Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_24550 and Ser_trna are separated by 111 nucleotides Ser_trna and yccA are separated by 206 nucleotides yccA and tusE are separated by 90 nucleotides tusE and yccX overlap by 4 nucleotides yccX and rlmI are separated by 109 nucleotides
MCAODC_24550: MCAODC_24550 - Integrase from prophage, at 1,618,838 to 1,619,416
_24550
MCAODC_24555: Ser_trna - tRNA-Ser(tga), at 1,619,528 to 1,619,617
_trna
MCAODC_24560: yccA - FtsH protease modulator YccA, at 1,619,824 to 1,620,483
yccA
MCAODC_24565: tusE - sulfurtransferase TusE, at 1,620,574 to 1,620,903
tusE
MCAODC_24570: yccX - acylphosphatase, at 1,620,900 to 1,621,178
yccX
MCAODC_24575: rlmI - 23S rRNA (cytosine(1962)-C(5))-methyltransferase RlmI, at 1,621,288 to 1,622,463
rlmI
Position (kb)
1619
1620
1621 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1618.835 kb on + strand at 1618.835 kb on + strand at 1618.835 kb on + strand at 1618.836 kb on - strand at 1618.879 kb on + strand at 1618.895 kb on + strand at 1618.895 kb on + strand at 1618.913 kb on + strand, within MCAODC_24550 at 1618.923 kb on + strand, within MCAODC_24550 at 1618.923 kb on + strand, within MCAODC_24550 at 1618.923 kb on + strand, within MCAODC_24550 at 1618.923 kb on + strand, within MCAODC_24550 at 1618.924 kb on - strand, within MCAODC_24550 at 1618.925 kb on + strand, within MCAODC_24550 at 1618.925 kb on + strand, within MCAODC_24550 at 1618.925 kb on + strand, within MCAODC_24550 at 1618.925 kb on + strand, within MCAODC_24550 at 1618.926 kb on - strand, within MCAODC_24550 at 1618.947 kb on + strand, within MCAODC_24550 at 1618.947 kb on + strand, within MCAODC_24550 at 1618.947 kb on + strand, within MCAODC_24550 at 1619.020 kb on + strand, within MCAODC_24550 at 1619.021 kb on - strand, within MCAODC_24550 at 1619.029 kb on + strand, within MCAODC_24550 at 1619.030 kb on - strand, within MCAODC_24550 at 1619.039 kb on + strand, within MCAODC_24550 at 1619.039 kb on + strand, within MCAODC_24550 at 1619.039 kb on + strand, within MCAODC_24550 at 1619.039 kb on + strand, within MCAODC_24550 at 1619.039 kb on + strand, within MCAODC_24550 at 1619.040 kb on - strand, within MCAODC_24550 at 1619.094 kb on + strand, within MCAODC_24550 at 1619.098 kb on + strand, within MCAODC_24550 at 1619.098 kb on + strand, within MCAODC_24550 at 1619.098 kb on + strand, within MCAODC_24550 at 1619.098 kb on + strand, within MCAODC_24550 at 1619.098 kb on + strand, within MCAODC_24550 at 1619.099 kb on - strand, within MCAODC_24550 at 1619.112 kb on + strand, within MCAODC_24550 at 1619.124 kb on + strand, within MCAODC_24550 at 1619.124 kb on + strand, within MCAODC_24550 at 1619.125 kb on - strand, within MCAODC_24550 at 1619.125 kb on - strand, within MCAODC_24550 at 1619.134 kb on + strand, within MCAODC_24550 at 1619.155 kb on + strand, within MCAODC_24550 at 1619.156 kb on - strand, within MCAODC_24550 at 1619.182 kb on + strand, within MCAODC_24550 at 1619.194 kb on + strand, within MCAODC_24550 at 1619.268 kb on + strand, within MCAODC_24550 at 1619.269 kb on - strand, within MCAODC_24550 at 1619.291 kb on + strand, within MCAODC_24550 at 1619.332 kb on + strand, within MCAODC_24550 at 1619.358 kb on - strand, within MCAODC_24550 at 1619.414 kb on + strand at 1619.451 kb on + strand at 1619.452 kb on - strand at 1619.452 kb on - strand at 1619.453 kb on + strand at 1619.456 kb on + strand at 1619.457 kb on - strand at 1619.457 kb on - strand at 1619.474 kb on + strand at 1619.474 kb on + strand at 1619.478 kb on - strand at 1619.620 kb on + strand at 1619.620 kb on + strand at 1619.625 kb on + strand at 1619.626 kb on - strand at 1619.633 kb on + strand at 1619.633 kb on + strand at 1619.637 kb on + strand at 1619.684 kb on + strand at 1619.685 kb on - strand at 1619.685 kb on - strand at 1619.686 kb on + strand at 1619.686 kb on + strand at 1619.686 kb on + strand at 1619.686 kb on + strand at 1619.687 kb on - strand at 1619.694 kb on + strand at 1619.694 kb on + strand at 1619.705 kb on + strand at 1619.706 kb on - strand at 1619.706 kb on - strand at 1619.741 kb on - strand at 1619.750 kb on + strand at 1619.756 kb on + strand at 1619.783 kb on - strand at 1619.791 kb on + strand at 1619.792 kb on - strand at 1619.792 kb on - strand at 1619.800 kb on - strand at 1619.810 kb on + strand at 1619.832 kb on + strand at 1619.838 kb on + strand at 1619.856 kb on + strand at 1619.856 kb on + strand at 1619.868 kb on + strand at 1619.877 kb on + strand at 1619.896 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.001 kb on + strand, within yccA at 1620.002 kb on - strand, within yccA at 1620.088 kb on - strand, within yccA at 1620.112 kb on + strand, within yccA at 1620.112 kb on + strand, within yccA at 1620.167 kb on + strand, within yccA at 1620.190 kb on + strand, within yccA at 1620.213 kb on + strand, within yccA at 1620.231 kb on + strand, within yccA at 1620.267 kb on + strand, within yccA at 1620.267 kb on + strand, within yccA at 1620.276 kb on + strand, within yccA at 1620.277 kb on - strand, within yccA at 1620.277 kb on - strand, within yccA at 1620.349 kb on - strand, within yccA at 1620.394 kb on + strand, within yccA at 1620.394 kb on + strand, within yccA at 1620.394 kb on + strand, within yccA at 1620.394 kb on + strand, within yccA at 1620.396 kb on + strand, within yccA at 1620.418 kb on + strand at 1620.418 kb on + strand at 1620.431 kb on - strand at 1620.506 kb on - strand at 1620.558 kb on + strand at 1620.563 kb on + strand at 1620.569 kb on + strand at 1620.573 kb on + strand at 1620.994 kb on - strand, within yccX at 1621.015 kb on - strand, within yccX at 1621.105 kb on + strand, within yccX at 1621.105 kb on + strand, within yccX at 1621.106 kb on - strand, within yccX at 1621.117 kb on + strand, within yccX at 1621.272 kb on + strand at 1621.280 kb on + strand at 1621.286 kb on + strand at 1621.368 kb on + strand at 1621.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,618,835 + -2.2 1,618,835 + -3.7 1,618,835 + +0.4 1,618,836 - -0.9 1,618,879 + +0.5 1,618,895 + -1.7 1,618,895 + -1.6 1,618,913 + MCAODC_24550 0.13 +0.5 1,618,923 + MCAODC_24550 0.15 -3.9 1,618,923 + MCAODC_24550 0.15 -0.0 1,618,923 + MCAODC_24550 0.15 -0.7 1,618,923 + MCAODC_24550 0.15 +1.4 1,618,924 - MCAODC_24550 0.15 -1.0 1,618,925 + MCAODC_24550 0.15 +0.8 1,618,925 + MCAODC_24550 0.15 +1.3 1,618,925 + MCAODC_24550 0.15 -0.9 1,618,925 + MCAODC_24550 0.15 +0.5 1,618,926 - MCAODC_24550 0.15 +0.6 1,618,947 + MCAODC_24550 0.19 -0.8 1,618,947 + MCAODC_24550 0.19 +0.4 1,618,947 + MCAODC_24550 0.19 -0.8 1,619,020 + MCAODC_24550 0.31 +0.1 1,619,021 - MCAODC_24550 0.32 +1.7 1,619,029 + MCAODC_24550 0.33 +0.6 1,619,030 - MCAODC_24550 0.33 -0.9 1,619,039 + MCAODC_24550 0.35 -0.6 1,619,039 + MCAODC_24550 0.35 -1.6 1,619,039 + MCAODC_24550 0.35 +0.1 1,619,039 + MCAODC_24550 0.35 -0.1 1,619,039 + MCAODC_24550 0.35 -1.7 1,619,040 - MCAODC_24550 0.35 -3.4 1,619,094 + MCAODC_24550 0.44 +0.7 1,619,098 + MCAODC_24550 0.45 +1.0 1,619,098 + MCAODC_24550 0.45 -1.8 1,619,098 + MCAODC_24550 0.45 +3.0 1,619,098 + MCAODC_24550 0.45 +0.7 1,619,098 + MCAODC_24550 0.45 +0.9 1,619,099 - MCAODC_24550 0.45 +1.8 1,619,112 + MCAODC_24550 0.47 -1.4 1,619,124 + MCAODC_24550 0.49 +1.0 1,619,124 + MCAODC_24550 0.49 +0.4 1,619,125 - MCAODC_24550 0.50 -2.7 1,619,125 - MCAODC_24550 0.50 +1.8 1,619,134 + MCAODC_24550 0.51 -1.8 1,619,155 + MCAODC_24550 0.55 +1.9 1,619,156 - MCAODC_24550 0.55 -0.4 1,619,182 + MCAODC_24550 0.59 +0.4 1,619,194 + MCAODC_24550 0.61 -3.0 1,619,268 + MCAODC_24550 0.74 +0.5 1,619,269 - MCAODC_24550 0.74 -1.8 1,619,291 + MCAODC_24550 0.78 +1.3 1,619,332 + MCAODC_24550 0.85 -2.7 1,619,358 - MCAODC_24550 0.90 +0.9 1,619,414 + -4.1 1,619,451 + +0.8 1,619,452 - +2.4 1,619,452 - +1.5 1,619,453 + -0.7 1,619,456 + +1.1 1,619,457 - -2.4 1,619,457 - +0.8 1,619,474 + +0.6 1,619,474 + +0.8 1,619,478 - -3.6 1,619,620 + -3.9 1,619,620 + -0.7 1,619,625 + +0.5 1,619,626 - +0.6 1,619,633 + -3.4 1,619,633 + +1.7 1,619,637 + +1.4 1,619,684 + -0.8 1,619,685 - +1.1 1,619,685 - -0.7 1,619,686 + -0.3 1,619,686 + -0.9 1,619,686 + +0.3 1,619,686 + +0.5 1,619,687 - +0.3 1,619,694 + -3.7 1,619,694 + +1.0 1,619,705 + -3.2 1,619,706 - -1.4 1,619,706 - +0.3 1,619,741 - -0.1 1,619,750 + -1.9 1,619,756 + -0.9 1,619,783 - +1.1 1,619,791 + +1.0 1,619,792 - +0.0 1,619,792 - -0.4 1,619,800 - +1.8 1,619,810 + -2.6 1,619,832 + +1.8 1,619,838 + +0.3 1,619,856 + -2.2 1,619,856 + -3.1 1,619,868 + +0.1 1,619,877 + -0.7 1,619,896 + yccA MCAODC_24560 0.11 -0.5 1,620,001 + yccA MCAODC_24560 0.27 +0.0 1,620,001 + yccA MCAODC_24560 0.27 +1.6 1,620,001 + yccA MCAODC_24560 0.27 -1.3 1,620,001 + yccA MCAODC_24560 0.27 +1.1 1,620,001 + yccA MCAODC_24560 0.27 +0.3 1,620,001 + yccA MCAODC_24560 0.27 -1.6 1,620,001 + yccA MCAODC_24560 0.27 -0.4 1,620,002 - yccA MCAODC_24560 0.27 -0.8 1,620,088 - yccA MCAODC_24560 0.40 +0.8 1,620,112 + yccA MCAODC_24560 0.44 +2.3 1,620,112 + yccA MCAODC_24560 0.44 -1.9 1,620,167 + yccA MCAODC_24560 0.52 +0.9 1,620,190 + yccA MCAODC_24560 0.55 -1.4 1,620,213 + yccA MCAODC_24560 0.59 +0.2 1,620,231 + yccA MCAODC_24560 0.62 +2.5 1,620,267 + yccA MCAODC_24560 0.67 +3.6 1,620,267 + yccA MCAODC_24560 0.67 -0.9 1,620,276 + yccA MCAODC_24560 0.68 -0.9 1,620,277 - yccA MCAODC_24560 0.69 +0.6 1,620,277 - yccA MCAODC_24560 0.69 -2.0 1,620,349 - yccA MCAODC_24560 0.80 +0.8 1,620,394 + yccA MCAODC_24560 0.86 -0.2 1,620,394 + yccA MCAODC_24560 0.86 -0.0 1,620,394 + yccA MCAODC_24560 0.86 -0.0 1,620,394 + yccA MCAODC_24560 0.86 +1.4 1,620,396 + yccA MCAODC_24560 0.87 +0.6 1,620,418 + -2.1 1,620,418 + -0.7 1,620,431 - -3.6 1,620,506 - -1.3 1,620,558 + +1.6 1,620,563 + +0.4 1,620,569 + -0.1 1,620,573 + -1.6 1,620,994 - yccX MCAODC_24570 0.34 -2.3 1,621,015 - yccX MCAODC_24570 0.41 +0.0 1,621,105 + yccX MCAODC_24570 0.73 -0.1 1,621,105 + yccX MCAODC_24570 0.73 +1.2 1,621,106 - yccX MCAODC_24570 0.74 +0.3 1,621,117 + yccX MCAODC_24570 0.78 -0.8 1,621,272 + +0.1 1,621,280 + -0.2 1,621,286 + -0.6 1,621,368 + -0.8 1,621,368 + +0.6
Or see this region's nucleotide sequence