Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcA and yncP are separated by 25 nucleotides yncP and MCAODC_16265 are separated by 17 nucleotides MCAODC_16265 and trg are separated by 340 nucleotides trg and ydcI are separated by 37 nucleotides
MCAODC_16255: ydcA - Uncharacterized protein YdcA, at 146,749 to 146,922
ydcA
MCAODC_16260: yncP - Protein YncP, at 146,948 to 147,016
yncP
MCAODC_16265: MCAODC_16265 - Regulatory protein mokB, at 147,034 to 147,201
_16265
MCAODC_16275: trg - methyl-accepting chemotaxis protein Trg, at 147,542 to 149,182
trg
MCAODC_16280: ydcI - Uncharacterized HTH-type transcriptional regulator YdcI, at 149,220 to 150,143
ydcI
Position (kb) 147
148
149
150 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 146.569 kb on - strand at 146.588 kb on + strand at 146.589 kb on - strand at 146.702 kb on + strand at 146.702 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.746 kb on + strand at 146.747 kb on - strand at 146.798 kb on - strand, within ydcA at 146.853 kb on + strand, within ydcA at 146.853 kb on + strand, within ydcA at 146.890 kb on - strand, within ydcA at 146.996 kb on - strand, within yncP at 147.098 kb on + strand, within MCAODC_16265 at 147.099 kb on - strand, within MCAODC_16265 at 147.219 kb on + strand at 147.301 kb on - strand at 147.301 kb on - strand at 147.437 kb on - strand at 147.477 kb on - strand at 147.480 kb on + strand at 147.481 kb on - strand at 147.481 kb on - strand at 147.481 kb on - strand at 147.628 kb on - strand at 147.628 kb on - strand at 147.628 kb on - strand at 147.642 kb on + strand at 147.769 kb on + strand, within trg at 147.769 kb on + strand, within trg at 147.950 kb on + strand, within trg at 147.951 kb on - strand, within trg at 147.970 kb on + strand, within trg at 147.971 kb on - strand, within trg at 147.971 kb on - strand, within trg at 148.053 kb on - strand, within trg at 148.054 kb on + strand, within trg at 148.078 kb on + strand, within trg at 148.083 kb on + strand, within trg at 148.087 kb on + strand, within trg at 148.198 kb on + strand, within trg at 148.198 kb on + strand, within trg at 148.198 kb on + strand, within trg at 148.206 kb on + strand, within trg at 148.320 kb on + strand, within trg at 148.332 kb on + strand, within trg at 148.397 kb on + strand, within trg at 148.397 kb on + strand, within trg at 148.605 kb on + strand, within trg at 148.927 kb on + strand, within trg at 148.928 kb on - strand, within trg at 149.088 kb on + strand at 149.106 kb on + strand at 149.164 kb on + strand at 149.287 kb on - strand at 149.299 kb on - strand at 149.402 kb on - strand, within ydcI at 149.402 kb on - strand, within ydcI at 149.402 kb on - strand, within ydcI at 149.402 kb on - strand, within ydcI at 149.487 kb on + strand, within ydcI at 149.488 kb on - strand, within ydcI at 149.488 kb on - strand, within ydcI at 149.491 kb on - strand, within ydcI at 149.562 kb on + strand, within ydcI at 149.568 kb on + strand, within ydcI at 149.785 kb on - strand, within ydcI at 149.785 kb on - strand, within ydcI at 149.816 kb on + strand, within ydcI at 149.821 kb on - strand, within ydcI at 149.846 kb on + strand, within ydcI at 149.847 kb on - strand, within ydcI at 149.901 kb on + strand, within ydcI at 149.943 kb on - strand, within ydcI at 150.085 kb on - strand at 150.085 kb on - strand at 150.093 kb on - strand at 150.093 kb on - strand at 150.131 kb on + strand at 150.153 kb on + strand at 150.154 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 146,569 - -0.2 146,588 + +1.0 146,589 - -2.0 146,702 + +0.2 146,702 + +0.8 146,722 + -1.7 146,722 + -0.3 146,722 + -0.5 146,746 + +1.1 146,747 - +1.6 146,798 - ydcA MCAODC_16255 0.28 -1.2 146,853 + ydcA MCAODC_16255 0.60 +0.2 146,853 + ydcA MCAODC_16255 0.60 +1.7 146,890 - ydcA MCAODC_16255 0.81 -1.6 146,996 - yncP MCAODC_16260 0.70 +1.4 147,098 + MCAODC_16265 0.38 -0.7 147,099 - MCAODC_16265 0.39 -1.1 147,219 + +2.2 147,301 - +0.7 147,301 - -0.7 147,437 - -2.1 147,477 - -0.0 147,480 + -0.0 147,481 - +0.5 147,481 - -1.3 147,481 - +0.1 147,628 - +1.4 147,628 - +0.3 147,628 - -2.1 147,642 + -1.1 147,769 + trg MCAODC_16275 0.14 -0.2 147,769 + trg MCAODC_16275 0.14 -1.6 147,950 + trg MCAODC_16275 0.25 +1.9 147,951 - trg MCAODC_16275 0.25 +3.7 147,970 + trg MCAODC_16275 0.26 +0.7 147,971 - trg MCAODC_16275 0.26 -1.6 147,971 - trg MCAODC_16275 0.26 +1.0 148,053 - trg MCAODC_16275 0.31 -0.3 148,054 + trg MCAODC_16275 0.31 +0.4 148,078 + trg MCAODC_16275 0.33 -2.4 148,083 + trg MCAODC_16275 0.33 +0.0 148,087 + trg MCAODC_16275 0.33 -2.6 148,198 + trg MCAODC_16275 0.40 +0.1 148,198 + trg MCAODC_16275 0.40 -0.8 148,198 + trg MCAODC_16275 0.40 -0.6 148,206 + trg MCAODC_16275 0.40 +1.9 148,320 + trg MCAODC_16275 0.47 -3.9 148,332 + trg MCAODC_16275 0.48 -1.7 148,397 + trg MCAODC_16275 0.52 -1.4 148,397 + trg MCAODC_16275 0.52 -0.2 148,605 + trg MCAODC_16275 0.65 -3.4 148,927 + trg MCAODC_16275 0.84 -3.7 148,928 - trg MCAODC_16275 0.84 +2.1 149,088 + -2.3 149,106 + -1.4 149,164 + -1.4 149,287 - -0.2 149,299 - +1.2 149,402 - ydcI MCAODC_16280 0.20 +2.0 149,402 - ydcI MCAODC_16280 0.20 -2.4 149,402 - ydcI MCAODC_16280 0.20 +0.7 149,402 - ydcI MCAODC_16280 0.20 +0.7 149,487 + ydcI MCAODC_16280 0.29 +1.7 149,488 - ydcI MCAODC_16280 0.29 +1.1 149,488 - ydcI MCAODC_16280 0.29 -1.2 149,491 - ydcI MCAODC_16280 0.29 +1.4 149,562 + ydcI MCAODC_16280 0.37 +1.7 149,568 + ydcI MCAODC_16280 0.38 +0.1 149,785 - ydcI MCAODC_16280 0.61 +2.3 149,785 - ydcI MCAODC_16280 0.61 -0.3 149,816 + ydcI MCAODC_16280 0.65 +0.8 149,821 - ydcI MCAODC_16280 0.65 +1.0 149,846 + ydcI MCAODC_16280 0.68 +1.4 149,847 - ydcI MCAODC_16280 0.68 -0.2 149,901 + ydcI MCAODC_16280 0.74 +0.4 149,943 - ydcI MCAODC_16280 0.78 +0.1 150,085 - -3.9 150,085 - -0.6 150,093 - -1.3 150,093 - -2.1 150,131 + -0.3 150,153 + -2.1 150,154 - -0.3
Or see this region's nucleotide sequence