Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cbl and nac are separated by 101 nucleotides nac and Asn_trna are separated by 325 nucleotides Asn_trna and ldtA are separated by 55 nucleotides
MCAODC_20235: cbl - HTH-type transcriptional regulator Cbl, at 858,639 to 859,589
cbl
MCAODC_20240: nac - nitrogen assimilation transcriptional regulator NAC, at 859,691 to 860,608
nac
MCAODC_20245: Asn_trna - tRNA-Asn(gtt), at 860,934 to 861,009
_trna
MCAODC_20250: ldtA - L,D-transpeptidase, at 861,065 to 862,000
ldtA
Position (kb) 859
860
861 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 858.743 kb on - strand, within cbl at 858.747 kb on - strand, within cbl at 858.785 kb on + strand, within cbl at 858.785 kb on + strand, within cbl at 858.786 kb on - strand, within cbl at 858.788 kb on + strand, within cbl at 858.789 kb on - strand, within cbl at 858.789 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.892 kb on + strand, within cbl at 858.893 kb on - strand, within cbl at 858.893 kb on - strand, within cbl at 858.900 kb on - strand, within cbl at 858.924 kb on + strand, within cbl at 858.924 kb on + strand, within cbl at 858.927 kb on + strand, within cbl at 858.928 kb on - strand, within cbl at 858.928 kb on - strand, within cbl at 858.929 kb on + strand, within cbl at 858.929 kb on + strand, within cbl at 858.932 kb on + strand, within cbl at 858.933 kb on - strand, within cbl at 858.971 kb on + strand, within cbl at 858.972 kb on - strand, within cbl at 858.986 kb on + strand, within cbl at 858.986 kb on + strand, within cbl at 859.047 kb on - strand, within cbl at 859.085 kb on + strand, within cbl at 859.086 kb on - strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.272 kb on - strand, within cbl at 859.272 kb on - strand, within cbl at 859.274 kb on - strand, within cbl at 859.286 kb on + strand, within cbl at 859.287 kb on - strand, within cbl at 859.319 kb on + strand, within cbl at 859.373 kb on + strand, within cbl at 859.374 kb on - strand, within cbl at 859.392 kb on + strand, within cbl at 859.518 kb on - strand at 859.541 kb on - strand at 859.567 kb on - strand at 859.572 kb on + strand at 859.627 kb on - strand at 859.685 kb on + strand at 859.735 kb on + strand at 859.749 kb on - strand at 859.749 kb on - strand at 859.782 kb on - strand at 859.899 kb on - strand, within nac at 859.899 kb on - strand, within nac at 859.940 kb on - strand, within nac at 859.946 kb on - strand, within nac at 860.166 kb on + strand, within nac at 860.238 kb on + strand, within nac at 860.239 kb on - strand, within nac at 860.239 kb on - strand, within nac at 860.243 kb on - strand, within nac at 860.276 kb on + strand, within nac at 860.393 kb on + strand, within nac at 860.566 kb on + strand at 860.653 kb on + strand at 860.654 kb on - strand at 860.654 kb on - strand at 860.736 kb on - strand at 860.911 kb on - strand at 861.050 kb on + strand at 861.050 kb on + strand at 861.055 kb on - strand at 861.091 kb on + strand at 861.091 kb on + strand at 861.166 kb on + strand, within ldtA at 861.288 kb on + strand, within ldtA at 861.289 kb on - strand, within ldtA at 861.326 kb on + strand, within ldtA at 861.435 kb on + strand, within ldtA at 861.437 kb on + strand, within ldtA at 861.438 kb on - strand, within ldtA at 861.438 kb on - strand, within ldtA at 861.438 kb on - strand, within ldtA at 861.451 kb on - strand, within ldtA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 858,743 - cbl MCAODC_20235 0.11 -0.2 858,747 - cbl MCAODC_20235 0.11 -0.7 858,785 + cbl MCAODC_20235 0.15 +2.6 858,785 + cbl MCAODC_20235 0.15 +0.3 858,786 - cbl MCAODC_20235 0.15 +0.7 858,788 + cbl MCAODC_20235 0.16 -1.1 858,789 - cbl MCAODC_20235 0.16 +1.2 858,789 - cbl MCAODC_20235 0.16 +0.1 858,865 - cbl MCAODC_20235 0.24 -0.9 858,865 - cbl MCAODC_20235 0.24 -2.1 858,892 + cbl MCAODC_20235 0.27 +2.4 858,893 - cbl MCAODC_20235 0.27 -3.1 858,893 - cbl MCAODC_20235 0.27 -3.6 858,900 - cbl MCAODC_20235 0.27 +1.4 858,924 + cbl MCAODC_20235 0.30 +1.2 858,924 + cbl MCAODC_20235 0.30 -2.1 858,927 + cbl MCAODC_20235 0.30 +0.3 858,928 - cbl MCAODC_20235 0.30 -2.0 858,928 - cbl MCAODC_20235 0.30 +1.4 858,929 + cbl MCAODC_20235 0.30 -2.9 858,929 + cbl MCAODC_20235 0.30 +0.5 858,932 + cbl MCAODC_20235 0.31 +0.7 858,933 - cbl MCAODC_20235 0.31 -3.5 858,971 + cbl MCAODC_20235 0.35 -2.5 858,972 - cbl MCAODC_20235 0.35 +1.2 858,986 + cbl MCAODC_20235 0.36 -3.4 858,986 + cbl MCAODC_20235 0.36 -0.1 859,047 - cbl MCAODC_20235 0.43 -0.9 859,085 + cbl MCAODC_20235 0.47 +2.7 859,086 - cbl MCAODC_20235 0.47 +1.7 859,271 + cbl MCAODC_20235 0.66 -2.1 859,271 + cbl MCAODC_20235 0.66 +0.9 859,271 + cbl MCAODC_20235 0.66 -1.9 859,271 + cbl MCAODC_20235 0.66 -0.4 859,272 - cbl MCAODC_20235 0.67 +0.7 859,272 - cbl MCAODC_20235 0.67 +1.9 859,274 - cbl MCAODC_20235 0.67 +0.3 859,286 + cbl MCAODC_20235 0.68 +1.2 859,287 - cbl MCAODC_20235 0.68 -1.9 859,319 + cbl MCAODC_20235 0.72 +0.8 859,373 + cbl MCAODC_20235 0.77 +1.7 859,374 - cbl MCAODC_20235 0.77 -0.8 859,392 + cbl MCAODC_20235 0.79 -3.2 859,518 - -0.3 859,541 - -0.5 859,567 - +0.6 859,572 + -0.0 859,627 - -0.9 859,685 + +0.5 859,735 + +1.1 859,749 - -0.1 859,749 - -1.1 859,782 - -0.2 859,899 - nac MCAODC_20240 0.23 -0.1 859,899 - nac MCAODC_20240 0.23 +1.9 859,940 - nac MCAODC_20240 0.27 -0.9 859,946 - nac MCAODC_20240 0.28 -2.3 860,166 + nac MCAODC_20240 0.52 -0.5 860,238 + nac MCAODC_20240 0.60 +2.9 860,239 - nac MCAODC_20240 0.60 +0.5 860,239 - nac MCAODC_20240 0.60 +0.9 860,243 - nac MCAODC_20240 0.60 +0.3 860,276 + nac MCAODC_20240 0.64 -3.4 860,393 + nac MCAODC_20240 0.76 -0.3 860,566 + -2.7 860,653 + +1.0 860,654 - -1.9 860,654 - +1.0 860,736 - +0.2 860,911 - -0.3 861,050 + +1.0 861,050 + +0.5 861,055 - -2.9 861,091 + -0.6 861,091 + -0.5 861,166 + ldtA MCAODC_20250 0.11 +0.5 861,288 + ldtA MCAODC_20250 0.24 -0.2 861,289 - ldtA MCAODC_20250 0.24 +2.8 861,326 + ldtA MCAODC_20250 0.28 -1.1 861,435 + ldtA MCAODC_20250 0.40 +0.1 861,437 + ldtA MCAODC_20250 0.40 -0.1 861,438 - ldtA MCAODC_20250 0.40 -3.0 861,438 - ldtA MCAODC_20250 0.40 +0.5 861,438 - ldtA MCAODC_20250 0.40 +0.4 861,451 - ldtA MCAODC_20250 0.41 +0.2
Or see this region's nucleotide sequence