Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20320 and MCAODC_20325 are separated by 81 nucleotides MCAODC_20325 and yeeS are separated by 14 nucleotides yeeS and yeeT are separated by 62 nucleotides yeeT and cbtA are separated by 73 nucleotides cbtA and MCAODC_20345 are separated by 88 nucleotides
MCAODC_20320: MCAODC_20320 - DUF932 domain-containing protein, at 875,278 to 876,099
_20320
MCAODC_20325: MCAODC_20325 - restriction endonuclease, at 876,181 to 876,660
_20325
MCAODC_20330: yeeS - UPF0758 protein YeeS, at 876,675 to 877,151
yeeS
MCAODC_20335: yeeT - Uncharacterized protein YeeT, at 877,214 to 877,435
yeeT
MCAODC_20340: cbtA - type IV toxin-antitoxin system toxin CbtA, at 877,509 to 877,877
cbtA
MCAODC_20345: MCAODC_20345 - toxin of the YeeV-YeeU toxin-antitoxin system, at 877,966 to 878,229
_20345
Position (kb) 876
877
878 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 875.959 kb on - strand, within MCAODC_20320 at 876.222 kb on + strand at 876.468 kb on - strand, within MCAODC_20325 at 876.544 kb on + strand, within MCAODC_20325 at 876.544 kb on + strand, within MCAODC_20325 at 876.574 kb on + strand, within MCAODC_20325 at 876.575 kb on - strand, within MCAODC_20325 at 876.592 kb on + strand, within MCAODC_20325 at 877.186 kb on + strand at 877.404 kb on - strand, within yeeT at 877.404 kb on - strand, within yeeT at 877.469 kb on - strand at 877.469 kb on - strand at 877.937 kb on + strand at 877.937 kb on + strand at 877.939 kb on + strand at 877.982 kb on + strand at 877.983 kb on - strand at 877.983 kb on - strand at 877.985 kb on + strand at 878.036 kb on + strand, within MCAODC_20345 at 878.036 kb on + strand, within MCAODC_20345 at 878.037 kb on - strand, within MCAODC_20345 at 878.065 kb on + strand, within MCAODC_20345
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 875,959 - MCAODC_20320 0.83 -1.5 876,222 + +1.0 876,468 - MCAODC_20325 0.60 -1.5 876,544 + MCAODC_20325 0.76 -1.5 876,544 + MCAODC_20325 0.76 -4.1 876,574 + MCAODC_20325 0.82 -0.3 876,575 - MCAODC_20325 0.82 +0.7 876,592 + MCAODC_20325 0.86 +0.1 877,186 + -1.6 877,404 - yeeT MCAODC_20335 0.86 +1.7 877,404 - yeeT MCAODC_20335 0.86 +1.7 877,469 - +3.5 877,469 - +0.3 877,937 + -1.3 877,937 + -0.2 877,939 + +1.9 877,982 + -0.1 877,983 - +0.1 877,983 - +0.8 877,985 + -1.9 878,036 + MCAODC_20345 0.27 -0.7 878,036 + MCAODC_20345 0.27 +1.2 878,037 - MCAODC_20345 0.27 -1.2 878,065 + MCAODC_20345 0.38 -1.3
Or see this region's nucleotide sequence