Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00650

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlhr and grxD are separated by 50 nucleotidesgrxD and mepH are separated by 334 nucleotides NIAGMN_00645: lhr - ATP-dependent helicase, at 104,384 to 109,000 lhr NIAGMN_00650: grxD - monothiol glutaredoxin 4, at 109,051 to 109,398 grxD NIAGMN_00655: mepH - peptidoglycan DD-endopeptidase MepH, at 109,733 to 110,548 mepH Position (kb) 109 110Strain fitness (log2 ratio) -2 -1 0 1 2at 108.206 kb on - strand, within lhrat 108.943 kb on + strandat 109.080 kb on - strandat 109.080 kb on - strandat 109.128 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.201 kb on - strand, within grxDat 109.206 kb on - strand, within grxDat 109.253 kb on + strand, within grxDat 109.423 kb on - strandat 109.498 kb on - strandat 109.668 kb on - strandat 110.170 kb on + strand, within mepHat 110.341 kb on + strand, within mepH

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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108,206 - lhr NIAGMN_00645 0.83 +1.7
108,943 + -2.0
109,080 - +2.6
109,080 - +1.2
109,128 - grxD NIAGMN_00650 0.22 -0.7
109,201 - grxD NIAGMN_00650 0.43 -0.2
109,201 - grxD NIAGMN_00650 0.43 -0.3
109,201 - grxD NIAGMN_00650 0.43 -1.4
109,201 - grxD NIAGMN_00650 0.43 -1.0
109,206 - grxD NIAGMN_00650 0.45 -1.9
109,253 + grxD NIAGMN_00650 0.58 -1.2
109,423 - -0.9
109,498 - -1.4
109,668 - +1.1
110,170 + mepH NIAGMN_00655 0.54 -0.6
110,341 + mepH NIAGMN_00655 0.75 -1.2

Or see this region's nucleotide sequence