Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gatB and gatB are separated by 3 nucleotides gatB and gatA are separated by 30 nucleotides gatA and gatZ are separated by 9 nucleotides gatZ and MCAODC_21040 overlap by 59 nucleotides
MCAODC_21020: gatB - PTS galactitol transporter subunit IIB, at 1,006,314 to 1,007,669
gatB
MCAODC_21025: gatB - PTS galactitol transporter subunit IIB, at 1,007,673 to 1,007,957
gatB
MCAODC_21030: gatA - PTS galactitol transporter subunit IIA, at 1,007,988 to 1,008,440
gatA
MCAODC_21035: gatZ - tagatose-bisphosphate aldolase subunit GatZ, at 1,008,450 to 1,009,286
gatZ
MCAODC_21040: MCAODC_21040 - tagatose-bisphosphate aldolase, at 1,009,228 to 1,009,707
_21040
Position (kb)
1007
1008
1009 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1007.048 kb on + strand, within gatB at 1007.052 kb on - strand, within gatB at 1007.082 kb on - strand, within gatB at 1007.113 kb on + strand, within gatB at 1007.113 kb on + strand, within gatB at 1007.114 kb on - strand, within gatB at 1007.114 kb on - strand, within gatB at 1007.123 kb on + strand, within gatB at 1007.124 kb on - strand, within gatB at 1007.196 kb on - strand, within gatB at 1007.236 kb on - strand, within gatB at 1007.314 kb on - strand, within gatB at 1007.314 kb on - strand, within gatB at 1007.338 kb on - strand, within gatB at 1007.601 kb on - strand at 1007.819 kb on - strand, within gatB at 1008.082 kb on - strand, within gatA at 1008.220 kb on + strand, within gatA at 1008.441 kb on - strand at 1008.519 kb on + strand at 1008.861 kb on + strand, within gatZ at 1009.016 kb on + strand, within gatZ at 1009.074 kb on + strand, within gatZ at 1009.074 kb on + strand, within gatZ at 1009.100 kb on + strand, within gatZ at 1009.122 kb on + strand, within gatZ at 1009.123 kb on - strand, within gatZ at 1009.216 kb on + strand at 1009.216 kb on + strand at 1009.217 kb on - strand at 1009.351 kb on - strand, within MCAODC_21040 at 1009.370 kb on + strand, within MCAODC_21040 at 1009.370 kb on + strand, within MCAODC_21040 at 1009.370 kb on + strand, within MCAODC_21040 at 1009.370 kb on + strand, within MCAODC_21040 at 1009.370 kb on + strand, within MCAODC_21040 at 1009.371 kb on - strand, within MCAODC_21040 at 1009.371 kb on - strand, within MCAODC_21040 at 1009.371 kb on - strand, within MCAODC_21040
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,007,048 + gatB MCAODC_21020 0.54 +0.9 1,007,052 - gatB MCAODC_21020 0.54 +2.2 1,007,082 - gatB MCAODC_21020 0.57 +0.4 1,007,113 + gatB MCAODC_21020 0.59 -0.0 1,007,113 + gatB MCAODC_21020 0.59 -1.2 1,007,114 - gatB MCAODC_21020 0.59 +0.2 1,007,114 - gatB MCAODC_21020 0.59 +0.8 1,007,123 + gatB MCAODC_21020 0.60 -1.5 1,007,124 - gatB MCAODC_21020 0.60 -0.3 1,007,196 - gatB MCAODC_21020 0.65 +2.2 1,007,236 - gatB MCAODC_21020 0.68 -0.3 1,007,314 - gatB MCAODC_21020 0.74 +1.6 1,007,314 - gatB MCAODC_21020 0.74 -3.4 1,007,338 - gatB MCAODC_21020 0.76 -1.2 1,007,601 - +0.3 1,007,819 - gatB MCAODC_21025 0.51 +0.7 1,008,082 - gatA MCAODC_21030 0.21 -0.0 1,008,220 + gatA MCAODC_21030 0.51 -2.1 1,008,441 - +1.5 1,008,519 + -1.9 1,008,861 + gatZ MCAODC_21035 0.49 -1.7 1,009,016 + gatZ MCAODC_21035 0.68 -1.8 1,009,074 + gatZ MCAODC_21035 0.75 +0.2 1,009,074 + gatZ MCAODC_21035 0.75 -1.6 1,009,100 + gatZ MCAODC_21035 0.78 -1.2 1,009,122 + gatZ MCAODC_21035 0.80 -0.5 1,009,123 - gatZ MCAODC_21035 0.80 +0.3 1,009,216 + +1.3 1,009,216 + +1.3 1,009,217 - -0.0 1,009,351 - MCAODC_21040 0.26 +0.1 1,009,370 + MCAODC_21040 0.30 +2.2 1,009,370 + MCAODC_21040 0.30 -2.2 1,009,370 + MCAODC_21040 0.30 -0.7 1,009,370 + MCAODC_21040 0.30 -1.4 1,009,370 + MCAODC_21040 0.30 +0.8 1,009,371 - MCAODC_21040 0.30 +1.1 1,009,371 - MCAODC_21040 0.30 -1.6 1,009,371 - MCAODC_21040 0.30 +0.1
Or see this region's nucleotide sequence