Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaA and lrhA are separated by 919 nucleotides lrhA and MCAODC_13875 are separated by 203 nucleotides MCAODC_13875 and nuoA are separated by 262 nucleotides
MCAODC_13865: alaA - alanine transaminase AlaA, at 2,712,772 to 2,713,989
alaA
MCAODC_13870: lrhA - transcriptional regulator LrhA, at 2,714,909 to 2,715,847
lrhA
MCAODC_13875: MCAODC_13875 - hypothetical protein, at 2,716,051 to 2,716,215
_13875
MCAODC_13880: nuoA - NADH-quinone oxidoreductase subunit NuoA, at 2,716,478 to 2,716,921
nuoA
Position (kb)
2714
2715
2716 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2713.946 kb on + strand at 2713.960 kb on + strand at 2714.042 kb on + strand at 2714.052 kb on + strand at 2714.053 kb on - strand at 2714.053 kb on - strand at 2714.125 kb on + strand at 2714.133 kb on + strand at 2714.137 kb on - strand at 2714.179 kb on - strand at 2714.235 kb on - strand at 2714.403 kb on - strand at 2714.405 kb on + strand at 2714.443 kb on - strand at 2714.446 kb on - strand at 2714.462 kb on + strand at 2714.467 kb on + strand at 2714.467 kb on + strand at 2714.467 kb on + strand at 2714.472 kb on + strand at 2714.472 kb on + strand at 2714.473 kb on - strand at 2714.526 kb on + strand at 2714.539 kb on + strand at 2714.539 kb on + strand at 2714.539 kb on + strand at 2714.539 kb on + strand at 2714.540 kb on - strand at 2714.642 kb on + strand at 2714.649 kb on - strand at 2714.650 kb on + strand at 2714.650 kb on + strand at 2714.651 kb on - strand at 2714.669 kb on + strand at 2714.673 kb on + strand at 2714.673 kb on + strand at 2714.692 kb on + strand at 2714.693 kb on - strand at 2714.695 kb on + strand at 2714.695 kb on + strand at 2714.696 kb on - strand at 2714.717 kb on - strand at 2714.719 kb on - strand at 2714.719 kb on - strand at 2714.730 kb on + strand at 2714.770 kb on + strand at 2714.783 kb on - strand at 2714.835 kb on + strand at 2714.836 kb on - strand at 2714.836 kb on - strand at 2714.906 kb on + strand at 2714.906 kb on + strand at 2714.907 kb on - strand at 2714.907 kb on - strand at 2714.907 kb on - strand at 2714.908 kb on + strand at 2714.908 kb on + strand at 2714.908 kb on + strand at 2714.908 kb on + strand at 2714.909 kb on - strand at 2714.909 kb on - strand at 2714.913 kb on + strand at 2714.913 kb on + strand at 2714.935 kb on + strand at 2715.193 kb on + strand, within lrhA at 2715.210 kb on + strand, within lrhA at 2715.249 kb on + strand, within lrhA at 2715.249 kb on + strand, within lrhA at 2715.250 kb on - strand, within lrhA at 2715.250 kb on - strand, within lrhA at 2715.316 kb on + strand, within lrhA at 2715.316 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.323 kb on + strand, within lrhA at 2715.324 kb on - strand, within lrhA at 2715.324 kb on - strand, within lrhA at 2715.324 kb on - strand, within lrhA at 2715.443 kb on + strand, within lrhA at 2715.443 kb on + strand, within lrhA at 2715.444 kb on - strand, within lrhA at 2715.447 kb on + strand, within lrhA at 2715.448 kb on - strand, within lrhA at 2715.448 kb on - strand, within lrhA at 2715.448 kb on - strand, within lrhA at 2715.477 kb on + strand, within lrhA at 2715.477 kb on + strand, within lrhA at 2715.508 kb on + strand, within lrhA at 2715.526 kb on + strand, within lrhA at 2715.527 kb on - strand, within lrhA at 2715.527 kb on - strand, within lrhA at 2715.538 kb on + strand, within lrhA at 2715.538 kb on + strand, within lrhA at 2715.557 kb on + strand, within lrhA at 2715.557 kb on + strand, within lrhA at 2715.557 kb on + strand, within lrhA at 2715.666 kb on + strand, within lrhA at 2715.666 kb on + strand, within lrhA at 2715.675 kb on + strand, within lrhA at 2715.675 kb on + strand, within lrhA at 2715.699 kb on - strand, within lrhA at 2715.710 kb on + strand, within lrhA at 2715.725 kb on - strand, within lrhA at 2715.728 kb on - strand, within lrhA at 2715.728 kb on - strand, within lrhA at 2715.749 kb on + strand, within lrhA at 2715.750 kb on - strand, within lrhA at 2715.786 kb on - strand at 2715.795 kb on + strand at 2715.796 kb on - strand at 2715.859 kb on + strand at 2715.863 kb on + strand at 2715.864 kb on - strand at 2715.878 kb on + strand at 2715.910 kb on - strand at 2716.017 kb on - strand at 2716.042 kb on - strand at 2716.086 kb on + strand, within MCAODC_13875 at 2716.123 kb on - strand, within MCAODC_13875 at 2716.166 kb on + strand, within MCAODC_13875 at 2716.167 kb on - strand, within MCAODC_13875 at 2716.167 kb on - strand, within MCAODC_13875 at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.217 kb on + strand at 2716.218 kb on - strand at 2716.218 kb on - strand at 2716.218 kb on - strand at 2716.259 kb on + strand at 2716.259 kb on + strand at 2716.259 kb on + strand at 2716.260 kb on - strand at 2716.290 kb on + strand at 2716.362 kb on + strand at 2716.472 kb on + strand at 2716.554 kb on + strand, within nuoA at 2716.554 kb on + strand, within nuoA at 2716.554 kb on + strand, within nuoA at 2716.555 kb on - strand, within nuoA at 2716.555 kb on - strand, within nuoA at 2716.663 kb on - strand, within nuoA at 2716.663 kb on - strand, within nuoA at 2716.727 kb on + strand, within nuoA at 2716.727 kb on + strand, within nuoA at 2716.824 kb on - strand, within nuoA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,713,946 + -0.1 2,713,960 + -0.5 2,714,042 + -2.4 2,714,052 + +0.9 2,714,053 - -2.6 2,714,053 - +0.5 2,714,125 + +0.6 2,714,133 + +1.5 2,714,137 - +1.5 2,714,179 - -3.9 2,714,235 - +1.2 2,714,403 - +2.4 2,714,405 + +0.7 2,714,443 - -0.5 2,714,446 - +1.6 2,714,462 + +0.4 2,714,467 + +0.2 2,714,467 + +1.5 2,714,467 + +1.0 2,714,472 + -0.0 2,714,472 + +0.7 2,714,473 - -3.0 2,714,526 + -1.3 2,714,539 + +1.3 2,714,539 + +1.6 2,714,539 + +0.1 2,714,539 + +1.2 2,714,540 - +0.4 2,714,642 + +0.3 2,714,649 - -0.1 2,714,650 + +0.3 2,714,650 + -0.1 2,714,651 - +1.1 2,714,669 + +0.9 2,714,673 + +1.7 2,714,673 + +0.7 2,714,692 + +0.4 2,714,693 - -1.0 2,714,695 + +0.3 2,714,695 + +1.5 2,714,696 - +1.5 2,714,717 - +1.2 2,714,719 - -2.2 2,714,719 - -0.0 2,714,730 + -0.3 2,714,770 + +0.8 2,714,783 - +1.9 2,714,835 + -0.4 2,714,836 - -1.6 2,714,836 - -1.7 2,714,906 + -1.2 2,714,906 + +0.5 2,714,907 - +0.1 2,714,907 - -0.0 2,714,907 - -0.2 2,714,908 + -0.5 2,714,908 + -1.1 2,714,908 + -0.7 2,714,908 + -2.0 2,714,909 - -1.6 2,714,909 - -2.1 2,714,913 + +0.4 2,714,913 + -3.1 2,714,935 + -0.7 2,715,193 + lrhA MCAODC_13870 0.30 -0.2 2,715,210 + lrhA MCAODC_13870 0.32 -2.4 2,715,249 + lrhA MCAODC_13870 0.36 +1.8 2,715,249 + lrhA MCAODC_13870 0.36 -2.2 2,715,250 - lrhA MCAODC_13870 0.36 -2.6 2,715,250 - lrhA MCAODC_13870 0.36 +1.0 2,715,316 + lrhA MCAODC_13870 0.43 -0.2 2,715,316 + lrhA MCAODC_13870 0.43 -0.8 2,715,323 + lrhA MCAODC_13870 0.44 -1.1 2,715,323 + lrhA MCAODC_13870 0.44 -2.2 2,715,323 + lrhA MCAODC_13870 0.44 -0.5 2,715,323 + lrhA MCAODC_13870 0.44 -2.6 2,715,323 + lrhA MCAODC_13870 0.44 +0.2 2,715,323 + lrhA MCAODC_13870 0.44 -0.5 2,715,324 - lrhA MCAODC_13870 0.44 -3.4 2,715,324 - lrhA MCAODC_13870 0.44 +1.7 2,715,324 - lrhA MCAODC_13870 0.44 -1.4 2,715,443 + lrhA MCAODC_13870 0.57 -1.1 2,715,443 + lrhA MCAODC_13870 0.57 +1.7 2,715,444 - lrhA MCAODC_13870 0.57 -1.5 2,715,447 + lrhA MCAODC_13870 0.57 -1.1 2,715,448 - lrhA MCAODC_13870 0.57 -1.5 2,715,448 - lrhA MCAODC_13870 0.57 +0.3 2,715,448 - lrhA MCAODC_13870 0.57 -0.2 2,715,477 + lrhA MCAODC_13870 0.60 +0.6 2,715,477 + lrhA MCAODC_13870 0.60 -2.4 2,715,508 + lrhA MCAODC_13870 0.64 +0.6 2,715,526 + lrhA MCAODC_13870 0.66 -1.6 2,715,527 - lrhA MCAODC_13870 0.66 -0.7 2,715,527 - lrhA MCAODC_13870 0.66 -1.6 2,715,538 + lrhA MCAODC_13870 0.67 -0.7 2,715,538 + lrhA MCAODC_13870 0.67 -2.9 2,715,557 + lrhA MCAODC_13870 0.69 -0.2 2,715,557 + lrhA MCAODC_13870 0.69 +1.3 2,715,557 + lrhA MCAODC_13870 0.69 +1.9 2,715,666 + lrhA MCAODC_13870 0.81 +0.6 2,715,666 + lrhA MCAODC_13870 0.81 +0.3 2,715,675 + lrhA MCAODC_13870 0.82 -3.8 2,715,675 + lrhA MCAODC_13870 0.82 -3.5 2,715,699 - lrhA MCAODC_13870 0.84 +0.7 2,715,710 + lrhA MCAODC_13870 0.85 -1.9 2,715,725 - lrhA MCAODC_13870 0.87 -0.6 2,715,728 - lrhA MCAODC_13870 0.87 -3.5 2,715,728 - lrhA MCAODC_13870 0.87 -0.4 2,715,749 + lrhA MCAODC_13870 0.89 -1.7 2,715,750 - lrhA MCAODC_13870 0.90 -3.3 2,715,786 - -2.6 2,715,795 + -0.4 2,715,796 - +0.4 2,715,859 + -1.9 2,715,863 + -3.8 2,715,864 - +0.7 2,715,878 + +0.4 2,715,910 - -1.0 2,716,017 - -0.9 2,716,042 - +0.3 2,716,086 + MCAODC_13875 0.21 -0.9 2,716,123 - MCAODC_13875 0.44 -3.4 2,716,166 + MCAODC_13875 0.70 -3.1 2,716,167 - MCAODC_13875 0.70 -2.4 2,716,167 - MCAODC_13875 0.70 +0.7 2,716,217 + -0.5 2,716,217 + -3.4 2,716,217 + +3.3 2,716,217 + +0.4 2,716,217 + -0.2 2,716,217 + +0.1 2,716,217 + -1.3 2,716,218 - -0.9 2,716,218 - -0.6 2,716,218 - -2.9 2,716,259 + +1.0 2,716,259 + +0.1 2,716,259 + -2.3 2,716,260 - -1.7 2,716,290 + +0.6 2,716,362 + -2.6 2,716,472 + -4.9 2,716,554 + nuoA MCAODC_13880 0.17 +3.3 2,716,554 + nuoA MCAODC_13880 0.17 -2.8 2,716,554 + nuoA MCAODC_13880 0.17 -2.2 2,716,555 - nuoA MCAODC_13880 0.17 +0.2 2,716,555 - nuoA MCAODC_13880 0.17 -1.4 2,716,663 - nuoA MCAODC_13880 0.42 -4.1 2,716,663 - nuoA MCAODC_13880 0.42 -2.0 2,716,727 + nuoA MCAODC_13880 0.56 -1.2 2,716,727 + nuoA MCAODC_13880 0.56 -0.9 2,716,824 - nuoA MCAODC_13880 0.78 -2.3
Or see this region's nucleotide sequence