Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeO and Asn_trna are separated by 193 nucleotides Asn_trna and cbl are separated by 37 nucleotides cbl and nac are separated by 101 nucleotides
MCAODC_20225: yeeO - toxic metabolite efflux MATE transporter YeeO, at 856,878 to 858,332
yeeO
MCAODC_20230: Asn_trna - tRNA-Asn(gtt), at 858,526 to 858,601
_trna
MCAODC_20235: cbl - HTH-type transcriptional regulator Cbl, at 858,639 to 859,589
cbl
MCAODC_20240: nac - nitrogen assimilation transcriptional regulator NAC, at 859,691 to 860,608
nac
Position (kb) 858
859
860 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 857.747 kb on + strand, within yeeO at 857.748 kb on - strand, within yeeO at 857.753 kb on + strand, within yeeO at 857.753 kb on + strand, within yeeO at 857.898 kb on - strand, within yeeO at 857.957 kb on + strand, within yeeO at 858.069 kb on - strand, within yeeO at 858.072 kb on - strand, within yeeO at 858.241 kb on - strand at 858.265 kb on + strand at 858.265 kb on + strand at 858.342 kb on + strand at 858.444 kb on + strand at 858.444 kb on + strand at 858.743 kb on - strand, within cbl at 858.747 kb on - strand, within cbl at 858.785 kb on + strand, within cbl at 858.785 kb on + strand, within cbl at 858.786 kb on - strand, within cbl at 858.788 kb on + strand, within cbl at 858.789 kb on - strand, within cbl at 858.789 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.892 kb on + strand, within cbl at 858.893 kb on - strand, within cbl at 858.893 kb on - strand, within cbl at 858.900 kb on - strand, within cbl at 858.924 kb on + strand, within cbl at 858.924 kb on + strand, within cbl at 858.927 kb on + strand, within cbl at 858.928 kb on - strand, within cbl at 858.928 kb on - strand, within cbl at 858.929 kb on + strand, within cbl at 858.929 kb on + strand, within cbl at 858.932 kb on + strand, within cbl at 858.933 kb on - strand, within cbl at 858.971 kb on + strand, within cbl at 858.972 kb on - strand, within cbl at 858.986 kb on + strand, within cbl at 858.986 kb on + strand, within cbl at 859.047 kb on - strand, within cbl at 859.085 kb on + strand, within cbl at 859.086 kb on - strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.272 kb on - strand, within cbl at 859.272 kb on - strand, within cbl at 859.274 kb on - strand, within cbl at 859.286 kb on + strand, within cbl at 859.287 kb on - strand, within cbl at 859.319 kb on + strand, within cbl at 859.373 kb on + strand, within cbl at 859.374 kb on - strand, within cbl at 859.392 kb on + strand, within cbl at 859.518 kb on - strand at 859.541 kb on - strand at 859.567 kb on - strand at 859.572 kb on + strand at 859.627 kb on - strand at 859.685 kb on + strand at 859.735 kb on + strand at 859.749 kb on - strand at 859.749 kb on - strand at 859.782 kb on - strand at 859.899 kb on - strand, within nac at 859.899 kb on - strand, within nac at 859.940 kb on - strand, within nac at 859.946 kb on - strand, within nac at 860.166 kb on + strand, within nac at 860.238 kb on + strand, within nac at 860.239 kb on - strand, within nac at 860.239 kb on - strand, within nac at 860.243 kb on - strand, within nac at 860.276 kb on + strand, within nac at 860.393 kb on + strand, within nac at 860.566 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 857,747 + yeeO MCAODC_20225 0.60 +0.8 857,748 - yeeO MCAODC_20225 0.60 -0.5 857,753 + yeeO MCAODC_20225 0.60 -0.8 857,753 + yeeO MCAODC_20225 0.60 +0.8 857,898 - yeeO MCAODC_20225 0.70 +0.5 857,957 + yeeO MCAODC_20225 0.74 -2.9 858,069 - yeeO MCAODC_20225 0.82 +1.0 858,072 - yeeO MCAODC_20225 0.82 -1.4 858,241 - +0.3 858,265 + +1.3 858,265 + -2.2 858,342 + +0.1 858,444 + -0.7 858,444 + -2.2 858,743 - cbl MCAODC_20235 0.11 -0.2 858,747 - cbl MCAODC_20235 0.11 -0.7 858,785 + cbl MCAODC_20235 0.15 +2.6 858,785 + cbl MCAODC_20235 0.15 +0.3 858,786 - cbl MCAODC_20235 0.15 +0.7 858,788 + cbl MCAODC_20235 0.16 -1.1 858,789 - cbl MCAODC_20235 0.16 +1.2 858,789 - cbl MCAODC_20235 0.16 +0.1 858,865 - cbl MCAODC_20235 0.24 -0.9 858,865 - cbl MCAODC_20235 0.24 -2.1 858,892 + cbl MCAODC_20235 0.27 +2.4 858,893 - cbl MCAODC_20235 0.27 -3.1 858,893 - cbl MCAODC_20235 0.27 -3.6 858,900 - cbl MCAODC_20235 0.27 +1.4 858,924 + cbl MCAODC_20235 0.30 +1.2 858,924 + cbl MCAODC_20235 0.30 -2.1 858,927 + cbl MCAODC_20235 0.30 +0.3 858,928 - cbl MCAODC_20235 0.30 -2.0 858,928 - cbl MCAODC_20235 0.30 +1.4 858,929 + cbl MCAODC_20235 0.30 -2.9 858,929 + cbl MCAODC_20235 0.30 +0.5 858,932 + cbl MCAODC_20235 0.31 +0.7 858,933 - cbl MCAODC_20235 0.31 -3.5 858,971 + cbl MCAODC_20235 0.35 -2.5 858,972 - cbl MCAODC_20235 0.35 +1.2 858,986 + cbl MCAODC_20235 0.36 -3.4 858,986 + cbl MCAODC_20235 0.36 -0.1 859,047 - cbl MCAODC_20235 0.43 -0.9 859,085 + cbl MCAODC_20235 0.47 +2.7 859,086 - cbl MCAODC_20235 0.47 +1.7 859,271 + cbl MCAODC_20235 0.66 -2.1 859,271 + cbl MCAODC_20235 0.66 +0.9 859,271 + cbl MCAODC_20235 0.66 -1.9 859,271 + cbl MCAODC_20235 0.66 -0.4 859,272 - cbl MCAODC_20235 0.67 +0.7 859,272 - cbl MCAODC_20235 0.67 +1.9 859,274 - cbl MCAODC_20235 0.67 +0.3 859,286 + cbl MCAODC_20235 0.68 +1.2 859,287 - cbl MCAODC_20235 0.68 -1.9 859,319 + cbl MCAODC_20235 0.72 +0.8 859,373 + cbl MCAODC_20235 0.77 +1.7 859,374 - cbl MCAODC_20235 0.77 -0.8 859,392 + cbl MCAODC_20235 0.79 -3.2 859,518 - -0.3 859,541 - -0.5 859,567 - +0.6 859,572 + -0.0 859,627 - -0.9 859,685 + +0.5 859,735 + +1.1 859,749 - -0.1 859,749 - -1.1 859,782 - -0.2 859,899 - nac MCAODC_20240 0.23 -0.1 859,899 - nac MCAODC_20240 0.23 +1.9 859,940 - nac MCAODC_20240 0.27 -0.9 859,946 - nac MCAODC_20240 0.28 -2.3 860,166 + nac MCAODC_20240 0.52 -0.5 860,238 + nac MCAODC_20240 0.60 +2.9 860,239 - nac MCAODC_20240 0.60 +0.5 860,239 - nac MCAODC_20240 0.60 +0.9 860,243 - nac MCAODC_20240 0.60 +0.3 860,276 + nac MCAODC_20240 0.64 -3.4 860,393 + nac MCAODC_20240 0.76 -0.3 860,566 + -2.7
Or see this region's nucleotide sequence