Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobT and cobS are separated by 11 nucleotides cobS and cobU overlap by 4 nucleotides cobU and insE are separated by 364 nucleotides insE and tnpB overlap by 4 nucleotides
MCAODC_20255: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 862,062 to 863,141
cobT
MCAODC_20260: cobS - adenosylcobinamide-GDP ribazoletransferase, at 863,153 to 863,896
cobS
MCAODC_20265: cobU - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 863,893 to 864,435
cobU
MCAODC_20270: insE - Transposase InsE and inactivated derivatives, at 864,800 to 865,180
insE
MCAODC_20275: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 865,177 to 865,524
tnpB
Position (kb) 863
864
865 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 862.946 kb on + strand, within cobT at 863.011 kb on - strand, within cobT at 863.011 kb on - strand, within cobT at 863.115 kb on - strand at 863.285 kb on - strand, within cobS at 863.315 kb on + strand, within cobS at 863.315 kb on + strand, within cobS at 863.316 kb on - strand, within cobS at 863.337 kb on - strand, within cobS at 863.412 kb on + strand, within cobS at 863.424 kb on + strand, within cobS at 863.424 kb on + strand, within cobS at 863.424 kb on + strand, within cobS at 863.425 kb on - strand, within cobS at 863.425 kb on - strand, within cobS at 863.535 kb on - strand, within cobS at 863.571 kb on - strand, within cobS at 863.579 kb on - strand, within cobS at 863.612 kb on - strand, within cobS at 863.624 kb on + strand, within cobS at 863.638 kb on + strand, within cobS at 863.792 kb on - strand, within cobS at 863.795 kb on - strand, within cobS at 863.809 kb on - strand, within cobS at 863.933 kb on - strand at 863.933 kb on - strand at 863.980 kb on - strand, within cobU at 864.043 kb on + strand, within cobU at 864.044 kb on - strand, within cobU at 864.047 kb on - strand, within cobU at 864.047 kb on - strand, within cobU at 864.047 kb on - strand, within cobU at 864.103 kb on - strand, within cobU at 864.308 kb on - strand, within cobU at 864.345 kb on + strand, within cobU at 864.351 kb on + strand, within cobU at 864.353 kb on + strand, within cobU at 864.354 kb on - strand, within cobU at 864.354 kb on - strand, within cobU at 864.354 kb on - strand, within cobU at 864.459 kb on + strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.460 kb on - strand at 864.468 kb on - strand at 864.531 kb on - strand at 864.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 862,946 + cobT MCAODC_20255 0.82 -0.4 863,011 - cobT MCAODC_20255 0.88 +1.5 863,011 - cobT MCAODC_20255 0.88 -0.8 863,115 - -0.2 863,285 - cobS MCAODC_20260 0.18 -2.6 863,315 + cobS MCAODC_20260 0.22 -1.3 863,315 + cobS MCAODC_20260 0.22 -0.3 863,316 - cobS MCAODC_20260 0.22 +0.0 863,337 - cobS MCAODC_20260 0.25 +0.8 863,412 + cobS MCAODC_20260 0.35 +1.0 863,424 + cobS MCAODC_20260 0.36 +1.8 863,424 + cobS MCAODC_20260 0.36 -2.1 863,424 + cobS MCAODC_20260 0.36 -4.0 863,425 - cobS MCAODC_20260 0.37 +0.0 863,425 - cobS MCAODC_20260 0.37 +1.2 863,535 - cobS MCAODC_20260 0.51 +3.2 863,571 - cobS MCAODC_20260 0.56 +1.1 863,579 - cobS MCAODC_20260 0.57 +0.6 863,612 - cobS MCAODC_20260 0.62 -0.1 863,624 + cobS MCAODC_20260 0.63 +2.7 863,638 + cobS MCAODC_20260 0.65 +2.1 863,792 - cobS MCAODC_20260 0.86 -2.3 863,795 - cobS MCAODC_20260 0.86 +0.3 863,809 - cobS MCAODC_20260 0.88 +0.0 863,933 - -1.2 863,933 - -0.0 863,980 - cobU MCAODC_20265 0.16 -1.9 864,043 + cobU MCAODC_20265 0.28 +1.0 864,044 - cobU MCAODC_20265 0.28 -2.1 864,047 - cobU MCAODC_20265 0.28 +1.0 864,047 - cobU MCAODC_20265 0.28 -0.7 864,047 - cobU MCAODC_20265 0.28 +0.5 864,103 - cobU MCAODC_20265 0.39 -2.2 864,308 - cobU MCAODC_20265 0.76 +2.2 864,345 + cobU MCAODC_20265 0.83 +1.6 864,351 + cobU MCAODC_20265 0.84 +0.9 864,353 + cobU MCAODC_20265 0.85 -0.3 864,354 - cobU MCAODC_20265 0.85 +0.7 864,354 - cobU MCAODC_20265 0.85 +1.9 864,354 - cobU MCAODC_20265 0.85 +2.5 864,459 + -3.5 864,460 - -1.2 864,460 - -1.0 864,460 - +0.5 864,460 - -0.2 864,460 - -4.6 864,460 - -1.2 864,460 - -0.6 864,460 - -0.3 864,460 - -0.1 864,468 - +0.7 864,531 - +1.8 864,710 - -3.1
Or see this region's nucleotide sequence