Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt artP and artI are separated by 17 nucleotides artI and artQ are separated by 6 nucleotides artQ and artM overlap by 1 nucleotides artM and NIAGMN_03950 overlap by 1 nucleotides NIAGMN_03950 and artJ are separated by 153 nucleotides
NIAGMN_03930: artP - arginine ABC transporter ATP-binding protein ArtP, at 708,709 to 709,437
artP
NIAGMN_03935: artI - Putative ABC transporter arginine-binding protein 2, at 709,455 to 710,186
artI
NIAGMN_03940: artQ - arginine ABC transporter permease ArtQ, at 710,193 to 710,909
artQ
NIAGMN_03945: artM - arginine ABC transporter permease ArtM, at 710,909 to 711,577
artM
NIAGMN_03950: NIAGMN_03950 - hypothetical protein, at 711,577 to 711,714
_03950
NIAGMN_03960: artJ - ABC transporter substrate-binding protein ArtJ, at 711,868 to 712,599
artJ
Position (kb) 710
711 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 709.193 kb on + strand, within artP at 709.281 kb on + strand, within artP at 709.284 kb on + strand, within artP at 709.345 kb on + strand, within artP at 709.345 kb on + strand, within artP at 709.345 kb on + strand, within artP at 709.346 kb on - strand, within artP at 709.347 kb on + strand, within artP at 709.367 kb on + strand at 709.367 kb on + strand at 709.416 kb on + strand at 709.493 kb on + strand at 709.546 kb on - strand, within artI at 709.619 kb on + strand, within artI at 709.767 kb on + strand, within artI at 709.806 kb on + strand, within artI at 709.806 kb on + strand, within artI at 709.806 kb on + strand, within artI at 709.807 kb on - strand, within artI at 709.911 kb on + strand, within artI at 709.966 kb on + strand, within artI at 709.966 kb on + strand, within artI at 709.987 kb on + strand, within artI at 710.151 kb on + strand at 710.163 kb on + strand at 710.184 kb on + strand at 710.391 kb on + strand, within artQ at 710.391 kb on + strand, within artQ at 710.456 kb on - strand, within artQ at 710.535 kb on + strand, within artQ at 710.739 kb on - strand, within artQ at 710.918 kb on + strand at 711.007 kb on + strand, within artM at 711.061 kb on + strand, within artM at 711.183 kb on + strand, within artM at 711.183 kb on + strand, within artM at 711.239 kb on + strand, within artM at 711.340 kb on + strand, within artM at 711.391 kb on + strand, within artM at 711.404 kb on + strand, within artM at 711.461 kb on + strand, within artM at 711.461 kb on + strand, within artM at 711.493 kb on + strand, within artM at 711.493 kb on + strand, within artM at 711.575 kb on + strand at 711.588 kb on + strand at 711.588 kb on + strand at 711.608 kb on + strand, within NIAGMN_03950 at 711.836 kb on + strand at 711.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 709,193 + artP NIAGMN_03930 0.66 +1.2 709,281 + artP NIAGMN_03930 0.78 -0.3 709,284 + artP NIAGMN_03930 0.79 +0.1 709,345 + artP NIAGMN_03930 0.87 +2.0 709,345 + artP NIAGMN_03930 0.87 -1.2 709,345 + artP NIAGMN_03930 0.87 -1.4 709,346 - artP NIAGMN_03930 0.87 +0.5 709,347 + artP NIAGMN_03930 0.88 +1.0 709,367 + -1.8 709,367 + -1.2 709,416 + +2.0 709,493 + +0.5 709,546 - artI NIAGMN_03935 0.12 +1.2 709,619 + artI NIAGMN_03935 0.22 -0.9 709,767 + artI NIAGMN_03935 0.43 +0.4 709,806 + artI NIAGMN_03935 0.48 +1.1 709,806 + artI NIAGMN_03935 0.48 -0.3 709,806 + artI NIAGMN_03935 0.48 +0.7 709,807 - artI NIAGMN_03935 0.48 +1.8 709,911 + artI NIAGMN_03935 0.62 +0.3 709,966 + artI NIAGMN_03935 0.70 -0.2 709,966 + artI NIAGMN_03935 0.70 +1.1 709,987 + artI NIAGMN_03935 0.73 -3.3 710,151 + +0.4 710,163 + +0.3 710,184 + -4.5 710,391 + artQ NIAGMN_03940 0.28 -1.6 710,391 + artQ NIAGMN_03940 0.28 -0.1 710,456 - artQ NIAGMN_03940 0.37 -0.5 710,535 + artQ NIAGMN_03940 0.48 -1.2 710,739 - artQ NIAGMN_03940 0.76 +2.6 710,918 + -0.9 711,007 + artM NIAGMN_03945 0.15 +0.5 711,061 + artM NIAGMN_03945 0.23 -0.3 711,183 + artM NIAGMN_03945 0.41 -1.1 711,183 + artM NIAGMN_03945 0.41 +1.1 711,239 + artM NIAGMN_03945 0.49 -1.1 711,340 + artM NIAGMN_03945 0.64 +0.6 711,391 + artM NIAGMN_03945 0.72 -0.1 711,404 + artM NIAGMN_03945 0.74 +1.2 711,461 + artM NIAGMN_03945 0.83 +2.0 711,461 + artM NIAGMN_03945 0.83 +0.5 711,493 + artM NIAGMN_03945 0.87 +1.8 711,493 + artM NIAGMN_03945 0.87 -0.0 711,575 + -0.4 711,588 + +1.0 711,588 + -2.2 711,608 + NIAGMN_03950 0.22 +1.2 711,836 + +0.4 711,837 - +2.0
Or see this region's nucleotide sequence