Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eAL and MCAODC_23910 are separated by 97 nucleotides MCAODC_23910 and MCAODC_23915 overlap by 4 nucleotides MCAODC_23915 and pgaA are separated by 586 nucleotides
MCAODC_23905: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 1,504,210 to 1,505,820
eAL
MCAODC_23910: MCAODC_23910 - GGDEF domain-containing protein, at 1,505,918 to 1,506,334
_23910
MCAODC_23915: MCAODC_23915 - Putative diguanylate cyclase, at 1,506,331 to 1,507,275
_23915
MCAODC_23920: pgaA - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 1,507,862 to 1,509,619
pgaA
Position (kb)
1506
1507
1508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1505.616 kb on + strand, within eAL at 1505.690 kb on - strand at 1505.772 kb on - strand at 1505.797 kb on - strand at 1505.988 kb on + strand, within MCAODC_23910 at 1506.086 kb on - strand, within MCAODC_23910 at 1506.231 kb on - strand, within MCAODC_23910 at 1506.464 kb on - strand, within MCAODC_23915 at 1507.241 kb on - strand at 1507.328 kb on - strand at 1507.419 kb on + strand at 1507.453 kb on - strand at 1507.453 kb on - strand at 1507.607 kb on - strand at 1507.701 kb on + strand at 1507.703 kb on + strand at 1507.844 kb on + strand at 1507.844 kb on + strand at 1507.844 kb on + strand at 1507.856 kb on + strand at 1507.866 kb on - strand at 1507.866 kb on - strand at 1508.008 kb on + strand at 1508.105 kb on - strand, within pgaA at 1508.215 kb on + strand, within pgaA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,505,616 + eAL MCAODC_23905 0.87 -2.2 1,505,690 - +1.4 1,505,772 - +0.3 1,505,797 - +1.6 1,505,988 + MCAODC_23910 0.17 +0.1 1,506,086 - MCAODC_23910 0.40 +2.1 1,506,231 - MCAODC_23910 0.75 -3.7 1,506,464 - MCAODC_23915 0.14 +1.4 1,507,241 - -0.2 1,507,328 - -2.2 1,507,419 + -1.3 1,507,453 - +0.3 1,507,453 - -0.9 1,507,607 - +1.5 1,507,701 + +2.1 1,507,703 + +1.7 1,507,844 + -0.8 1,507,844 + -0.3 1,507,844 + -0.3 1,507,856 + +0.4 1,507,866 - +0.1 1,507,866 - -0.9 1,508,008 + +0.2 1,508,105 - pgaA MCAODC_23920 0.14 +0.2 1,508,215 + pgaA MCAODC_23920 0.20 -3.1
Or see this region's nucleotide sequence