Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntA and ydgH are separated by 523 nucleotides ydgH and ydgI are separated by 185 nucleotides
MCAODC_17720: pntA - Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha, at 405,441 to 406,973
pntA
MCAODC_17725: ydgH - DUF1471 domain-containing protein, at 407,497 to 408,441
ydgH
MCAODC_17730: ydgI - Putative arginine/ornithine antiporter, at 408,627 to 410,009
ydgI
Position (kb) 407
408
409 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 406.568 kb on + strand, within pntA at 406.585 kb on - strand, within pntA at 406.613 kb on + strand, within pntA at 406.746 kb on + strand, within pntA at 406.747 kb on - strand, within pntA at 406.747 kb on - strand, within pntA at 407.042 kb on + strand at 407.043 kb on - strand at 407.049 kb on + strand at 407.050 kb on - strand at 407.106 kb on + strand at 407.195 kb on + strand at 407.195 kb on + strand at 407.196 kb on - strand at 407.207 kb on + strand at 407.217 kb on + strand at 407.218 kb on - strand at 407.291 kb on + strand at 407.313 kb on + strand at 407.313 kb on + strand at 407.313 kb on + strand at 407.339 kb on - strand at 407.377 kb on + strand at 407.390 kb on - strand at 407.452 kb on + strand at 407.452 kb on + strand at 407.506 kb on - strand at 407.634 kb on + strand, within ydgH at 407.640 kb on + strand, within ydgH at 407.640 kb on + strand, within ydgH at 407.764 kb on - strand, within ydgH at 407.808 kb on + strand, within ydgH at 407.809 kb on - strand, within ydgH at 407.944 kb on + strand, within ydgH at 407.953 kb on + strand, within ydgH at 407.953 kb on + strand, within ydgH at 407.954 kb on - strand, within ydgH at 407.998 kb on - strand, within ydgH at 408.000 kb on + strand, within ydgH at 408.307 kb on + strand, within ydgH at 408.307 kb on + strand, within ydgH at 408.307 kb on + strand, within ydgH at 408.308 kb on - strand, within ydgH at 408.405 kb on + strand at 408.405 kb on + strand at 408.406 kb on - strand at 408.435 kb on + strand at 408.435 kb on + strand at 408.435 kb on + strand at 408.440 kb on - strand at 408.440 kb on - strand at 408.457 kb on + strand at 408.457 kb on + strand at 408.457 kb on + strand at 408.458 kb on - strand at 408.460 kb on - strand at 408.491 kb on - strand at 408.601 kb on + strand at 408.612 kb on - strand at 408.758 kb on + strand at 408.762 kb on - strand at 408.842 kb on + strand, within ydgI at 408.846 kb on + strand, within ydgI at 408.846 kb on + strand, within ydgI at 408.871 kb on + strand, within ydgI at 409.001 kb on + strand, within ydgI at 409.001 kb on + strand, within ydgI at 409.004 kb on + strand, within ydgI at 409.036 kb on + strand, within ydgI at 409.207 kb on + strand, within ydgI at 409.207 kb on + strand, within ydgI
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 406,568 + pntA MCAODC_17720 0.74 -2.1 406,585 - pntA MCAODC_17720 0.75 +0.6 406,613 + pntA MCAODC_17720 0.76 +0.7 406,746 + pntA MCAODC_17720 0.85 +0.3 406,747 - pntA MCAODC_17720 0.85 +0.3 406,747 - pntA MCAODC_17720 0.85 -3.6 407,042 + +1.5 407,043 - +0.6 407,049 + -1.4 407,050 - -0.4 407,106 + +2.1 407,195 + -0.9 407,195 + +0.4 407,196 - -3.0 407,207 + -0.9 407,217 + +0.6 407,218 - -3.2 407,291 + -3.0 407,313 + -0.2 407,313 + +0.3 407,313 + -1.5 407,339 - -4.9 407,377 + -1.1 407,390 - -0.4 407,452 + -1.6 407,452 + +0.9 407,506 - -1.3 407,634 + ydgH MCAODC_17725 0.14 -2.7 407,640 + ydgH MCAODC_17725 0.15 +1.1 407,640 + ydgH MCAODC_17725 0.15 +0.3 407,764 - ydgH MCAODC_17725 0.28 -0.9 407,808 + ydgH MCAODC_17725 0.33 +0.9 407,809 - ydgH MCAODC_17725 0.33 +2.3 407,944 + ydgH MCAODC_17725 0.47 -0.3 407,953 + ydgH MCAODC_17725 0.48 +2.1 407,953 + ydgH MCAODC_17725 0.48 -4.1 407,954 - ydgH MCAODC_17725 0.48 +0.5 407,998 - ydgH MCAODC_17725 0.53 +0.2 408,000 + ydgH MCAODC_17725 0.53 -0.5 408,307 + ydgH MCAODC_17725 0.86 +0.0 408,307 + ydgH MCAODC_17725 0.86 -2.4 408,307 + ydgH MCAODC_17725 0.86 -0.8 408,308 - ydgH MCAODC_17725 0.86 -2.6 408,405 + -2.0 408,405 + -1.3 408,406 - +2.5 408,435 + +0.9 408,435 + -0.1 408,435 + +0.0 408,440 - -1.0 408,440 - -1.9 408,457 + -3.9 408,457 + -0.1 408,457 + +0.5 408,458 - +0.9 408,460 - -3.8 408,491 - -0.6 408,601 + +0.7 408,612 - +1.4 408,758 + -1.1 408,762 - +1.4 408,842 + ydgI MCAODC_17730 0.16 -1.1 408,846 + ydgI MCAODC_17730 0.16 -4.3 408,846 + ydgI MCAODC_17730 0.16 -2.8 408,871 + ydgI MCAODC_17730 0.18 +0.9 409,001 + ydgI MCAODC_17730 0.27 -2.0 409,001 + ydgI MCAODC_17730 0.27 -2.0 409,004 + ydgI MCAODC_17730 0.27 -0.3 409,036 + ydgI MCAODC_17730 0.30 -1.0 409,207 + ydgI MCAODC_17730 0.42 +0.2 409,207 + ydgI MCAODC_17730 0.42 -0.9
Or see this region's nucleotide sequence