Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjE and tnp-IS629 are separated by 152 nucleotides tnp-IS629 and MCAODC_18625 overlap by 1 nucleotides MCAODC_18625 and MCAODC_18630 overlap by 26 nucleotides MCAODC_18630 and ydjF are separated by 117 nucleotides
MCAODC_18615: ydjE - Inner membrane metabolite transport protein YdjE, at 582,192 to 583,352
ydjE
MCAODC_18620: tnp-IS629 - IS3 family IS629 transposase, at 583,505 to 583,831
tnp-IS629
MCAODC_18625: MCAODC_18625 - IS3 family transposase, at 583,831 to 584,718
_18625
MCAODC_18630: MCAODC_18630 - Insertion sequence 2 OrfA protein, at 584,693 to 584,863
_18630
MCAODC_18635: ydjF - Uncharacterized HTH-type transcriptional regulator YdjF, at 584,981 to 585,739
ydjF
Position (kb) 583
584
585 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 582.891 kb on + strand, within ydjE at 582.892 kb on - strand, within ydjE at 583.086 kb on - strand, within ydjE at 583.211 kb on + strand, within ydjE at 583.220 kb on - strand, within ydjE at 583.223 kb on + strand, within ydjE at 583.223 kb on + strand, within ydjE at 583.224 kb on - strand, within ydjE at 583.276 kb on - strand at 583.276 kb on - strand at 583.416 kb on + strand at 584.777 kb on - strand, within MCAODC_18630 at 585.058 kb on - strand, within ydjF at 585.208 kb on + strand, within ydjF at 585.221 kb on - strand, within ydjF at 585.221 kb on - strand, within ydjF at 585.231 kb on - strand, within ydjF at 585.395 kb on - strand, within ydjF at 585.494 kb on - strand, within ydjF at 585.510 kb on + strand, within ydjF at 585.532 kb on - strand, within ydjF at 585.583 kb on - strand, within ydjF at 585.583 kb on - strand, within ydjF at 585.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 582,891 + ydjE MCAODC_18615 0.60 +1.6 582,892 - ydjE MCAODC_18615 0.60 +2.2 583,086 - ydjE MCAODC_18615 0.77 -4.2 583,211 + ydjE MCAODC_18615 0.88 -1.9 583,220 - ydjE MCAODC_18615 0.89 +0.7 583,223 + ydjE MCAODC_18615 0.89 -0.5 583,223 + ydjE MCAODC_18615 0.89 -2.5 583,224 - ydjE MCAODC_18615 0.89 -1.4 583,276 - +2.2 583,276 - -3.3 583,416 + -2.0 584,777 - MCAODC_18630 0.49 -1.6 585,058 - ydjF MCAODC_18635 0.10 +2.9 585,208 + ydjF MCAODC_18635 0.30 -1.4 585,221 - ydjF MCAODC_18635 0.32 +0.3 585,221 - ydjF MCAODC_18635 0.32 -0.9 585,231 - ydjF MCAODC_18635 0.33 +0.2 585,395 - ydjF MCAODC_18635 0.55 -0.2 585,494 - ydjF MCAODC_18635 0.68 +0.8 585,510 + ydjF MCAODC_18635 0.70 -1.4 585,532 - ydjF MCAODC_18635 0.73 +0.8 585,583 - ydjF MCAODC_18635 0.79 +0.8 585,583 - ydjF MCAODC_18635 0.79 +0.5 585,710 - +1.1
Or see this region's nucleotide sequence