Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptrB and yebE are separated by 208 nucleotides yebE and NIAGMN_01690 are separated by 59 nucleotides NIAGMN_01690 and NIAGMN_01695 are separated by 36 nucleotides NIAGMN_01695 and yebF overlap by 87 nucleotides yebF and yebG are separated by 66 nucleotides
NIAGMN_01680: ptrB - oligopeptidase B, at 304,192 to 306,252
ptrB
NIAGMN_01685: yebE - Inner membrane protein YebE, at 306,461 to 307,120
yebE
NIAGMN_01690: NIAGMN_01690 - hypothetical protein, at 307,180 to 307,290
_01690
NIAGMN_01695: NIAGMN_01695 - hypothetical protein, at 307,327 to 307,533
_01695
NIAGMN_01700: yebF - Protein YebF, at 307,447 to 307,803
yebF
NIAGMN_01705: yebG - DNA damage-inducible protein YebG, at 307,870 to 308,160
yebG
Position (kb) 307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 306.282 kb on - strand at 306.284 kb on - strand at 306.284 kb on - strand at 306.349 kb on + strand at 306.350 kb on - strand at 307.145 kb on - strand at 307.159 kb on - strand at 307.183 kb on + strand at 307.265 kb on + strand, within NIAGMN_01690 at 307.267 kb on + strand, within NIAGMN_01690 at 307.461 kb on - strand at 307.519 kb on - strand at 307.525 kb on + strand at 307.781 kb on - strand at 307.816 kb on - strand at 308.061 kb on + strand, within yebG at 308.079 kb on - strand, within yebG at 308.137 kb on + strand at 308.141 kb on + strand at 308.142 kb on - strand at 308.142 kb on - strand at 308.142 kb on - strand at 308.146 kb on - strand at 308.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 306,282 - +0.7 306,284 - -1.3 306,284 - -0.6 306,349 + +1.2 306,350 - -1.6 307,145 - +0.5 307,159 - +0.6 307,183 + -0.1 307,265 + NIAGMN_01690 0.77 +1.1 307,267 + NIAGMN_01690 0.78 +0.7 307,461 - -2.2 307,519 - -0.1 307,525 + -0.1 307,781 - +1.2 307,816 - +0.9 308,061 + yebG NIAGMN_01705 0.66 +0.2 308,079 - yebG NIAGMN_01705 0.72 +0.7 308,137 + -3.0 308,141 + +2.2 308,142 - -1.2 308,142 - -2.6 308,142 - -2.9 308,146 - +0.2 308,146 - -1.0
Or see this region's nucleotide sequence