Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_03410 and NIAGMN_03415 are separated by 53 nucleotides NIAGMN_03415 and NIAGMN_03420 overlap by 119 nucleotides NIAGMN_03420 and NIAGMN_03425 are separated by 112 nucleotides NIAGMN_03425 and NIAGMN_03430 are separated by 47 nucleotides
NIAGMN_03410: NIAGMN_03410 - putative transposase of insertion sequence IS682, at 612,441 to 612,866
_03410
NIAGMN_03415: NIAGMN_03415 - Integrase catalytic domain-containing protein, at 612,920 to 613,168
_03415
NIAGMN_03420: NIAGMN_03420 - BfpM-like protein, at 613,050 to 613,550
_03420
NIAGMN_03425: NIAGMN_03425 - hypothetical protein, at 613,663 to 613,818
_03425
NIAGMN_03430: NIAGMN_03430 - hypothetical protein, at 613,866 to 614,510
_03430
Position (kb) 613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 612.690 kb on - strand, within NIAGMN_03410 at 612.761 kb on + strand, within NIAGMN_03410 at 612.804 kb on - strand, within NIAGMN_03410 at 612.870 kb on + strand at 612.870 kb on + strand at 612.871 kb on - strand at 612.929 kb on + strand at 612.929 kb on + strand at 613.551 kb on - strand at 613.657 kb on - strand at 613.665 kb on - strand at 613.841 kb on - strand at 613.881 kb on - strand at 613.903 kb on - strand at 614.394 kb on + strand, within NIAGMN_03430 at 614.429 kb on - strand, within NIAGMN_03430 at 614.484 kb on - strand at 614.511 kb on + strand at 614.546 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 612,690 - NIAGMN_03410 0.58 -0.5 612,761 + NIAGMN_03410 0.75 +0.2 612,804 - NIAGMN_03410 0.85 +1.5 612,870 + -0.3 612,870 + -0.9 612,871 - +0.4 612,929 + +2.1 612,929 + -1.9 613,551 - +0.4 613,657 - -1.8 613,665 - -1.7 613,841 - +1.4 613,881 - -1.9 613,903 - -3.2 614,394 + NIAGMN_03430 0.82 +1.7 614,429 - NIAGMN_03430 0.87 +0.0 614,484 - -0.1 614,511 + +0.9 614,546 - -1.4
Or see this region's nucleotide sequence