Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiK and lpoB overlap by 20 nucleotides lpoB and MCAODC_22880 are separated by 13 nucleotides MCAODC_22880 and hinT are separated by 2 nucleotides hinT and fhuE are separated by 345 nucleotides
MCAODC_22870: thiK - thiamine kinase, at 1,317,288 to 1,318,112
thiK
MCAODC_22875: lpoB - penicillin-binding protein activator LpoB, at 1,318,093 to 1,318,734
lpoB
MCAODC_22880: MCAODC_22880 - DUF1425 domain-containing protein, at 1,318,748 to 1,319,125
_22880
MCAODC_22885: hinT - purine nucleoside phosphoramidase, at 1,319,128 to 1,319,487
hinT
MCAODC_22890: fhuE - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 1,319,833 to 1,322,022
fhuE
Position (kb) 1318
1319
1320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1317.778 kb on + strand, within thiK at 1317.782 kb on - strand, within thiK at 1317.868 kb on + strand, within thiK at 1317.869 kb on - strand, within thiK at 1317.870 kb on + strand, within thiK at 1317.870 kb on + strand, within thiK at 1317.870 kb on + strand, within thiK at 1317.870 kb on + strand, within thiK at 1317.871 kb on - strand, within thiK at 1317.871 kb on - strand, within thiK at 1318.025 kb on + strand, within thiK at 1318.026 kb on - strand, within thiK at 1318.029 kb on - strand, within thiK at 1318.095 kb on - strand at 1318.095 kb on - strand at 1318.116 kb on + strand at 1318.117 kb on - strand at 1318.203 kb on - strand, within lpoB at 1318.211 kb on + strand, within lpoB at 1318.252 kb on - strand, within lpoB at 1318.285 kb on + strand, within lpoB at 1318.286 kb on - strand, within lpoB at 1318.286 kb on - strand, within lpoB at 1318.286 kb on - strand, within lpoB at 1318.467 kb on + strand, within lpoB at 1318.467 kb on + strand, within lpoB at 1318.468 kb on - strand, within lpoB at 1318.932 kb on + strand, within MCAODC_22880 at 1318.932 kb on + strand, within MCAODC_22880 at 1318.933 kb on - strand, within MCAODC_22880 at 1319.084 kb on + strand, within MCAODC_22880 at 1319.085 kb on - strand, within MCAODC_22880 at 1319.085 kb on - strand, within MCAODC_22880 at 1319.129 kb on + strand at 1319.196 kb on - strand, within hinT at 1319.508 kb on - strand at 1319.528 kb on + strand at 1319.552 kb on - strand at 1319.552 kb on - strand at 1319.557 kb on - strand at 1319.627 kb on + strand at 1319.627 kb on + strand at 1319.629 kb on + strand at 1319.629 kb on + strand at 1319.629 kb on + strand at 1319.630 kb on - strand at 1319.630 kb on - strand at 1319.631 kb on + strand at 1319.632 kb on - strand at 1319.632 kb on - strand at 1319.632 kb on - strand at 1319.632 kb on - strand at 1319.632 kb on - strand at 1319.632 kb on - strand at 1319.641 kb on - strand at 1319.691 kb on + strand at 1319.770 kb on + strand at 1319.772 kb on + strand at 1319.906 kb on + strand at 1319.906 kb on + strand at 1320.082 kb on - strand, within fhuE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,317,778 + thiK MCAODC_22870 0.59 +0.1 1,317,782 - thiK MCAODC_22870 0.60 +1.1 1,317,868 + thiK MCAODC_22870 0.70 -0.1 1,317,869 - thiK MCAODC_22870 0.70 +1.7 1,317,870 + thiK MCAODC_22870 0.71 -2.3 1,317,870 + thiK MCAODC_22870 0.71 +1.2 1,317,870 + thiK MCAODC_22870 0.71 -0.6 1,317,870 + thiK MCAODC_22870 0.71 +2.4 1,317,871 - thiK MCAODC_22870 0.71 +0.9 1,317,871 - thiK MCAODC_22870 0.71 +0.9 1,318,025 + thiK MCAODC_22870 0.89 -1.3 1,318,026 - thiK MCAODC_22870 0.89 -1.5 1,318,029 - thiK MCAODC_22870 0.90 -1.9 1,318,095 - -3.2 1,318,095 - +1.0 1,318,116 + -1.1 1,318,117 - -0.4 1,318,203 - lpoB MCAODC_22875 0.17 +0.0 1,318,211 + lpoB MCAODC_22875 0.18 -0.2 1,318,252 - lpoB MCAODC_22875 0.25 -1.1 1,318,285 + lpoB MCAODC_22875 0.30 -2.4 1,318,286 - lpoB MCAODC_22875 0.30 +1.5 1,318,286 - lpoB MCAODC_22875 0.30 +1.4 1,318,286 - lpoB MCAODC_22875 0.30 -3.3 1,318,467 + lpoB MCAODC_22875 0.58 -1.0 1,318,467 + lpoB MCAODC_22875 0.58 +0.6 1,318,468 - lpoB MCAODC_22875 0.58 +0.4 1,318,932 + MCAODC_22880 0.49 -0.9 1,318,932 + MCAODC_22880 0.49 -2.1 1,318,933 - MCAODC_22880 0.49 -2.5 1,319,084 + MCAODC_22880 0.89 +2.0 1,319,085 - MCAODC_22880 0.89 -0.1 1,319,085 - MCAODC_22880 0.89 +3.1 1,319,129 + +0.7 1,319,196 - hinT MCAODC_22885 0.19 +0.9 1,319,508 - +1.0 1,319,528 + -3.7 1,319,552 - -1.0 1,319,552 - -2.1 1,319,557 - +0.4 1,319,627 + -3.2 1,319,627 + -0.7 1,319,629 + +0.0 1,319,629 + -2.5 1,319,629 + -2.0 1,319,630 - -0.5 1,319,630 - +0.5 1,319,631 + +1.2 1,319,632 - -0.2 1,319,632 - -1.9 1,319,632 - +1.3 1,319,632 - +1.1 1,319,632 - -2.6 1,319,632 - -2.6 1,319,641 - -2.0 1,319,691 + +0.1 1,319,770 + +0.1 1,319,772 + +0.6 1,319,906 + -0.2 1,319,906 + +0.3 1,320,082 - fhuE MCAODC_22890 0.11 +1.4
Or see this region's nucleotide sequence