Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpB and insE overlap by 4 nucleotides insE and rpmE2-1 are separated by 260 nucleotides rpmE2-1 and MCAODC_28410 overlap by 1 nucleotides MCAODC_28410 and MCAODC_28415 are separated by 69 nucleotides
MCAODC_28395: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 2,434,297 to 2,434,644
tnpB
MCAODC_28400: insE - Transposase InsE and inactivated derivatives, at 2,434,641 to 2,435,021
insE
MCAODC_28405: rpmE2-1 - type B 50S ribosomal protein L31, at 2,435,282 to 2,435,548
rpmE2-1
MCAODC_28410: MCAODC_28410 - 50S ribosomal protein L36 2, at 2,435,548 to 2,435,688
_28410
MCAODC_28415: MCAODC_28415 - Protein, at 2,435,758 to 2,435,949
_28415
Position (kb)
2435
2436 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2435.105 kb on + strand at 2435.195 kb on + strand at 2435.315 kb on - strand, within rpmE2-1 at 2435.315 kb on - strand, within rpmE2-1 at 2435.315 kb on - strand, within rpmE2-1 at 2435.315 kb on - strand, within rpmE2-1 at 2435.315 kb on - strand, within rpmE2-1 at 2435.349 kb on - strand, within rpmE2-1 at 2435.349 kb on - strand, within rpmE2-1 at 2435.349 kb on - strand, within rpmE2-1 at 2435.354 kb on - strand, within rpmE2-1 at 2435.408 kb on + strand, within rpmE2-1 at 2435.408 kb on + strand, within rpmE2-1 at 2435.409 kb on - strand, within rpmE2-1 at 2435.415 kb on - strand, within rpmE2-1 at 2435.418 kb on + strand, within rpmE2-1 at 2435.419 kb on - strand, within rpmE2-1 at 2435.419 kb on - strand, within rpmE2-1 at 2435.453 kb on + strand, within rpmE2-1 at 2435.454 kb on - strand, within rpmE2-1 at 2435.454 kb on - strand, within rpmE2-1 at 2435.456 kb on - strand, within rpmE2-1 at 2435.559 kb on + strand at 2435.560 kb on - strand at 2435.624 kb on + strand, within MCAODC_28410 at 2435.624 kb on + strand, within MCAODC_28410 at 2435.625 kb on - strand, within MCAODC_28410 at 2435.625 kb on - strand, within MCAODC_28410 at 2435.626 kb on + strand, within MCAODC_28410 at 2435.626 kb on + strand, within MCAODC_28410 at 2435.626 kb on + strand, within MCAODC_28410 at 2435.627 kb on - strand, within MCAODC_28410 at 2435.627 kb on - strand, within MCAODC_28410 at 2435.627 kb on - strand, within MCAODC_28410 at 2435.644 kb on - strand, within MCAODC_28410 at 2435.712 kb on + strand at 2435.712 kb on + strand at 2435.712 kb on + strand at 2435.712 kb on + strand at 2435.752 kb on + strand at 2435.752 kb on + strand at 2435.756 kb on + strand at 2435.788 kb on - strand, within MCAODC_28415 at 2435.788 kb on - strand, within MCAODC_28415 at 2435.788 kb on - strand, within MCAODC_28415 at 2435.788 kb on - strand, within MCAODC_28415 at 2435.788 kb on - strand, within MCAODC_28415 at 2435.804 kb on - strand, within MCAODC_28415 at 2435.804 kb on - strand, within MCAODC_28415 at 2435.834 kb on + strand, within MCAODC_28415 at 2435.842 kb on + strand, within MCAODC_28415 at 2435.847 kb on + strand, within MCAODC_28415 at 2435.848 kb on - strand, within MCAODC_28415 at 2435.848 kb on - strand, within MCAODC_28415 at 2435.880 kb on - strand, within MCAODC_28415 at 2435.901 kb on + strand, within MCAODC_28415 at 2435.902 kb on - strand, within MCAODC_28415 at 2435.951 kb on + strand at 2435.952 kb on - strand at 2435.973 kb on + strand at 2435.973 kb on + strand at 2435.974 kb on - strand at 2435.983 kb on - strand at 2435.994 kb on + strand at 2435.994 kb on + strand at 2436.003 kb on - strand at 2436.003 kb on - strand at 2436.071 kb on + strand at 2436.071 kb on + strand at 2436.071 kb on + strand at 2436.174 kb on - strand at 2436.199 kb on - strand at 2436.202 kb on + strand at 2436.267 kb on - strand at 2436.419 kb on + strand at 2436.423 kb on + strand at 2436.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,435,105 + +0.6 2,435,195 + +0.6 2,435,315 - rpmE2-1 MCAODC_28405 0.12 -1.6 2,435,315 - rpmE2-1 MCAODC_28405 0.12 +0.2 2,435,315 - rpmE2-1 MCAODC_28405 0.12 -0.5 2,435,315 - rpmE2-1 MCAODC_28405 0.12 +0.5 2,435,315 - rpmE2-1 MCAODC_28405 0.12 -3.5 2,435,349 - rpmE2-1 MCAODC_28405 0.25 -0.1 2,435,349 - rpmE2-1 MCAODC_28405 0.25 -1.6 2,435,349 - rpmE2-1 MCAODC_28405 0.25 +0.5 2,435,354 - rpmE2-1 MCAODC_28405 0.27 +0.4 2,435,408 + rpmE2-1 MCAODC_28405 0.47 -0.1 2,435,408 + rpmE2-1 MCAODC_28405 0.47 +0.0 2,435,409 - rpmE2-1 MCAODC_28405 0.48 -2.4 2,435,415 - rpmE2-1 MCAODC_28405 0.50 +0.3 2,435,418 + rpmE2-1 MCAODC_28405 0.51 +1.8 2,435,419 - rpmE2-1 MCAODC_28405 0.51 -0.3 2,435,419 - rpmE2-1 MCAODC_28405 0.51 -0.2 2,435,453 + rpmE2-1 MCAODC_28405 0.64 -2.1 2,435,454 - rpmE2-1 MCAODC_28405 0.64 -0.4 2,435,454 - rpmE2-1 MCAODC_28405 0.64 +0.6 2,435,456 - rpmE2-1 MCAODC_28405 0.65 +0.1 2,435,559 + -1.3 2,435,560 - +0.9 2,435,624 + MCAODC_28410 0.54 -1.4 2,435,624 + MCAODC_28410 0.54 -3.4 2,435,625 - MCAODC_28410 0.55 -0.3 2,435,625 - MCAODC_28410 0.55 -4.3 2,435,626 + MCAODC_28410 0.55 +2.0 2,435,626 + MCAODC_28410 0.55 -2.0 2,435,626 + MCAODC_28410 0.55 +1.3 2,435,627 - MCAODC_28410 0.56 +0.4 2,435,627 - MCAODC_28410 0.56 +2.1 2,435,627 - MCAODC_28410 0.56 -0.6 2,435,644 - MCAODC_28410 0.68 +0.9 2,435,712 + -1.8 2,435,712 + +0.1 2,435,712 + +0.7 2,435,712 + -1.1 2,435,752 + +0.7 2,435,752 + -3.8 2,435,756 + -0.6 2,435,788 - MCAODC_28415 0.16 -3.4 2,435,788 - MCAODC_28415 0.16 -1.7 2,435,788 - MCAODC_28415 0.16 +0.5 2,435,788 - MCAODC_28415 0.16 +1.4 2,435,788 - MCAODC_28415 0.16 -2.2 2,435,804 - MCAODC_28415 0.24 +1.5 2,435,804 - MCAODC_28415 0.24 +1.9 2,435,834 + MCAODC_28415 0.40 +0.2 2,435,842 + MCAODC_28415 0.44 -4.3 2,435,847 + MCAODC_28415 0.46 -3.3 2,435,848 - MCAODC_28415 0.47 -2.1 2,435,848 - MCAODC_28415 0.47 +1.2 2,435,880 - MCAODC_28415 0.64 +0.4 2,435,901 + MCAODC_28415 0.74 +0.6 2,435,902 - MCAODC_28415 0.75 +1.0 2,435,951 + +0.7 2,435,952 - -2.7 2,435,973 + +1.2 2,435,973 + -3.4 2,435,974 - +2.0 2,435,983 - -0.5 2,435,994 + +0.9 2,435,994 + +1.7 2,436,003 - +0.5 2,436,003 - +2.7 2,436,071 + +1.1 2,436,071 + +1.6 2,436,071 + -0.3 2,436,174 - -0.4 2,436,199 - -2.9 2,436,202 + -1.3 2,436,267 - -0.6 2,436,419 + -0.4 2,436,423 + -0.7 2,436,429 - +0.5
Or see this region's nucleotide sequence