Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_00485 and ydgJ are separated by 35 nucleotides ydgJ and blr are separated by 242 nucleotides blr and ydgT are separated by 272 nucleotides ydgT and ydgK are separated by 85 nucleotides
NIAGMN_00485: NIAGMN_00485 - hypothetical protein, at 77,262 to 78,434
_00485
NIAGMN_00490: ydgJ - Uncharacterized oxidoreductase YdgJ, at 78,470 to 79,510
ydgJ
NIAGMN_00495: blr - division septum protein Blr, at 79,753 to 79,878
blr
NIAGMN_00500: ydgT - transcription modulator YdgT, at 80,151 to 80,366
ydgT
NIAGMN_00505: ydgK - Inner membrane protein YdgK, at 80,452 to 80,892
ydgK
Position (kb) 78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 77.660 kb on + strand, within NIAGMN_00485 at 78.303 kb on + strand, within NIAGMN_00485 at 78.603 kb on + strand, within ydgJ at 78.604 kb on - strand, within ydgJ at 78.604 kb on - strand, within ydgJ at 78.604 kb on - strand, within ydgJ at 78.678 kb on - strand, within ydgJ at 78.763 kb on - strand, within ydgJ at 78.797 kb on - strand, within ydgJ at 78.799 kb on - strand, within ydgJ at 78.799 kb on - strand, within ydgJ at 78.920 kb on + strand, within ydgJ at 78.948 kb on - strand, within ydgJ at 79.531 kb on + strand at 79.532 kb on - strand at 79.541 kb on - strand at 79.837 kb on + strand, within blr at 79.857 kb on + strand, within blr at 79.857 kb on + strand, within blr at 79.860 kb on + strand, within blr at 79.860 kb on + strand, within blr at 79.876 kb on + strand at 79.876 kb on + strand at 79.877 kb on - strand at 80.056 kb on + strand at 80.110 kb on - strand at 80.166 kb on + strand at 80.228 kb on + strand, within ydgT at 80.264 kb on + strand, within ydgT at 80.397 kb on + strand at 80.400 kb on + strand at 80.451 kb on + strand at 80.451 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 77,660 + NIAGMN_00485 0.34 -1.8 78,303 + NIAGMN_00485 0.89 -2.7 78,603 + ydgJ NIAGMN_00490 0.13 -0.6 78,604 - ydgJ NIAGMN_00490 0.13 +0.5 78,604 - ydgJ NIAGMN_00490 0.13 -0.7 78,604 - ydgJ NIAGMN_00490 0.13 -1.3 78,678 - ydgJ NIAGMN_00490 0.20 -2.5 78,763 - ydgJ NIAGMN_00490 0.28 -0.0 78,797 - ydgJ NIAGMN_00490 0.31 +0.3 78,799 - ydgJ NIAGMN_00490 0.32 +0.9 78,799 - ydgJ NIAGMN_00490 0.32 -1.4 78,920 + ydgJ NIAGMN_00490 0.43 -0.3 78,948 - ydgJ NIAGMN_00490 0.46 -2.1 79,531 + +1.1 79,532 - +1.2 79,541 - +2.7 79,837 + blr NIAGMN_00495 0.67 -1.2 79,857 + blr NIAGMN_00495 0.83 -1.4 79,857 + blr NIAGMN_00495 0.83 +1.4 79,860 + blr NIAGMN_00495 0.85 +0.8 79,860 + blr NIAGMN_00495 0.85 -0.1 79,876 + +0.6 79,876 + +1.2 79,877 - +1.0 80,056 + +0.1 80,110 - -2.8 80,166 + +0.3 80,228 + ydgT NIAGMN_00500 0.36 +0.6 80,264 + ydgT NIAGMN_00500 0.52 -1.4 80,397 + +1.8 80,400 + +3.4 80,451 + +0.8 80,451 + -1.5
Or see this region's nucleotide sequence