Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manY and manZ are separated by 12 nucleotides manZ and NIAGMN_01545 are separated by 54 nucleotides NIAGMN_01545 and NIAGMN_01550 are separated by 310 nucleotides NIAGMN_01550 and mntP overlap by 4 nucleotides mntP and rlmA overlap by 4 nucleotides
NIAGMN_01535: manY - PTS mannose transporter subunit IIC, at 280,493 to 281,293
manY
NIAGMN_01540: manZ - PTS mannose transporter subunit IID, at 281,306 to 282,157
manZ
NIAGMN_01545: NIAGMN_01545 - UPF0266 membrane protein YobD, at 282,212 to 282,670
_01545
NIAGMN_01550: NIAGMN_01550 - Membrane protein, at 282,981 to 283,103
_01550
NIAGMN_01555: mntP - manganese efflux pump MntP, at 283,100 to 283,666
mntP
NIAGMN_01560: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 283,663 to 284,472
rlmA
Position (kb) 282
283 Strain fitness (log2 ratio) -1
0
1
2 at 281.357 kb on - strand at 281.380 kb on + strand at 281.465 kb on + strand, within manZ at 281.614 kb on + strand, within manZ at 281.740 kb on - strand, within manZ at 281.807 kb on + strand, within manZ at 281.884 kb on + strand, within manZ at 281.993 kb on - strand, within manZ at 282.018 kb on + strand, within manZ at 282.034 kb on - strand, within manZ at 282.058 kb on + strand, within manZ at 282.166 kb on + strand at 282.166 kb on + strand at 282.980 kb on + strand at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.041 kb on + strand, within NIAGMN_01550 at 283.042 kb on - strand, within NIAGMN_01550 at 283.135 kb on + strand at 283.537 kb on + strand, within mntP at 283.537 kb on + strand, within mntP at 283.668 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 281,357 - +0.6 281,380 + -1.1 281,465 + manZ NIAGMN_01540 0.19 +0.1 281,614 + manZ NIAGMN_01540 0.36 -1.1 281,740 - manZ NIAGMN_01540 0.51 +1.0 281,807 + manZ NIAGMN_01540 0.59 +1.1 281,884 + manZ NIAGMN_01540 0.68 +2.2 281,993 - manZ NIAGMN_01540 0.81 +0.5 282,018 + manZ NIAGMN_01540 0.84 +1.6 282,034 - manZ NIAGMN_01540 0.85 +0.2 282,058 + manZ NIAGMN_01540 0.88 +1.6 282,166 + +0.6 282,166 + +1.4 282,980 + +0.4 283,041 + NIAGMN_01550 0.49 -0.3 283,041 + NIAGMN_01550 0.49 +0.7 283,041 + NIAGMN_01550 0.49 +0.7 283,041 + NIAGMN_01550 0.49 +0.2 283,042 - NIAGMN_01550 0.50 +0.7 283,135 + +1.7 283,537 + mntP NIAGMN_01555 0.77 +1.6 283,537 + mntP NIAGMN_01555 0.77 -0.7 283,668 - -0.6
Or see this region's nucleotide sequence