Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntB and pntA are separated by 10 nucleotides pntA and ydgH are separated by 523 nucleotides
MCAODC_17715: pntB - Re/Si-specific NAD(P)(+) transhydrogenase subunit beta, at 404,042 to 405,430
pntB
MCAODC_17720: pntA - Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha, at 405,441 to 406,973
pntA
MCAODC_17725: ydgH - DUF1471 domain-containing protein, at 407,497 to 408,441
ydgH
Position (kb) 405
406
407 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 404.488 kb on - strand, within pntB at 404.656 kb on + strand, within pntB at 404.832 kb on - strand, within pntB at 404.901 kb on - strand, within pntB at 404.901 kb on - strand, within pntB at 405.115 kb on - strand, within pntB at 405.403 kb on - strand at 405.403 kb on - strand at 405.403 kb on - strand at 405.417 kb on - strand at 405.430 kb on + strand at 405.431 kb on - strand at 405.431 kb on - strand at 405.431 kb on - strand at 405.498 kb on - strand at 405.513 kb on - strand at 405.531 kb on - strand at 405.623 kb on + strand, within pntA at 405.624 kb on - strand, within pntA at 405.656 kb on - strand, within pntA at 405.656 kb on - strand, within pntA at 405.876 kb on - strand, within pntA at 406.040 kb on - strand, within pntA at 406.094 kb on - strand, within pntA at 406.094 kb on - strand, within pntA at 406.177 kb on - strand, within pntA at 406.177 kb on - strand, within pntA at 406.326 kb on - strand, within pntA at 406.568 kb on + strand, within pntA at 406.585 kb on - strand, within pntA at 406.613 kb on + strand, within pntA at 406.746 kb on + strand, within pntA at 406.747 kb on - strand, within pntA at 406.747 kb on - strand, within pntA at 407.042 kb on + strand at 407.043 kb on - strand at 407.049 kb on + strand at 407.050 kb on - strand at 407.106 kb on + strand at 407.195 kb on + strand at 407.195 kb on + strand at 407.196 kb on - strand at 407.207 kb on + strand at 407.217 kb on + strand at 407.218 kb on - strand at 407.291 kb on + strand at 407.313 kb on + strand at 407.313 kb on + strand at 407.313 kb on + strand at 407.339 kb on - strand at 407.377 kb on + strand at 407.390 kb on - strand at 407.452 kb on + strand at 407.452 kb on + strand at 407.506 kb on - strand at 407.634 kb on + strand, within ydgH at 407.640 kb on + strand, within ydgH at 407.640 kb on + strand, within ydgH at 407.764 kb on - strand, within ydgH at 407.808 kb on + strand, within ydgH at 407.809 kb on - strand, within ydgH at 407.944 kb on + strand, within ydgH at 407.953 kb on + strand, within ydgH at 407.953 kb on + strand, within ydgH at 407.954 kb on - strand, within ydgH
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 404,488 - pntB MCAODC_17715 0.32 -4.3 404,656 + pntB MCAODC_17715 0.44 -1.6 404,832 - pntB MCAODC_17715 0.57 -0.7 404,901 - pntB MCAODC_17715 0.62 -1.3 404,901 - pntB MCAODC_17715 0.62 -1.9 405,115 - pntB MCAODC_17715 0.77 -0.8 405,403 - +1.1 405,403 - -0.2 405,403 - +0.4 405,417 - +0.0 405,430 + -0.3 405,431 - -0.2 405,431 - +0.7 405,431 - -0.8 405,498 - -1.7 405,513 - +0.9 405,531 - -3.0 405,623 + pntA MCAODC_17720 0.12 +1.7 405,624 - pntA MCAODC_17720 0.12 -0.9 405,656 - pntA MCAODC_17720 0.14 +2.1 405,656 - pntA MCAODC_17720 0.14 -1.0 405,876 - pntA MCAODC_17720 0.28 -0.1 406,040 - pntA MCAODC_17720 0.39 +0.4 406,094 - pntA MCAODC_17720 0.43 +0.5 406,094 - pntA MCAODC_17720 0.43 +0.1 406,177 - pntA MCAODC_17720 0.48 +1.2 406,177 - pntA MCAODC_17720 0.48 +1.1 406,326 - pntA MCAODC_17720 0.58 -2.8 406,568 + pntA MCAODC_17720 0.74 -2.1 406,585 - pntA MCAODC_17720 0.75 +0.6 406,613 + pntA MCAODC_17720 0.76 +0.7 406,746 + pntA MCAODC_17720 0.85 +0.3 406,747 - pntA MCAODC_17720 0.85 +0.3 406,747 - pntA MCAODC_17720 0.85 -3.6 407,042 + +1.5 407,043 - +0.6 407,049 + -1.4 407,050 - -0.4 407,106 + +2.1 407,195 + -0.9 407,195 + +0.4 407,196 - -3.0 407,207 + -0.9 407,217 + +0.6 407,218 - -3.2 407,291 + -3.0 407,313 + -0.2 407,313 + +0.3 407,313 + -1.5 407,339 - -4.9 407,377 + -1.1 407,390 - -0.4 407,452 + -1.6 407,452 + +0.9 407,506 - -1.3 407,634 + ydgH MCAODC_17725 0.14 -2.7 407,640 + ydgH MCAODC_17725 0.15 +1.1 407,640 + ydgH MCAODC_17725 0.15 +0.3 407,764 - ydgH MCAODC_17725 0.28 -0.9 407,808 + ydgH MCAODC_17725 0.33 +0.9 407,809 - ydgH MCAODC_17725 0.33 +2.3 407,944 + ydgH MCAODC_17725 0.47 -0.3 407,953 + ydgH MCAODC_17725 0.48 +2.1 407,953 + ydgH MCAODC_17725 0.48 -4.1 407,954 - ydgH MCAODC_17725 0.48 +0.5
Or see this region's nucleotide sequence