Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and MCAODC_19385 are separated by 5 nucleotides MCAODC_19385 and MCAODC_19390 are separated by 29 nucleotides MCAODC_19390 and MCAODC_19395 are separated by 15 nucleotides
MCAODC_19380: xerD - integrase, at 722,395 to 723,396
xerD
MCAODC_19385: MCAODC_19385 - DUF2511 domain-containing protein, at 723,402 to 723,749
_19385
MCAODC_19390: MCAODC_19390 - Putative transmembrane protein, at 723,779 to 724,429
_19390
MCAODC_19395: MCAODC_19395 - transcriptional regulator, at 724,445 to 724,849
_19395
Position (kb) 723
724 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 722.438 kb on - strand at 722.470 kb on - strand at 722.470 kb on - strand at 722.488 kb on - strand at 722.561 kb on - strand, within xerD at 722.573 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.879 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.994 kb on - strand, within xerD at 723.081 kb on + strand, within xerD at 723.188 kb on + strand, within xerD at 723.188 kb on + strand, within xerD at 723.189 kb on - strand, within xerD at 723.189 kb on - strand, within xerD at 723.261 kb on + strand, within xerD at 723.284 kb on + strand, within xerD at 723.285 kb on - strand, within xerD at 723.370 kb on - strand at 723.407 kb on + strand at 723.407 kb on + strand at 723.476 kb on + strand, within MCAODC_19385 at 723.477 kb on - strand, within MCAODC_19385 at 723.501 kb on - strand, within MCAODC_19385 at 723.505 kb on - strand, within MCAODC_19385 at 723.562 kb on - strand, within MCAODC_19385 at 723.562 kb on - strand, within MCAODC_19385 at 723.570 kb on - strand, within MCAODC_19385 at 723.675 kb on - strand, within MCAODC_19385 at 723.869 kb on + strand, within MCAODC_19390 at 724.062 kb on - strand, within MCAODC_19390 at 724.074 kb on - strand, within MCAODC_19390 at 724.103 kb on - strand, within MCAODC_19390 at 724.145 kb on - strand, within MCAODC_19390 at 724.163 kb on - strand, within MCAODC_19390 at 724.211 kb on - strand, within MCAODC_19390 at 724.243 kb on + strand, within MCAODC_19390 at 724.337 kb on - strand, within MCAODC_19390 at 724.351 kb on - strand, within MCAODC_19390 at 724.351 kb on - strand, within MCAODC_19390 at 724.444 kb on - strand at 724.457 kb on - strand at 724.468 kb on - strand at 724.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 722,438 - +3.1 722,470 - -2.4 722,470 - +1.1 722,488 - +2.3 722,561 - xerD MCAODC_19380 0.17 +0.5 722,573 - xerD MCAODC_19380 0.18 +1.2 722,631 - xerD MCAODC_19380 0.24 +1.1 722,631 - xerD MCAODC_19380 0.24 +1.4 722,631 - xerD MCAODC_19380 0.24 -2.9 722,631 - xerD MCAODC_19380 0.24 -2.4 722,879 - xerD MCAODC_19380 0.48 +2.3 722,990 - xerD MCAODC_19380 0.59 +1.5 722,990 - xerD MCAODC_19380 0.59 -0.9 722,990 - xerD MCAODC_19380 0.59 +1.9 722,994 - xerD MCAODC_19380 0.60 -0.3 723,081 + xerD MCAODC_19380 0.68 +1.4 723,188 + xerD MCAODC_19380 0.79 -1.5 723,188 + xerD MCAODC_19380 0.79 +0.9 723,189 - xerD MCAODC_19380 0.79 +0.1 723,189 - xerD MCAODC_19380 0.79 +1.3 723,261 + xerD MCAODC_19380 0.86 +0.9 723,284 + xerD MCAODC_19380 0.89 -2.1 723,285 - xerD MCAODC_19380 0.89 -1.6 723,370 - -1.3 723,407 + +0.9 723,407 + -3.4 723,476 + MCAODC_19385 0.21 +0.7 723,477 - MCAODC_19385 0.22 +0.4 723,501 - MCAODC_19385 0.28 +0.1 723,505 - MCAODC_19385 0.30 +3.1 723,562 - MCAODC_19385 0.46 -2.6 723,562 - MCAODC_19385 0.46 +3.4 723,570 - MCAODC_19385 0.48 +0.8 723,675 - MCAODC_19385 0.78 -0.3 723,869 + MCAODC_19390 0.14 -3.3 724,062 - MCAODC_19390 0.43 -0.7 724,074 - MCAODC_19390 0.45 -0.7 724,103 - MCAODC_19390 0.50 +2.1 724,145 - MCAODC_19390 0.56 -1.5 724,163 - MCAODC_19390 0.59 -0.8 724,211 - MCAODC_19390 0.66 +1.7 724,243 + MCAODC_19390 0.71 -2.0 724,337 - MCAODC_19390 0.86 +0.0 724,351 - MCAODC_19390 0.88 -0.8 724,351 - MCAODC_19390 0.88 -1.2 724,444 - -1.1 724,457 - -3.0 724,468 - -3.2 724,468 - -3.6
Or see this region's nucleotide sequence