Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyA and yphH are separated by 62 nucleotides yphH and yphG are separated by 117 nucleotides
MCAODC_12040: glyA - serine hydroxymethyltransferase, at 2,379,092 to 2,380,345
glyA
MCAODC_12045: yphH - Uncharacterized protein YphH, at 2,380,408 to 2,381,601
yphH
MCAODC_12050: yphG - Uncharacterized protein YphG, at 2,381,719 to 2,385,000
yphG
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2379.678 kb on - strand, within glyA at 2379.987 kb on + strand, within glyA at 2380.111 kb on + strand, within glyA at 2380.221 kb on + strand at 2380.222 kb on - strand at 2380.356 kb on + strand at 2380.393 kb on + strand at 2380.393 kb on + strand at 2380.420 kb on + strand at 2380.421 kb on - strand at 2380.436 kb on + strand at 2380.437 kb on - strand at 2380.437 kb on - strand at 2380.437 kb on - strand at 2380.557 kb on + strand, within yphH at 2380.558 kb on - strand, within yphH at 2380.558 kb on - strand, within yphH at 2380.558 kb on - strand, within yphH at 2380.561 kb on - strand, within yphH at 2380.561 kb on - strand, within yphH at 2380.561 kb on - strand, within yphH at 2380.561 kb on - strand, within yphH at 2380.564 kb on - strand, within yphH at 2380.599 kb on - strand, within yphH at 2380.618 kb on - strand, within yphH at 2380.639 kb on - strand, within yphH at 2380.640 kb on + strand, within yphH at 2380.640 kb on + strand, within yphH at 2380.641 kb on - strand, within yphH at 2380.732 kb on - strand, within yphH at 2380.758 kb on + strand, within yphH at 2380.761 kb on + strand, within yphH at 2380.801 kb on + strand, within yphH at 2380.811 kb on - strand, within yphH at 2380.905 kb on - strand, within yphH at 2381.082 kb on - strand, within yphH at 2381.269 kb on + strand, within yphH at 2381.270 kb on - strand, within yphH at 2381.296 kb on + strand, within yphH at 2381.307 kb on + strand, within yphH at 2381.325 kb on + strand, within yphH at 2381.392 kb on - strand, within yphH at 2381.392 kb on - strand, within yphH at 2381.392 kb on - strand, within yphH at 2381.394 kb on + strand, within yphH at 2381.394 kb on + strand, within yphH at 2381.395 kb on - strand, within yphH at 2381.425 kb on - strand, within yphH at 2381.463 kb on + strand, within yphH at 2381.562 kb on + strand at 2381.563 kb on - strand at 2381.586 kb on + strand at 2381.586 kb on + strand at 2381.666 kb on + strand at 2381.666 kb on + strand at 2381.667 kb on - strand at 2381.703 kb on + strand at 2381.765 kb on - strand at 2381.765 kb on - strand at 2381.765 kb on - strand at 2381.765 kb on - strand at 2381.784 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.786 kb on + strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.787 kb on - strand at 2381.918 kb on - strand at 2381.918 kb on - strand at 2382.069 kb on + strand, within yphG at 2382.070 kb on - strand, within yphG at 2382.070 kb on - strand, within yphG at 2382.102 kb on + strand, within yphG at 2382.103 kb on - strand, within yphG at 2382.185 kb on + strand, within yphG at 2382.344 kb on + strand, within yphG at 2382.344 kb on + strand, within yphG at 2382.345 kb on - strand, within yphG at 2382.406 kb on + strand, within yphG at 2382.406 kb on + strand, within yphG at 2382.406 kb on + strand, within yphG at 2382.407 kb on - strand, within yphG at 2382.407 kb on - strand, within yphG at 2382.407 kb on - strand, within yphG at 2382.409 kb on + strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.410 kb on - strand, within yphG at 2382.421 kb on + strand, within yphG at 2382.422 kb on - strand, within yphG at 2382.475 kb on + strand, within yphG at 2382.475 kb on + strand, within yphG at 2382.475 kb on + strand, within yphG at 2382.475 kb on + strand, within yphG at 2382.475 kb on + strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.476 kb on - strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.477 kb on + strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.478 kb on - strand, within yphG at 2382.542 kb on + strand, within yphG at 2382.542 kb on + strand, within yphG at 2382.543 kb on - strand, within yphG at 2382.561 kb on + strand, within yphG at 2382.561 kb on + strand, within yphG at 2382.562 kb on - strand, within yphG at 2382.574 kb on - strand, within yphG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,379,678 - glyA MCAODC_12040 0.47 -0.9 2,379,987 + glyA MCAODC_12040 0.71 -1.5 2,380,111 + glyA MCAODC_12040 0.81 -2.6 2,380,221 + -0.8 2,380,222 - +5.1 2,380,356 + -1.6 2,380,393 + +0.4 2,380,393 + -2.9 2,380,420 + +0.2 2,380,421 - +0.8 2,380,436 + -1.1 2,380,437 - -0.4 2,380,437 - +0.2 2,380,437 - +1.7 2,380,557 + yphH MCAODC_12045 0.12 +0.6 2,380,558 - yphH MCAODC_12045 0.13 +0.3 2,380,558 - yphH MCAODC_12045 0.13 +0.8 2,380,558 - yphH MCAODC_12045 0.13 +0.7 2,380,561 - yphH MCAODC_12045 0.13 -0.5 2,380,561 - yphH MCAODC_12045 0.13 +2.0 2,380,561 - yphH MCAODC_12045 0.13 +0.5 2,380,561 - yphH MCAODC_12045 0.13 -1.7 2,380,564 - yphH MCAODC_12045 0.13 +0.9 2,380,599 - yphH MCAODC_12045 0.16 +0.9 2,380,618 - yphH MCAODC_12045 0.18 +0.0 2,380,639 - yphH MCAODC_12045 0.19 +1.0 2,380,640 + yphH MCAODC_12045 0.19 +0.1 2,380,640 + yphH MCAODC_12045 0.19 -3.2 2,380,641 - yphH MCAODC_12045 0.20 +1.5 2,380,732 - yphH MCAODC_12045 0.27 -0.8 2,380,758 + yphH MCAODC_12045 0.29 +0.6 2,380,761 + yphH MCAODC_12045 0.30 -0.3 2,380,801 + yphH MCAODC_12045 0.33 -1.7 2,380,811 - yphH MCAODC_12045 0.34 -1.7 2,380,905 - yphH MCAODC_12045 0.42 -2.2 2,381,082 - yphH MCAODC_12045 0.56 +0.5 2,381,269 + yphH MCAODC_12045 0.72 -1.2 2,381,270 - yphH MCAODC_12045 0.72 +0.5 2,381,296 + yphH MCAODC_12045 0.74 -4.6 2,381,307 + yphH MCAODC_12045 0.75 -2.8 2,381,325 + yphH MCAODC_12045 0.77 +2.3 2,381,392 - yphH MCAODC_12045 0.82 -2.1 2,381,392 - yphH MCAODC_12045 0.82 -0.1 2,381,392 - yphH MCAODC_12045 0.82 -0.4 2,381,394 + yphH MCAODC_12045 0.83 -0.5 2,381,394 + yphH MCAODC_12045 0.83 +3.3 2,381,395 - yphH MCAODC_12045 0.83 +1.3 2,381,425 - yphH MCAODC_12045 0.85 +1.7 2,381,463 + yphH MCAODC_12045 0.88 -3.3 2,381,562 + +1.6 2,381,563 - -1.9 2,381,586 + -0.1 2,381,586 + -3.4 2,381,666 + +0.5 2,381,666 + +0.7 2,381,667 - -1.5 2,381,703 + -0.7 2,381,765 - -1.5 2,381,765 - +0.8 2,381,765 - -0.9 2,381,765 - +0.6 2,381,784 + +1.0 2,381,786 + -0.3 2,381,786 + -0.4 2,381,786 + +0.7 2,381,786 + +0.2 2,381,786 + +1.6 2,381,786 + +0.4 2,381,786 + -0.3 2,381,786 + +0.6 2,381,786 + -2.6 2,381,786 + -0.4 2,381,787 - -0.4 2,381,787 - +2.1 2,381,787 - -4.6 2,381,787 - +1.1 2,381,787 - +2.5 2,381,787 - +0.0 2,381,787 - +0.5 2,381,787 - +1.4 2,381,918 - -0.0 2,381,918 - +0.3 2,382,069 + yphG MCAODC_12050 0.11 -3.2 2,382,070 - yphG MCAODC_12050 0.11 -2.6 2,382,070 - yphG MCAODC_12050 0.11 +0.8 2,382,102 + yphG MCAODC_12050 0.12 -1.5 2,382,103 - yphG MCAODC_12050 0.12 -1.1 2,382,185 + yphG MCAODC_12050 0.14 +0.6 2,382,344 + yphG MCAODC_12050 0.19 +0.2 2,382,344 + yphG MCAODC_12050 0.19 -0.1 2,382,345 - yphG MCAODC_12050 0.19 -1.7 2,382,406 + yphG MCAODC_12050 0.21 -0.1 2,382,406 + yphG MCAODC_12050 0.21 -0.6 2,382,406 + yphG MCAODC_12050 0.21 -3.1 2,382,407 - yphG MCAODC_12050 0.21 +0.2 2,382,407 - yphG MCAODC_12050 0.21 +0.9 2,382,407 - yphG MCAODC_12050 0.21 -0.9 2,382,409 + yphG MCAODC_12050 0.21 -2.4 2,382,410 - yphG MCAODC_12050 0.21 +0.1 2,382,410 - yphG MCAODC_12050 0.21 +0.2 2,382,410 - yphG MCAODC_12050 0.21 -0.7 2,382,410 - yphG MCAODC_12050 0.21 +0.3 2,382,410 - yphG MCAODC_12050 0.21 +0.8 2,382,410 - yphG MCAODC_12050 0.21 -1.9 2,382,421 + yphG MCAODC_12050 0.21 -3.3 2,382,422 - yphG MCAODC_12050 0.21 +0.2 2,382,475 + yphG MCAODC_12050 0.23 +1.5 2,382,475 + yphG MCAODC_12050 0.23 +1.4 2,382,475 + yphG MCAODC_12050 0.23 +0.2 2,382,475 + yphG MCAODC_12050 0.23 -0.5 2,382,475 + yphG MCAODC_12050 0.23 -0.4 2,382,476 - yphG MCAODC_12050 0.23 -2.1 2,382,476 - yphG MCAODC_12050 0.23 +0.9 2,382,476 - yphG MCAODC_12050 0.23 -3.1 2,382,476 - yphG MCAODC_12050 0.23 -2.3 2,382,476 - yphG MCAODC_12050 0.23 +0.3 2,382,476 - yphG MCAODC_12050 0.23 +0.6 2,382,476 - yphG MCAODC_12050 0.23 -0.6 2,382,476 - yphG MCAODC_12050 0.23 -2.6 2,382,476 - yphG MCAODC_12050 0.23 -0.6 2,382,477 + yphG MCAODC_12050 0.23 +0.4 2,382,477 + yphG MCAODC_12050 0.23 -1.3 2,382,477 + yphG MCAODC_12050 0.23 -1.6 2,382,477 + yphG MCAODC_12050 0.23 -0.3 2,382,477 + yphG MCAODC_12050 0.23 -0.2 2,382,477 + yphG MCAODC_12050 0.23 -1.2 2,382,478 - yphG MCAODC_12050 0.23 +0.4 2,382,478 - yphG MCAODC_12050 0.23 +1.9 2,382,478 - yphG MCAODC_12050 0.23 +0.6 2,382,478 - yphG MCAODC_12050 0.23 +0.8 2,382,478 - yphG MCAODC_12050 0.23 -0.4 2,382,478 - yphG MCAODC_12050 0.23 +1.6 2,382,478 - yphG MCAODC_12050 0.23 -0.6 2,382,478 - yphG MCAODC_12050 0.23 +0.4 2,382,478 - yphG MCAODC_12050 0.23 +2.2 2,382,478 - yphG MCAODC_12050 0.23 -0.1 2,382,478 - yphG MCAODC_12050 0.23 +0.4 2,382,478 - yphG MCAODC_12050 0.23 -3.2 2,382,478 - yphG MCAODC_12050 0.23 +0.3 2,382,542 + yphG MCAODC_12050 0.25 +0.7 2,382,542 + yphG MCAODC_12050 0.25 -0.1 2,382,543 - yphG MCAODC_12050 0.25 -0.3 2,382,561 + yphG MCAODC_12050 0.26 -0.3 2,382,561 + yphG MCAODC_12050 0.26 +2.0 2,382,562 - yphG MCAODC_12050 0.26 +1.2 2,382,574 - yphG MCAODC_12050 0.26 +0.2
Or see this region's nucleotide sequence