Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_17595 and MCAODC_17600 are separated by 34 nucleotides MCAODC_17600 and MCAODC_17605 are separated by 500 nucleotides MCAODC_17605 and ynfA are separated by 205 nucleotides ynfA and ynfB are separated by 134 nucleotides
MCAODC_17595: MCAODC_17595 - DNA-binding protein, at 383,193 to 383,429
_17595
MCAODC_17600: MCAODC_17600 - Integrase, cryptic prophage CP-933P, at 383,464 to 383,619
_17600
MCAODC_17605: MCAODC_17605 - Starvation-sensing protein, at 384,120 to 384,212
_17605
MCAODC_17610: ynfA - YnfA family protein, at 384,418 to 384,744
ynfA
MCAODC_17615: ynfB - DUF1283 domain-containing protein, at 384,879 to 385,220
ynfB
Position (kb) 384
385 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 383.167 kb on + strand at 383.188 kb on + strand at 383.309 kb on + strand, within MCAODC_17595 at 383.309 kb on + strand, within MCAODC_17595 at 383.309 kb on + strand, within MCAODC_17595 at 383.309 kb on + strand, within MCAODC_17595 at 383.309 kb on + strand, within MCAODC_17595 at 383.310 kb on - strand, within MCAODC_17595 at 383.509 kb on - strand, within MCAODC_17600 at 383.521 kb on + strand, within MCAODC_17600 at 383.523 kb on + strand, within MCAODC_17600 at 383.638 kb on - strand at 383.698 kb on + strand at 383.699 kb on - strand at 383.901 kb on + strand at 383.938 kb on + strand at 383.946 kb on + strand at 384.128 kb on + strand at 384.128 kb on + strand at 384.128 kb on + strand at 384.129 kb on - strand at 384.129 kb on - strand at 384.132 kb on - strand, within MCAODC_17605 at 384.268 kb on + strand at 384.268 kb on + strand at 384.273 kb on + strand at 384.274 kb on - strand at 384.276 kb on - strand at 384.321 kb on + strand at 384.323 kb on + strand at 384.489 kb on - strand, within ynfA at 384.545 kb on + strand, within ynfA at 384.545 kb on + strand, within ynfA at 384.546 kb on - strand, within ynfA at 384.557 kb on + strand, within ynfA at 384.558 kb on - strand, within ynfA at 384.558 kb on - strand, within ynfA at 384.756 kb on - strand at 384.768 kb on + strand at 384.768 kb on + strand at 384.769 kb on - strand at 384.769 kb on - strand at 384.769 kb on - strand at 384.774 kb on + strand at 384.786 kb on + strand at 384.786 kb on + strand at 384.787 kb on - strand at 384.787 kb on - strand at 384.787 kb on - strand at 384.790 kb on - strand at 384.800 kb on + strand at 384.800 kb on + strand at 384.801 kb on - strand at 384.842 kb on + strand at 384.843 kb on - strand at 384.864 kb on + strand at 385.127 kb on + strand, within ynfB at 385.127 kb on + strand, within ynfB at 385.172 kb on + strand, within ynfB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 383,167 + +0.7 383,188 + +0.5 383,309 + MCAODC_17595 0.49 -0.5 383,309 + MCAODC_17595 0.49 +0.3 383,309 + MCAODC_17595 0.49 +2.0 383,309 + MCAODC_17595 0.49 -2.4 383,309 + MCAODC_17595 0.49 -3.2 383,310 - MCAODC_17595 0.49 -1.1 383,509 - MCAODC_17600 0.29 +1.6 383,521 + MCAODC_17600 0.37 +1.5 383,523 + MCAODC_17600 0.38 +0.9 383,638 - +0.5 383,698 + -2.0 383,699 - -2.4 383,901 + +0.2 383,938 + -1.9 383,946 + -1.3 384,128 + -1.7 384,128 + -0.4 384,128 + +0.6 384,129 - +0.9 384,129 - +0.1 384,132 - MCAODC_17605 0.13 +0.7 384,268 + -3.0 384,268 + -4.0 384,273 + +0.5 384,274 - +3.0 384,276 - -0.3 384,321 + -0.5 384,323 + -2.7 384,489 - ynfA MCAODC_17610 0.22 -2.0 384,545 + ynfA MCAODC_17610 0.39 -0.3 384,545 + ynfA MCAODC_17610 0.39 -0.5 384,546 - ynfA MCAODC_17610 0.39 +1.7 384,557 + ynfA MCAODC_17610 0.43 +2.8 384,558 - ynfA MCAODC_17610 0.43 -3.1 384,558 - ynfA MCAODC_17610 0.43 -1.4 384,756 - +0.1 384,768 + -2.5 384,768 + -0.2 384,769 - +2.8 384,769 - -4.9 384,769 - -2.0 384,774 + +2.8 384,786 + -0.7 384,786 + -2.0 384,787 - -1.4 384,787 - +0.8 384,787 - +0.3 384,790 - -2.5 384,800 + -2.7 384,800 + +1.0 384,801 - -1.1 384,842 + +1.0 384,843 - +0.4 384,864 + +0.2 385,127 + ynfB MCAODC_17615 0.73 -2.0 385,127 + ynfB MCAODC_17615 0.73 +1.0 385,172 + ynfB MCAODC_17615 0.86 -2.6
Or see this region's nucleotide sequence