMN_04025 and grxA are separated by 159 nucleotides grxA and ybjM are separated by 29 nucleotides
NIAGMN_04015: rimK - 30S ribosomal protein S6--L-glutamate ligase, at 722,884 to 723,786
rimK
NIAGMN_04020: nfsA - nitroreductase NfsA, at 723,847 to 724,569
nfsA
NIAGMN_04025: NIAGMN_04025 - DUF1418 domain-containing protein, at 724,553 to 724,840
_04025
NIAGMN_04030: grxA - glutaredoxin 1, at 725,000 to 725,257
grxA
NIAGMN_04035: ybjM - Inner membrane protein YbjM, at 725,287 to 725,664
ybjM
Position (kb) 723
724
725 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 722.871 kb on - strand at 722.906 kb on + strand at 722.917 kb on - strand at 723.086 kb on - strand, within rimK at 723.249 kb on + strand, within rimK at 723.251 kb on + strand, within rimK at 723.251 kb on + strand, within rimK at 723.401 kb on - strand, within rimK at 723.411 kb on + strand, within rimK at 723.572 kb on + strand, within rimK at 723.573 kb on - strand, within rimK at 723.579 kb on + strand, within rimK at 723.639 kb on - strand, within rimK at 723.665 kb on - strand, within rimK at 723.675 kb on - strand, within rimK at 723.675 kb on - strand, within rimK at 723.718 kb on + strand at 723.750 kb on - strand at 723.824 kb on + strand at 723.825 kb on - strand at 723.825 kb on - strand at 723.830 kb on - strand at 723.879 kb on - strand at 723.889 kb on - strand at 723.889 kb on - strand at 723.972 kb on - strand, within nfsA at 724.164 kb on + strand, within nfsA at 724.168 kb on - strand, within nfsA at 724.185 kb on + strand, within nfsA at 724.186 kb on - strand, within nfsA at 724.186 kb on - strand, within nfsA at 724.187 kb on + strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.188 kb on - strand, within nfsA at 724.309 kb on + strand, within nfsA at 724.477 kb on - strand, within nfsA at 724.548 kb on + strand at 724.549 kb on - strand at 724.570 kb on + strand at 724.596 kb on + strand, within NIAGMN_04025 at 724.652 kb on - strand, within NIAGMN_04025 at 724.826 kb on - strand at 724.826 kb on - strand at 724.826 kb on - strand at 724.840 kb on + strand at 724.887 kb on + strand at 724.887 kb on + strand at 724.888 kb on - strand at 724.888 kb on - strand at 724.888 kb on - strand at 724.978 kb on + strand at 724.978 kb on + strand at 724.978 kb on + strand at 725.036 kb on + strand, within grxA at 725.036 kb on + strand, within grxA at 725.096 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.102 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.106 kb on + strand, within grxA at 725.110 kb on + strand, within grxA at 725.131 kb on + strand, within grxA at 725.142 kb on + strand, within grxA at 725.142 kb on + strand, within grxA at 725.166 kb on + strand, within grxA at 725.203 kb on + strand, within grxA at 725.329 kb on - strand, within ybjM at 725.375 kb on - strand, within ybjM at 725.377 kb on + strand, within ybjM at 725.378 kb on - strand, within ybjM at 725.433 kb on + strand, within ybjM at 725.433 kb on + strand, within ybjM at 725.434 kb on - strand, within ybjM at 725.434 kb on - strand, within ybjM
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 722,871 - +0.0 722,906 + +1.6 722,917 - -0.1 723,086 - rimK NIAGMN_04015 0.22 -0.6 723,249 + rimK NIAGMN_04015 0.40 -0.1 723,251 + rimK NIAGMN_04015 0.41 +1.1 723,251 + rimK NIAGMN_04015 0.41 +1.5 723,401 - rimK NIAGMN_04015 0.57 -1.3 723,411 + rimK NIAGMN_04015 0.58 +1.6 723,572 + rimK NIAGMN_04015 0.76 -0.9 723,573 - rimK NIAGMN_04015 0.76 +1.0 723,579 + rimK NIAGMN_04015 0.77 -2.5 723,639 - rimK NIAGMN_04015 0.84 +1.0 723,665 - rimK NIAGMN_04015 0.86 +0.4 723,675 - rimK NIAGMN_04015 0.88 -0.4 723,675 - rimK NIAGMN_04015 0.88 +0.4 723,718 + +1.1 723,750 - -0.8 723,824 + +1.5 723,825 - +0.6 723,825 - +0.2 723,830 - -1.3 723,879 - +0.9 723,889 - +1.0 723,889 - +0.4 723,972 - nfsA NIAGMN_04020 0.17 +0.8 724,164 + nfsA NIAGMN_04020 0.44 -0.9 724,168 - nfsA NIAGMN_04020 0.44 -0.3 724,185 + nfsA NIAGMN_04020 0.47 +1.3 724,186 - nfsA NIAGMN_04020 0.47 +1.0 724,186 - nfsA NIAGMN_04020 0.47 -0.5 724,187 + nfsA NIAGMN_04020 0.47 -0.3 724,188 - nfsA NIAGMN_04020 0.47 +0.6 724,188 - nfsA NIAGMN_04020 0.47 -1.6 724,188 - nfsA NIAGMN_04020 0.47 +1.3 724,188 - nfsA NIAGMN_04020 0.47 +2.0 724,188 - nfsA NIAGMN_04020 0.47 -1.8 724,188 - nfsA NIAGMN_04020 0.47 +0.9 724,309 + nfsA NIAGMN_04020 0.64 +0.2 724,477 - nfsA NIAGMN_04020 0.87 +0.2 724,548 + -1.7 724,549 - +0.7 724,570 + -1.7 724,596 + NIAGMN_04025 0.15 +0.2 724,652 - NIAGMN_04025 0.34 +0.6 724,826 - +0.0 724,826 - -0.9 724,826 - -0.3 724,840 + -0.4 724,887 + +3.3 724,887 + -0.4 724,888 - +0.4 724,888 - +0.9 724,888 - -0.4 724,978 + +1.9 724,978 + -0.2 724,978 + +0.7 725,036 + grxA NIAGMN_04030 0.14 -0.1 725,036 + grxA NIAGMN_04030 0.14 +1.2 725,096 + grxA NIAGMN_04030 0.37 -0.8 725,102 + grxA NIAGMN_04030 0.40 +0.4 725,102 + grxA NIAGMN_04030 0.40 -0.7 725,102 + grxA NIAGMN_04030 0.40 +0.8 725,106 + grxA NIAGMN_04030 0.41 -1.4 725,106 + grxA NIAGMN_04030 0.41 +1.6 725,106 + grxA NIAGMN_04030 0.41 -1.1 725,106 + grxA NIAGMN_04030 0.41 +0.5 725,110 + grxA NIAGMN_04030 0.43 +0.9 725,131 + grxA NIAGMN_04030 0.51 -0.4 725,142 + grxA NIAGMN_04030 0.55 -0.3 725,142 + grxA NIAGMN_04030 0.55 -0.4 725,166 + grxA NIAGMN_04030 0.64 -0.9 725,203 + grxA NIAGMN_04030 0.79 +1.2 725,329 - ybjM NIAGMN_04035 0.11 +2.3 725,375 - ybjM NIAGMN_04035 0.23 -1.4 725,377 + ybjM NIAGMN_04035 0.24 +1.0 725,378 - ybjM NIAGMN_04035 0.24 -0.7 725,433 + ybjM NIAGMN_04035 0.39 +1.5 725,433 + ybjM NIAGMN_04035 0.39 -0.5 725,434 - ybjM NIAGMN_04035 0.39 -3.0 725,434 - ybjM NIAGMN_04035 0.39 -1.0
Or see this region's nucleotide sequence