Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehI and MCAODC_21185 are separated by 61 nucleotides MCAODC_21185 and zorO are separated by 109 nucleotides zorO and zorP are separated by 519 nucleotides
MCAODC_21180: yehI - Uncharacterized protein YehI, at 1,038,889 to 1,042,518
yehI
MCAODC_21185: MCAODC_21185 - Protein, at 1,042,580 to 1,042,897
_21185
MCAODC_21190: zorO - Small toxic protein ZorO, at 1,043,007 to 1,043,096
zorO
MCAODC_21200: zorP - Small toxic protein ZorP, at 1,043,616 to 1,043,705
zorP
Position (kb)
1042
1043 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1041.599 kb on + strand, within yehI at 1041.626 kb on + strand, within yehI at 1041.690 kb on + strand, within yehI at 1041.881 kb on + strand, within yehI at 1041.885 kb on + strand, within yehI at 1041.885 kb on + strand, within yehI at 1041.886 kb on - strand, within yehI at 1041.886 kb on - strand, within yehI at 1041.886 kb on - strand, within yehI at 1041.965 kb on + strand, within yehI at 1041.968 kb on + strand, within yehI at 1042.164 kb on + strand at 1042.328 kb on - strand at 1042.375 kb on - strand at 1042.429 kb on + strand at 1042.563 kb on + strand at 1042.564 kb on - strand at 1042.607 kb on - strand at 1042.611 kb on - strand at 1042.666 kb on + strand, within MCAODC_21185 at 1042.681 kb on - strand, within MCAODC_21185 at 1042.786 kb on + strand, within MCAODC_21185 at 1042.788 kb on + strand, within MCAODC_21185 at 1042.949 kb on + strand at 1042.986 kb on - strand at 1043.074 kb on - strand, within zorO at 1043.324 kb on - strand at 1043.536 kb on - strand at 1043.652 kb on + strand, within zorP at 1043.664 kb on + strand, within zorP at 1043.665 kb on - strand, within zorP at 1043.771 kb on + strand at 1043.771 kb on + strand at 1043.775 kb on + strand at 1043.775 kb on + strand at 1043.853 kb on - strand at 1043.853 kb on - strand at 1043.890 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,041,599 + yehI MCAODC_21180 0.75 -2.4 1,041,626 + yehI MCAODC_21180 0.75 +1.3 1,041,690 + yehI MCAODC_21180 0.77 +4.1 1,041,881 + yehI MCAODC_21180 0.82 +1.2 1,041,885 + yehI MCAODC_21180 0.83 -0.5 1,041,885 + yehI MCAODC_21180 0.83 -0.0 1,041,886 - yehI MCAODC_21180 0.83 -1.9 1,041,886 - yehI MCAODC_21180 0.83 -0.5 1,041,886 - yehI MCAODC_21180 0.83 -0.1 1,041,965 + yehI MCAODC_21180 0.85 -1.2 1,041,968 + yehI MCAODC_21180 0.85 +0.9 1,042,164 + -0.0 1,042,328 - -1.1 1,042,375 - -1.0 1,042,429 + -1.0 1,042,563 + +0.3 1,042,564 - -1.6 1,042,607 - +0.2 1,042,611 - +0.8 1,042,666 + MCAODC_21185 0.27 +1.2 1,042,681 - MCAODC_21185 0.32 -1.1 1,042,786 + MCAODC_21185 0.65 +0.8 1,042,788 + MCAODC_21185 0.65 +0.1 1,042,949 + +0.4 1,042,986 - +0.3 1,043,074 - zorO MCAODC_21190 0.74 +1.1 1,043,324 - -3.0 1,043,536 - -0.5 1,043,652 + zorP MCAODC_21200 0.40 +0.8 1,043,664 + zorP MCAODC_21200 0.53 +0.0 1,043,665 - zorP MCAODC_21200 0.54 -0.9 1,043,771 + -0.0 1,043,771 + +0.3 1,043,775 + -2.2 1,043,775 + +1.6 1,043,853 - +1.0 1,043,853 - -0.2 1,043,890 - -1.1
Or see this region's nucleotide sequence