Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsA and dsrB are separated by 43 nucleotides dsrB and yodD are separated by 162 nucleotides yodD and yedP are separated by 297 nucleotides yedP and yedQ overlap by 4 nucleotides
MCAODC_19745: rcsA - transcriptional regulator RcsA, at 777,723 to 778,346
rcsA
MCAODC_19750: dsrB - protein DsrB, at 778,390 to 778,578
dsrB
MCAODC_19755: yodD - YodD family peroxide/acid resistance protein, at 778,741 to 778,968
yodD
MCAODC_19765: yedP - mannosyl-3-phosphoglycerate phosphatase-related protein, at 779,266 to 780,081
yedP
MCAODC_19770: yedQ - cellulose biosynthesis regulator YedQ, at 780,078 to 781,772
yedQ
Position (kb) 779
780
781 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 778.287 kb on + strand at 778.413 kb on + strand, within dsrB at 778.482 kb on - strand, within dsrB at 778.609 kb on - strand at 778.672 kb on + strand at 778.672 kb on + strand at 778.673 kb on - strand at 778.676 kb on + strand at 778.676 kb on + strand at 778.676 kb on + strand at 778.677 kb on - strand at 778.759 kb on + strand at 778.891 kb on + strand, within yodD at 778.891 kb on + strand, within yodD at 778.896 kb on + strand, within yodD at 778.896 kb on + strand, within yodD at 778.897 kb on - strand, within yodD at 778.897 kb on - strand, within yodD at 778.897 kb on - strand, within yodD at 778.897 kb on - strand, within yodD at 778.932 kb on + strand, within yodD at 778.932 kb on + strand, within yodD at 778.978 kb on - strand at 779.107 kb on + strand at 779.117 kb on - strand at 779.133 kb on - strand at 779.133 kb on - strand at 779.331 kb on + strand at 779.331 kb on + strand at 779.331 kb on + strand at 779.331 kb on + strand at 779.331 kb on + strand at 779.335 kb on + strand at 779.336 kb on - strand at 779.409 kb on + strand, within yedP at 779.410 kb on - strand, within yedP at 779.463 kb on - strand, within yedP at 779.547 kb on + strand, within yedP at 779.548 kb on - strand, within yedP at 779.581 kb on - strand, within yedP at 779.581 kb on - strand, within yedP at 779.581 kb on - strand, within yedP at 779.737 kb on - strand, within yedP at 779.928 kb on - strand, within yedP at 779.936 kb on + strand, within yedP at 780.237 kb on - strand at 780.383 kb on + strand, within yedQ at 780.387 kb on + strand, within yedQ at 780.452 kb on + strand, within yedQ at 780.554 kb on - strand, within yedQ at 780.556 kb on - strand, within yedQ at 780.556 kb on - strand, within yedQ at 780.556 kb on - strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.610 kb on + strand, within yedQ at 780.611 kb on - strand, within yedQ at 780.611 kb on - strand, within yedQ at 780.611 kb on - strand, within yedQ at 780.611 kb on - strand, within yedQ at 780.665 kb on + strand, within yedQ at 780.732 kb on + strand, within yedQ at 780.738 kb on - strand, within yedQ at 780.803 kb on + strand, within yedQ at 780.928 kb on + strand, within yedQ at 780.957 kb on - strand, within yedQ at 780.986 kb on + strand, within yedQ at 780.987 kb on - strand, within yedQ at 780.987 kb on - strand, within yedQ at 781.021 kb on + strand, within yedQ at 781.027 kb on + strand, within yedQ at 781.027 kb on + strand, within yedQ at 781.028 kb on - strand, within yedQ at 781.031 kb on + strand, within yedQ at 781.031 kb on + strand, within yedQ at 781.032 kb on - strand, within yedQ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 778,287 + +2.0 778,413 + dsrB MCAODC_19750 0.12 -1.2 778,482 - dsrB MCAODC_19750 0.49 +1.5 778,609 - +0.4 778,672 + -1.3 778,672 + -2.4 778,673 - +0.6 778,676 + +1.2 778,676 + +0.9 778,676 + +0.6 778,677 - -0.5 778,759 + +0.7 778,891 + yodD MCAODC_19755 0.66 +3.0 778,891 + yodD MCAODC_19755 0.66 +1.7 778,896 + yodD MCAODC_19755 0.68 +1.6 778,896 + yodD MCAODC_19755 0.68 -0.1 778,897 - yodD MCAODC_19755 0.68 -2.3 778,897 - yodD MCAODC_19755 0.68 -1.9 778,897 - yodD MCAODC_19755 0.68 -3.1 778,897 - yodD MCAODC_19755 0.68 -2.8 778,932 + yodD MCAODC_19755 0.84 +1.3 778,932 + yodD MCAODC_19755 0.84 -1.0 778,978 - -0.9 779,107 + -2.3 779,117 - +1.9 779,133 - +0.6 779,133 - +0.7 779,331 + -0.7 779,331 + +1.7 779,331 + -1.5 779,331 + +0.4 779,331 + -1.5 779,335 + -2.0 779,336 - -0.3 779,409 + yedP MCAODC_19765 0.18 +0.3 779,410 - yedP MCAODC_19765 0.18 +0.9 779,463 - yedP MCAODC_19765 0.24 +0.1 779,547 + yedP MCAODC_19765 0.34 -3.7 779,548 - yedP MCAODC_19765 0.35 -1.6 779,581 - yedP MCAODC_19765 0.39 +0.5 779,581 - yedP MCAODC_19765 0.39 -0.9 779,581 - yedP MCAODC_19765 0.39 -2.3 779,737 - yedP MCAODC_19765 0.58 +2.2 779,928 - yedP MCAODC_19765 0.81 +2.1 779,936 + yedP MCAODC_19765 0.82 +3.0 780,237 - +1.3 780,383 + yedQ MCAODC_19770 0.18 +1.3 780,387 + yedQ MCAODC_19770 0.18 +0.2 780,452 + yedQ MCAODC_19770 0.22 +2.3 780,554 - yedQ MCAODC_19770 0.28 +0.4 780,556 - yedQ MCAODC_19770 0.28 -0.9 780,556 - yedQ MCAODC_19770 0.28 -0.1 780,556 - yedQ MCAODC_19770 0.28 +0.4 780,610 + yedQ MCAODC_19770 0.31 -1.6 780,610 + yedQ MCAODC_19770 0.31 +0.7 780,610 + yedQ MCAODC_19770 0.31 +2.5 780,610 + yedQ MCAODC_19770 0.31 -2.0 780,610 + yedQ MCAODC_19770 0.31 +0.8 780,610 + yedQ MCAODC_19770 0.31 +0.3 780,611 - yedQ MCAODC_19770 0.31 +0.7 780,611 - yedQ MCAODC_19770 0.31 -4.0 780,611 - yedQ MCAODC_19770 0.31 -0.2 780,611 - yedQ MCAODC_19770 0.31 -2.0 780,665 + yedQ MCAODC_19770 0.35 -3.5 780,732 + yedQ MCAODC_19770 0.39 -0.2 780,738 - yedQ MCAODC_19770 0.39 -1.0 780,803 + yedQ MCAODC_19770 0.43 -2.6 780,928 + yedQ MCAODC_19770 0.50 +0.2 780,957 - yedQ MCAODC_19770 0.52 +0.5 780,986 + yedQ MCAODC_19770 0.54 -2.7 780,987 - yedQ MCAODC_19770 0.54 +0.6 780,987 - yedQ MCAODC_19770 0.54 -2.4 781,021 + yedQ MCAODC_19770 0.56 -2.8 781,027 + yedQ MCAODC_19770 0.56 -0.3 781,027 + yedQ MCAODC_19770 0.56 -0.1 781,028 - yedQ MCAODC_19770 0.56 -1.7 781,031 + yedQ MCAODC_19770 0.56 +0.1 781,031 + yedQ MCAODC_19770 0.56 -2.9 781,032 - yedQ MCAODC_19770 0.56 +1.6
Or see this region's nucleotide sequence