Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeE and pspE are separated by 40 nucleotides pspE and ftrA are separated by 94 nucleotides ftrA and mgtS are separated by 107 nucleotides mgtS and dgcZ are separated by 218 nucleotides
MCAODC_16865: ydeE - efflux MFS transporter YdeE, at 283,317 to 284,504
ydeE
MCAODC_16870: pspE - rhodanese, at 284,545 to 284,940
pspE
MCAODC_16875: ftrA - transcriptional regulator FtrA, at 285,035 to 286,006
ftrA
MCAODC_16880: mgtS - protein MgtS, at 286,114 to 286,209
mgtS
MCAODC_16890: dgcZ - diguanylate cyclase DgcZ, at 286,428 to 287,318
dgcZ
Position (kb) 285
286
287 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 284.213 kb on - strand, within ydeE at 284.213 kb on - strand, within ydeE at 284.237 kb on - strand, within ydeE at 284.241 kb on - strand, within ydeE at 284.255 kb on - strand, within ydeE at 284.274 kb on + strand, within ydeE at 284.274 kb on + strand, within ydeE at 284.277 kb on - strand, within ydeE at 284.291 kb on + strand, within ydeE at 284.338 kb on + strand, within ydeE at 284.338 kb on + strand, within ydeE at 284.369 kb on - strand, within ydeE at 284.387 kb on + strand at 284.387 kb on + strand at 284.659 kb on - strand, within pspE at 284.690 kb on - strand, within pspE at 284.690 kb on - strand, within pspE at 284.690 kb on - strand, within pspE at 284.933 kb on + strand at 284.934 kb on - strand at 285.045 kb on + strand at 285.046 kb on - strand at 285.075 kb on - strand at 285.084 kb on + strand at 285.084 kb on + strand at 285.084 kb on + strand at 285.085 kb on - strand at 285.148 kb on + strand, within ftrA at 285.192 kb on + strand, within ftrA at 285.262 kb on + strand, within ftrA at 285.265 kb on + strand, within ftrA at 285.286 kb on - strand, within ftrA at 285.488 kb on + strand, within ftrA at 285.553 kb on + strand, within ftrA at 285.688 kb on - strand, within ftrA at 285.733 kb on + strand, within ftrA at 286.042 kb on + strand at 286.046 kb on - strand at 286.046 kb on - strand at 286.112 kb on + strand at 286.112 kb on + strand at 286.113 kb on - strand at 286.125 kb on + strand, within mgtS at 286.145 kb on + strand, within mgtS at 286.220 kb on + strand at 286.220 kb on + strand at 286.257 kb on + strand at 286.490 kb on + strand at 286.586 kb on + strand, within dgcZ at 286.624 kb on - strand, within dgcZ at 286.765 kb on - strand, within dgcZ at 286.768 kb on - strand, within dgcZ at 286.768 kb on - strand, within dgcZ at 286.837 kb on - strand, within dgcZ at 286.960 kb on + strand, within dgcZ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 284,213 - ydeE MCAODC_16865 0.75 +2.5 284,213 - ydeE MCAODC_16865 0.75 -1.5 284,237 - ydeE MCAODC_16865 0.77 -0.6 284,241 - ydeE MCAODC_16865 0.78 +1.3 284,255 - ydeE MCAODC_16865 0.79 -3.0 284,274 + ydeE MCAODC_16865 0.81 -0.8 284,274 + ydeE MCAODC_16865 0.81 -0.1 284,277 - ydeE MCAODC_16865 0.81 +1.4 284,291 + ydeE MCAODC_16865 0.82 +2.0 284,338 + ydeE MCAODC_16865 0.86 +1.0 284,338 + ydeE MCAODC_16865 0.86 -2.7 284,369 - ydeE MCAODC_16865 0.89 +0.9 284,387 + -3.4 284,387 + -1.7 284,659 - pspE MCAODC_16870 0.29 -1.0 284,690 - pspE MCAODC_16870 0.37 +0.7 284,690 - pspE MCAODC_16870 0.37 +1.2 284,690 - pspE MCAODC_16870 0.37 -1.7 284,933 + -2.0 284,934 - +0.5 285,045 + -0.3 285,046 - -3.9 285,075 - -0.1 285,084 + +1.8 285,084 + +0.0 285,084 + -1.9 285,085 - +1.2 285,148 + ftrA MCAODC_16875 0.12 -1.1 285,192 + ftrA MCAODC_16875 0.16 +1.2 285,262 + ftrA MCAODC_16875 0.23 -2.4 285,265 + ftrA MCAODC_16875 0.24 +1.0 285,286 - ftrA MCAODC_16875 0.26 +2.9 285,488 + ftrA MCAODC_16875 0.47 -3.8 285,553 + ftrA MCAODC_16875 0.53 -0.1 285,688 - ftrA MCAODC_16875 0.67 -2.0 285,733 + ftrA MCAODC_16875 0.72 +2.7 286,042 + -1.0 286,046 - -1.2 286,046 - -1.0 286,112 + +0.3 286,112 + -2.6 286,113 - -1.3 286,125 + mgtS MCAODC_16880 0.11 +0.8 286,145 + mgtS MCAODC_16880 0.32 -1.6 286,220 + +0.1 286,220 + -3.1 286,257 + +0.3 286,490 + +0.4 286,586 + dgcZ MCAODC_16890 0.18 -1.1 286,624 - dgcZ MCAODC_16890 0.22 -1.1 286,765 - dgcZ MCAODC_16890 0.38 -0.6 286,768 - dgcZ MCAODC_16890 0.38 -0.0 286,768 - dgcZ MCAODC_16890 0.38 -1.7 286,837 - dgcZ MCAODC_16890 0.46 +2.9 286,960 + dgcZ MCAODC_16890 0.60 +1.4
Or see this region's nucleotide sequence