Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_21510 and MCAODC_21515 overlap by 17 nucleotides MCAODC_21515 and hipB are separated by 76 nucleotides hipB and MCAODC_21525 are separated by 263 nucleotides MCAODC_21525 and MCAODC_21530 are separated by 72 nucleotides MCAODC_21530 and MCAODC_21535 are separated by 22 nucleotides MCAODC_21535 and MCAODC_21540 overlap by 23 nucleotides
MCAODC_21510: MCAODC_21510 - Putative bacterial toxin ydaT, at 1,090,644 to 1,091,195
_21510
MCAODC_21515: MCAODC_21515 - transcriptional regulator, at 1,091,179 to 1,091,406
_21515
MCAODC_21520: hipB - transcriptional regulator, at 1,091,483 to 1,091,890
hipB
MCAODC_21525: MCAODC_21525 - hypothetical protein, at 1,092,154 to 1,092,453
_21525
MCAODC_21530: MCAODC_21530 - Uncharacterized protein YdfC, at 1,092,526 to 1,092,744
_21530
MCAODC_21535: MCAODC_21535 - hypothetical protein, at 1,092,767 to 1,093,174
_21535
MCAODC_21540: MCAODC_21540 - hypothetical protein, at 1,093,152 to 1,093,385
_21540
Position (kb) 1092
1093 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1091.252 kb on + strand, within MCAODC_21515 at 1091.388 kb on + strand at 1091.388 kb on + strand at 1091.940 kb on + strand at 1092.163 kb on + strand at 1092.163 kb on + strand at 1092.164 kb on - strand at 1092.166 kb on - strand at 1092.195 kb on - strand, within MCAODC_21525 at 1092.195 kb on - strand, within MCAODC_21525 at 1092.563 kb on - strand, within MCAODC_21530 at 1092.742 kb on + strand at 1092.746 kb on - strand at 1092.783 kb on + strand at 1093.132 kb on + strand, within MCAODC_21535 at 1093.335 kb on + strand, within MCAODC_21540
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,091,252 + MCAODC_21515 0.32 -0.4 1,091,388 + -1.5 1,091,388 + +0.1 1,091,940 + -3.3 1,092,163 + +1.8 1,092,163 + +0.3 1,092,164 - +2.1 1,092,166 - +3.7 1,092,195 - MCAODC_21525 0.14 +0.8 1,092,195 - MCAODC_21525 0.14 -2.2 1,092,563 - MCAODC_21530 0.17 -1.2 1,092,742 + -2.4 1,092,746 - +1.0 1,092,783 + +1.4 1,093,132 + MCAODC_21535 0.89 -2.3 1,093,335 + MCAODC_21540 0.78 +1.3
Or see this region's nucleotide sequence