Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01520

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsdaA and pdeD are separated by 130 nucleotidespdeD and yoaE are separated by 3 nucleotides NIAGMN_01515: sdaA - L-serine ammonia-lyase, at 274,343 to 275,707 sdaA NIAGMN_01520: pdeD - putative cyclic di-GMP phosphodiesterase PdeD, at 275,838 to 277,436 pdeD NIAGMN_01525: yoaE - CNNM family cation transport protein YoaE, at 277,440 to 278,996 yoaE Position (kb) 275 276 277 278Strain fitness (log2 ratio) -2 -1 0 1 2at 274.965 kb on - strand, within sdaAat 274.999 kb on + strand, within sdaAat 275.029 kb on - strand, within sdaAat 275.240 kb on + strand, within sdaAat 275.277 kb on - strand, within sdaAat 275.320 kb on + strand, within sdaAat 275.862 kb on - strandat 275.869 kb on - strandat 276.008 kb on - strand, within pdeDat 276.012 kb on - strand, within pdeDat 276.017 kb on - strand, within pdeDat 276.446 kb on - strand, within pdeDat 276.729 kb on - strand, within pdeDat 276.737 kb on - strand, within pdeDat 277.057 kb on + strand, within pdeDat 277.177 kb on + strand, within pdeDat 277.311 kb on - strandat 277.323 kb on + strandat 277.442 kb on - strandat 277.524 kb on - strandat 277.537 kb on - strandat 277.782 kb on + strand, within yoaEat 277.783 kb on - strand, within yoaEat 277.954 kb on - strand, within yoaEat 278.068 kb on - strand, within yoaEat 278.068 kb on - strand, within yoaE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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274,965 - sdaA NIAGMN_01515 0.46 +1.0
274,999 + sdaA NIAGMN_01515 0.48 +0.9
275,029 - sdaA NIAGMN_01515 0.50 -0.4
275,240 + sdaA NIAGMN_01515 0.66 -1.7
275,277 - sdaA NIAGMN_01515 0.68 -0.7
275,320 + sdaA NIAGMN_01515 0.72 -1.2
275,862 - +0.6
275,869 - -1.8
276,008 - pdeD NIAGMN_01520 0.11 +1.8
276,012 - pdeD NIAGMN_01520 0.11 -1.6
276,017 - pdeD NIAGMN_01520 0.11 -2.2
276,446 - pdeD NIAGMN_01520 0.38 -0.2
276,729 - pdeD NIAGMN_01520 0.56 +0.4
276,737 - pdeD NIAGMN_01520 0.56 +0.9
277,057 + pdeD NIAGMN_01520 0.76 -0.6
277,177 + pdeD NIAGMN_01520 0.84 -2.1
277,311 - -0.0
277,323 + -0.5
277,442 - +2.0
277,524 - +0.3
277,537 - -0.8
277,782 + yoaE NIAGMN_01525 0.22 +0.9
277,783 - yoaE NIAGMN_01525 0.22 +1.5
277,954 - yoaE NIAGMN_01525 0.33 +0.9
278,068 - yoaE NIAGMN_01525 0.40 +0.1
278,068 - yoaE NIAGMN_01525 0.40 +2.0

Or see this region's nucleotide sequence