Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00425

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttus and fumC overlap by 4 nucleotidesfumC and fumB are separated by 142 nucleotides NIAGMN_00420: tus - DNA replication terminus site-binding protein, at 62,338 to 63,267 tus NIAGMN_00425: fumC - class II fumarate hydratase, at 63,264 to 64,667 fumC NIAGMN_00430: fumB - class I fumarate hydratase, at 64,810 to 66,456 fumB Position (kb) 63 64 65Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 62.303 kb on - strandat 62.334 kb on + strandat 62.865 kb on + strand, within tusat 62.871 kb on + strand, within tusat 63.067 kb on + strand, within tusat 63.204 kb on + strandat 63.466 kb on - strand, within fumCat 64.019 kb on - strand, within fumCat 64.656 kb on + strandat 64.661 kb on + strandat 64.812 kb on - strandat 64.844 kb on - strandat 64.844 kb on - strandat 64.966 kb on - strandat 64.966 kb on - strandat 64.966 kb on - strandat 65.165 kb on + strand, within fumBat 65.436 kb on + strand, within fumB

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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62,303 - -0.4
62,334 + -1.0
62,865 + tus NIAGMN_00420 0.57 -0.9
62,871 + tus NIAGMN_00420 0.57 +0.8
63,067 + tus NIAGMN_00420 0.78 +1.0
63,204 + +0.9
63,466 - fumC NIAGMN_00425 0.14 +0.1
64,019 - fumC NIAGMN_00425 0.54 +0.6
64,656 + -1.2
64,661 + +2.8
64,812 - -0.8
64,844 - +0.2
64,844 - -1.5
64,966 - -1.7
64,966 - -0.9
64,966 - -3.3
65,165 + fumB NIAGMN_00430 0.22 -2.5
65,436 + fumB NIAGMN_00430 0.38 +1.2

Or see this region's nucleotide sequence