Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdB and Asn_trna are separated by 449 nucleotides Asn_trna and yeeO are separated by 100 nucleotides yeeO and Asn_trna are separated by 193 nucleotides Asn_trna and cbl are separated by 37 nucleotides
MCAODC_20215: ycdB - putative transcriptional regulatory protein YcdB, at 855,536 to 856,252
ycdB
MCAODC_20220: Asn_trna - tRNA-Asn(gtt), at 856,702 to 856,777
_trna
MCAODC_20225: yeeO - toxic metabolite efflux MATE transporter YeeO, at 856,878 to 858,332
yeeO
MCAODC_20230: Asn_trna - tRNA-Asn(gtt), at 858,526 to 858,601
_trna
MCAODC_20235: cbl - HTH-type transcriptional regulator Cbl, at 858,639 to 859,589
cbl
Position (kb) 856
857
858
859 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 856.042 kb on + strand, within ycdB at 856.046 kb on + strand, within ycdB at 856.345 kb on - strand at 856.679 kb on - strand at 856.769 kb on + strand, within Asn_trna at 857.102 kb on + strand, within yeeO at 857.130 kb on - strand, within yeeO at 857.130 kb on - strand, within yeeO at 857.143 kb on - strand, within yeeO at 857.172 kb on - strand, within yeeO at 857.213 kb on + strand, within yeeO at 857.378 kb on + strand, within yeeO at 857.378 kb on + strand, within yeeO at 857.414 kb on + strand, within yeeO at 857.415 kb on - strand, within yeeO at 857.494 kb on - strand, within yeeO at 857.747 kb on + strand, within yeeO at 857.748 kb on - strand, within yeeO at 857.753 kb on + strand, within yeeO at 857.753 kb on + strand, within yeeO at 857.898 kb on - strand, within yeeO at 857.957 kb on + strand, within yeeO at 858.069 kb on - strand, within yeeO at 858.072 kb on - strand, within yeeO at 858.241 kb on - strand at 858.265 kb on + strand at 858.265 kb on + strand at 858.342 kb on + strand at 858.444 kb on + strand at 858.444 kb on + strand at 858.743 kb on - strand, within cbl at 858.747 kb on - strand, within cbl at 858.785 kb on + strand, within cbl at 858.785 kb on + strand, within cbl at 858.786 kb on - strand, within cbl at 858.788 kb on + strand, within cbl at 858.789 kb on - strand, within cbl at 858.789 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.865 kb on - strand, within cbl at 858.892 kb on + strand, within cbl at 858.893 kb on - strand, within cbl at 858.893 kb on - strand, within cbl at 858.900 kb on - strand, within cbl at 858.924 kb on + strand, within cbl at 858.924 kb on + strand, within cbl at 858.927 kb on + strand, within cbl at 858.928 kb on - strand, within cbl at 858.928 kb on - strand, within cbl at 858.929 kb on + strand, within cbl at 858.929 kb on + strand, within cbl at 858.932 kb on + strand, within cbl at 858.933 kb on - strand, within cbl at 858.971 kb on + strand, within cbl at 858.972 kb on - strand, within cbl at 858.986 kb on + strand, within cbl at 858.986 kb on + strand, within cbl at 859.047 kb on - strand, within cbl at 859.085 kb on + strand, within cbl at 859.086 kb on - strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.271 kb on + strand, within cbl at 859.272 kb on - strand, within cbl at 859.272 kb on - strand, within cbl at 859.274 kb on - strand, within cbl at 859.286 kb on + strand, within cbl at 859.287 kb on - strand, within cbl at 859.319 kb on + strand, within cbl
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 856,042 + ycdB MCAODC_20215 0.71 +1.6 856,046 + ycdB MCAODC_20215 0.71 +1.5 856,345 - -1.7 856,679 - +0.5 856,769 + Asn_trna MCAODC_20220 0.88 -0.7 857,102 + yeeO MCAODC_20225 0.15 -0.1 857,130 - yeeO MCAODC_20225 0.17 +2.0 857,130 - yeeO MCAODC_20225 0.17 -0.9 857,143 - yeeO MCAODC_20225 0.18 +0.7 857,172 - yeeO MCAODC_20225 0.20 +2.1 857,213 + yeeO MCAODC_20225 0.23 +2.4 857,378 + yeeO MCAODC_20225 0.34 -3.3 857,378 + yeeO MCAODC_20225 0.34 +0.4 857,414 + yeeO MCAODC_20225 0.37 +0.5 857,415 - yeeO MCAODC_20225 0.37 +2.5 857,494 - yeeO MCAODC_20225 0.42 -3.0 857,747 + yeeO MCAODC_20225 0.60 +0.8 857,748 - yeeO MCAODC_20225 0.60 -0.5 857,753 + yeeO MCAODC_20225 0.60 -0.8 857,753 + yeeO MCAODC_20225 0.60 +0.8 857,898 - yeeO MCAODC_20225 0.70 +0.5 857,957 + yeeO MCAODC_20225 0.74 -2.9 858,069 - yeeO MCAODC_20225 0.82 +1.0 858,072 - yeeO MCAODC_20225 0.82 -1.4 858,241 - +0.3 858,265 + +1.3 858,265 + -2.2 858,342 + +0.1 858,444 + -0.7 858,444 + -2.2 858,743 - cbl MCAODC_20235 0.11 -0.2 858,747 - cbl MCAODC_20235 0.11 -0.7 858,785 + cbl MCAODC_20235 0.15 +2.6 858,785 + cbl MCAODC_20235 0.15 +0.3 858,786 - cbl MCAODC_20235 0.15 +0.7 858,788 + cbl MCAODC_20235 0.16 -1.1 858,789 - cbl MCAODC_20235 0.16 +1.2 858,789 - cbl MCAODC_20235 0.16 +0.1 858,865 - cbl MCAODC_20235 0.24 -0.9 858,865 - cbl MCAODC_20235 0.24 -2.1 858,892 + cbl MCAODC_20235 0.27 +2.4 858,893 - cbl MCAODC_20235 0.27 -3.1 858,893 - cbl MCAODC_20235 0.27 -3.6 858,900 - cbl MCAODC_20235 0.27 +1.4 858,924 + cbl MCAODC_20235 0.30 +1.2 858,924 + cbl MCAODC_20235 0.30 -2.1 858,927 + cbl MCAODC_20235 0.30 +0.3 858,928 - cbl MCAODC_20235 0.30 -2.0 858,928 - cbl MCAODC_20235 0.30 +1.4 858,929 + cbl MCAODC_20235 0.30 -2.9 858,929 + cbl MCAODC_20235 0.30 +0.5 858,932 + cbl MCAODC_20235 0.31 +0.7 858,933 - cbl MCAODC_20235 0.31 -3.5 858,971 + cbl MCAODC_20235 0.35 -2.5 858,972 - cbl MCAODC_20235 0.35 +1.2 858,986 + cbl MCAODC_20235 0.36 -3.4 858,986 + cbl MCAODC_20235 0.36 -0.1 859,047 - cbl MCAODC_20235 0.43 -0.9 859,085 + cbl MCAODC_20235 0.47 +2.7 859,086 - cbl MCAODC_20235 0.47 +1.7 859,271 + cbl MCAODC_20235 0.66 -2.1 859,271 + cbl MCAODC_20235 0.66 +0.9 859,271 + cbl MCAODC_20235 0.66 -1.9 859,271 + cbl MCAODC_20235 0.66 -0.4 859,272 - cbl MCAODC_20235 0.67 +0.7 859,272 - cbl MCAODC_20235 0.67 +1.9 859,274 - cbl MCAODC_20235 0.67 +0.3 859,286 + cbl MCAODC_20235 0.68 +1.2 859,287 - cbl MCAODC_20235 0.68 -1.9 859,319 + cbl MCAODC_20235 0.72 +0.8
Or see this region's nucleotide sequence