Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01140

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspy and astE are separated by 329 nucleotidesastE and astB overlap by 8 nucleotides NIAGMN_01135: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 200,224 to 200,709 spy NIAGMN_01140: astE - succinylglutamate desuccinylase, at 201,039 to 202,007 astE NIAGMN_01145: astB - N-succinylarginine dihydrolase, at 202,000 to 203,343 astB Position (kb) 201 202 203Strain fitness (log2 ratio) -2 -1 0 1at 200.096 kb on + strandat 200.295 kb on + strand, within spyat 200.367 kb on + strand, within spyat 201.038 kb on - strandat 201.038 kb on - strandat 201.206 kb on + strand, within astEat 201.704 kb on + strand, within astEat 202.436 kb on + strand, within astBat 202.436 kb on + strand, within astBat 202.440 kb on + strand, within astBat 202.440 kb on + strand, within astBat 202.867 kb on - strand, within astB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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200,096 + +0.5
200,295 + spy NIAGMN_01135 0.15 +0.3
200,367 + spy NIAGMN_01135 0.29 +0.2
201,038 - +0.4
201,038 - -1.7
201,206 + astE NIAGMN_01140 0.17 +0.7
201,704 + astE NIAGMN_01140 0.69 -0.0
202,436 + astB NIAGMN_01145 0.32 -2.0
202,436 + astB NIAGMN_01145 0.32 +1.5
202,440 + astB NIAGMN_01145 0.33 -0.2
202,440 + astB NIAGMN_01145 0.33 +0.3
202,867 - astB NIAGMN_01145 0.65 -1.8

Or see this region's nucleotide sequence