Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cah and yeeP are separated by 371 nucleotides yeeP and MCAODC_23320 are separated by 327 nucleotides MCAODC_23320 and MCAODC_23325 are separated by 213 nucleotides MCAODC_23325 and MCAODC_23330 overlap by 4 nucleotides
MCAODC_23310: cah - calcium-binding autotransporter Cah, at 1,393,480 to 1,396,329
cah
MCAODC_23315: yeeP - 50S ribosome-binding GTPase, at 1,396,701 to 1,397,573
yeeP
MCAODC_23320: MCAODC_23320 - hypothetical protein, at 1,397,901 to 1,398,083
_23320
MCAODC_23325: MCAODC_23325 - IS66 transposase, at 1,398,297 to 1,398,386
_23325
MCAODC_23330: MCAODC_23330 - putative transposase of insertion sequence IS682, at 1,398,383 to 1,398,808
_23330
Position (kb)
1396
1397
1398 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1395.720 kb on + strand, within cah at 1395.722 kb on + strand, within cah at 1395.828 kb on + strand, within cah at 1396.160 kb on - strand at 1396.392 kb on + strand at 1396.393 kb on - strand at 1396.595 kb on - strand at 1396.674 kb on - strand at 1397.020 kb on - strand, within yeeP at 1397.157 kb on + strand, within yeeP at 1397.157 kb on + strand, within yeeP at 1397.158 kb on - strand, within yeeP at 1397.158 kb on - strand, within yeeP at 1397.173 kb on + strand, within yeeP at 1397.220 kb on + strand, within yeeP at 1397.220 kb on + strand, within yeeP at 1397.263 kb on + strand, within yeeP at 1397.450 kb on + strand, within yeeP at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.573 kb on + strand at 1397.574 kb on - strand at 1397.574 kb on - strand at 1397.579 kb on + strand at 1397.579 kb on + strand at 1397.580 kb on - strand at 1397.580 kb on - strand at 1397.674 kb on - strand at 1397.690 kb on - strand at 1397.690 kb on - strand at 1397.690 kb on - strand at 1397.713 kb on - strand at 1397.906 kb on + strand at 1397.939 kb on - strand, within MCAODC_23320 at 1398.303 kb on + strand at 1398.303 kb on + strand at 1398.304 kb on - strand at 1398.304 kb on - strand at 1398.326 kb on + strand, within MCAODC_23325 at 1398.566 kb on + strand, within MCAODC_23330
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,395,720 + cah MCAODC_23310 0.79 -1.1 1,395,722 + cah MCAODC_23310 0.79 -2.4 1,395,828 + cah MCAODC_23310 0.82 +2.5 1,396,160 - -0.7 1,396,392 + +0.2 1,396,393 - +0.1 1,396,595 - -2.7 1,396,674 - +1.6 1,397,020 - yeeP MCAODC_23315 0.37 -4.2 1,397,157 + yeeP MCAODC_23315 0.52 +1.3 1,397,157 + yeeP MCAODC_23315 0.52 +1.4 1,397,158 - yeeP MCAODC_23315 0.52 +0.3 1,397,158 - yeeP MCAODC_23315 0.52 -0.1 1,397,173 + yeeP MCAODC_23315 0.54 +0.4 1,397,220 + yeeP MCAODC_23315 0.59 +2.5 1,397,220 + yeeP MCAODC_23315 0.59 +1.6 1,397,263 + yeeP MCAODC_23315 0.64 +2.0 1,397,450 + yeeP MCAODC_23315 0.86 -0.0 1,397,573 + +1.2 1,397,573 + -0.9 1,397,573 + +1.0 1,397,573 + -0.9 1,397,573 + -0.4 1,397,573 + -0.5 1,397,573 + -0.2 1,397,573 + -1.1 1,397,574 - -2.0 1,397,574 - +0.2 1,397,579 + +1.0 1,397,579 + -2.6 1,397,580 - +0.7 1,397,580 - -1.6 1,397,674 - -3.1 1,397,690 - -0.6 1,397,690 - -4.1 1,397,690 - +1.6 1,397,713 - -2.4 1,397,906 + -3.2 1,397,939 - MCAODC_23320 0.21 +0.0 1,398,303 + -0.0 1,398,303 + -0.2 1,398,304 - -2.2 1,398,304 - -2.6 1,398,326 + MCAODC_23325 0.32 -1.7 1,398,566 + MCAODC_23330 0.43 -0.1
Or see this region's nucleotide sequence