Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegI and pphC overlap by 4 nucleotides pphC and terY are separated by 77 nucleotides terY and ibsA are separated by 220 nucleotides ibsA and MCAODC_20725 are separated by 271 nucleotides MCAODC_20725 and MCAODC_20735 are separated by 271 nucleotides
MCAODC_20695: yegI - protein kinase YegI, at 952,769 to 954,709
yegI
MCAODC_20700: pphC - Serine/threonine-protein phosphatase 3, at 954,706 to 955,386
pphC
MCAODC_20705: terY - Uncharacterized protein YegL, at 955,464 to 956,123
terY
MCAODC_20715: ibsA - Small toxic protein IbsA, at 956,344 to 956,403
ibsA
MCAODC_20725: MCAODC_20725 - Type I toxin-antitoxin system Ibs family toxin, at 956,675 to 956,731
_20725
MCAODC_20735: MCAODC_20735 - Type I toxin-antitoxin system Ibs family toxin, at 957,003 to 957,059
_20735
Position (kb) 955
956
957 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 954.511 kb on - strand, within yegI at 954.544 kb on + strand at 954.544 kb on + strand at 954.628 kb on - strand at 954.691 kb on + strand at 954.691 kb on + strand at 954.691 kb on + strand at 954.692 kb on - strand at 954.692 kb on - strand at 954.692 kb on - strand at 954.693 kb on + strand at 954.693 kb on + strand at 954.693 kb on + strand at 954.694 kb on - strand at 954.694 kb on - strand at 954.695 kb on + strand at 954.696 kb on - strand at 954.696 kb on - strand at 954.712 kb on + strand at 954.738 kb on + strand at 954.739 kb on - strand at 955.010 kb on + strand, within pphC at 955.011 kb on - strand, within pphC at 955.030 kb on - strand, within pphC at 955.037 kb on - strand, within pphC at 955.037 kb on - strand, within pphC at 955.153 kb on + strand, within pphC at 955.206 kb on + strand, within pphC at 955.265 kb on + strand, within pphC at 955.266 kb on - strand, within pphC at 955.266 kb on - strand, within pphC at 955.266 kb on - strand, within pphC at 955.408 kb on + strand at 955.408 kb on + strand at 955.409 kb on - strand at 955.409 kb on - strand at 955.409 kb on - strand at 955.449 kb on - strand at 955.576 kb on - strand, within terY at 955.625 kb on + strand, within terY at 955.671 kb on - strand, within terY at 955.671 kb on - strand, within terY at 955.760 kb on - strand, within terY at 955.763 kb on - strand, within terY at 955.770 kb on - strand, within terY at 955.932 kb on - strand, within terY at 955.932 kb on - strand, within terY at 955.989 kb on - strand, within terY at 956.138 kb on + strand at 956.164 kb on + strand at 956.185 kb on - strand at 956.240 kb on - strand at 956.277 kb on + strand at 956.278 kb on - strand at 956.345 kb on + strand at 956.345 kb on + strand at 956.345 kb on + strand at 956.346 kb on - strand at 956.349 kb on - strand at 956.349 kb on - strand at 956.361 kb on + strand, within ibsA at 956.362 kb on - strand, within ibsA at 956.369 kb on - strand, within ibsA at 956.374 kb on + strand, within ibsA at 956.374 kb on + strand, within ibsA at 956.383 kb on + strand, within ibsA at 956.384 kb on - strand, within ibsA at 956.404 kb on - strand at 956.567 kb on - strand at 956.670 kb on + strand at 956.679 kb on + strand at 956.693 kb on + strand, within MCAODC_20725 at 956.693 kb on + strand, within MCAODC_20725 at 956.693 kb on + strand, within MCAODC_20725 at 956.694 kb on - strand, within MCAODC_20725 at 956.699 kb on + strand, within MCAODC_20725 at 956.748 kb on - strand at 956.748 kb on - strand at 956.998 kb on + strand at 956.998 kb on + strand at 957.004 kb on + strand at 957.004 kb on + strand at 957.004 kb on + strand at 957.007 kb on + strand at 957.007 kb on + strand at 957.017 kb on - strand, within MCAODC_20735 at 957.020 kb on + strand, within MCAODC_20735 at 957.048 kb on + strand, within MCAODC_20735 at 957.049 kb on - strand, within MCAODC_20735 at 957.071 kb on + strand at 957.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 954,511 - yegI MCAODC_20695 0.90 -1.0 954,544 + -3.5 954,544 + +0.2 954,628 - -3.2 954,691 + +1.7 954,691 + +0.6 954,691 + -1.5 954,692 - -3.5 954,692 - -0.1 954,692 - +2.5 954,693 + -0.3 954,693 + +0.1 954,693 + +1.0 954,694 - -0.1 954,694 - -0.9 954,695 + +0.1 954,696 - +2.1 954,696 - +0.9 954,712 + -3.6 954,738 + -0.2 954,739 - +2.4 955,010 + pphC MCAODC_20700 0.45 +0.7 955,011 - pphC MCAODC_20700 0.45 -0.2 955,030 - pphC MCAODC_20700 0.48 -1.7 955,037 - pphC MCAODC_20700 0.49 -1.5 955,037 - pphC MCAODC_20700 0.49 -3.3 955,153 + pphC MCAODC_20700 0.66 +1.4 955,206 + pphC MCAODC_20700 0.73 +1.4 955,265 + pphC MCAODC_20700 0.82 -3.5 955,266 - pphC MCAODC_20700 0.82 -1.8 955,266 - pphC MCAODC_20700 0.82 -1.8 955,266 - pphC MCAODC_20700 0.82 -0.4 955,408 + -0.1 955,408 + +1.2 955,409 - -1.7 955,409 - -1.4 955,409 - +0.4 955,449 - -4.4 955,576 - terY MCAODC_20705 0.17 +1.1 955,625 + terY MCAODC_20705 0.24 -2.6 955,671 - terY MCAODC_20705 0.31 -1.2 955,671 - terY MCAODC_20705 0.31 +1.0 955,760 - terY MCAODC_20705 0.45 +1.0 955,763 - terY MCAODC_20705 0.45 +1.1 955,770 - terY MCAODC_20705 0.46 -3.3 955,932 - terY MCAODC_20705 0.71 +0.2 955,932 - terY MCAODC_20705 0.71 +1.1 955,989 - terY MCAODC_20705 0.80 -0.1 956,138 + +1.3 956,164 + -0.3 956,185 - -0.8 956,240 - -1.5 956,277 + -0.4 956,278 - -0.9 956,345 + +2.3 956,345 + +0.8 956,345 + -2.4 956,346 - -0.2 956,349 - +0.4 956,349 - -0.8 956,361 + ibsA MCAODC_20715 0.28 -2.0 956,362 - ibsA MCAODC_20715 0.30 -0.9 956,369 - ibsA MCAODC_20715 0.42 -0.8 956,374 + ibsA MCAODC_20715 0.50 +0.5 956,374 + ibsA MCAODC_20715 0.50 +1.9 956,383 + ibsA MCAODC_20715 0.65 +1.4 956,384 - ibsA MCAODC_20715 0.67 +0.9 956,404 - -3.4 956,567 - -4.5 956,670 + +0.7 956,679 + -3.5 956,693 + MCAODC_20725 0.32 +1.2 956,693 + MCAODC_20725 0.32 -0.9 956,693 + MCAODC_20725 0.32 -2.2 956,694 - MCAODC_20725 0.33 -0.9 956,699 + MCAODC_20725 0.42 +1.3 956,748 - -1.4 956,748 - +0.8 956,998 + -0.1 956,998 + -0.8 957,004 + +0.5 957,004 + -0.1 957,004 + -1.2 957,007 + +0.0 957,007 + +1.0 957,017 - MCAODC_20735 0.25 -0.1 957,020 + MCAODC_20735 0.30 -0.3 957,048 + MCAODC_20735 0.79 +0.9 957,049 - MCAODC_20735 0.81 +0.6 957,071 + -3.1 957,075 + -0.3
Or see this region's nucleotide sequence