Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt diaA and diaA are separated by 9 nucleotides diaA and yraN are separated by 19 nucleotides yraN and lpoA overlap by 43 nucleotides lpoA and rsmI are separated by 64 nucleotides rsmI and MCAODC_08760 are separated by 42 nucleotides
MCAODC_08735: diaA - DnaA initiator-associating protein DiaA, at 1,746,713 to 1,747,288
diaA
MCAODC_08740: diaA - DnaA initiator-associating protein DiaA, at 1,747,298 to 1,747,888
diaA
MCAODC_08745: yraN - YraN family protein, at 1,747,908 to 1,748,303
yraN
MCAODC_08750: lpoA - penicillin-binding protein activator LpoA, at 1,748,261 to 1,750,297
lpoA
MCAODC_08755: rsmI - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 1,750,362 to 1,751,222
rsmI
MCAODC_08760: MCAODC_08760 - fimbrial protein, at 1,751,265 to 1,751,615
_08760
Position (kb) 1748
1749
1750
1751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1747.290 kb on + strand at 1747.290 kb on + strand at 1747.291 kb on - strand at 1747.299 kb on + strand at 1747.352 kb on + strand at 1747.353 kb on - strand at 1747.363 kb on + strand, within diaA at 1747.364 kb on - strand, within diaA at 1747.428 kb on - strand, within diaA at 1747.489 kb on + strand, within diaA at 1747.512 kb on - strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.553 kb on + strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.554 kb on - strand, within diaA at 1747.593 kb on + strand, within diaA at 1747.594 kb on - strand, within diaA at 1747.594 kb on - strand, within diaA at 1747.608 kb on - strand, within diaA at 1747.608 kb on - strand, within diaA at 1747.608 kb on - strand, within diaA at 1747.608 kb on - strand, within diaA at 1747.648 kb on + strand, within diaA at 1747.648 kb on + strand, within diaA at 1747.649 kb on - strand, within diaA at 1747.652 kb on + strand, within diaA at 1747.653 kb on - strand, within diaA at 1747.653 kb on - strand, within diaA at 1747.653 kb on - strand, within diaA at 1747.668 kb on - strand, within diaA at 1747.874 kb on - strand at 1747.891 kb on + strand at 1747.891 kb on + strand at 1747.899 kb on - strand at 1747.905 kb on + strand at 1747.906 kb on - strand at 1747.906 kb on - strand at 1747.910 kb on - strand at 1747.910 kb on - strand at 1747.929 kb on - strand at 1747.965 kb on + strand, within yraN at 1747.966 kb on - strand, within yraN at 1747.992 kb on - strand, within yraN at 1748.040 kb on + strand, within yraN at 1748.040 kb on + strand, within yraN at 1748.087 kb on - strand, within yraN at 1748.262 kb on + strand at 1748.276 kb on + strand at 1748.276 kb on + strand at 1748.293 kb on - strand at 1748.297 kb on + strand at 1748.298 kb on - strand at 1748.298 kb on - strand at 1748.306 kb on + strand at 1748.307 kb on - strand at 1748.318 kb on - strand at 1748.354 kb on - strand at 1748.357 kb on - strand at 1748.357 kb on - strand at 1748.436 kb on + strand at 1748.436 kb on + strand at 1748.436 kb on + strand at 1748.437 kb on - strand at 1748.437 kb on - strand at 1748.437 kb on - strand at 1748.530 kb on - strand, within lpoA at 1748.647 kb on + strand, within lpoA at 1748.650 kb on + strand, within lpoA at 1748.682 kb on + strand, within lpoA at 1748.682 kb on + strand, within lpoA at 1748.683 kb on - strand, within lpoA at 1748.683 kb on - strand, within lpoA at 1748.683 kb on - strand, within lpoA at 1748.815 kb on + strand, within lpoA at 1748.925 kb on - strand, within lpoA at 1748.989 kb on + strand, within lpoA at 1748.990 kb on - strand, within lpoA at 1749.036 kb on + strand, within lpoA at 1749.037 kb on - strand, within lpoA at 1749.043 kb on + strand, within lpoA at 1749.043 kb on + strand, within lpoA at 1749.083 kb on + strand, within lpoA at 1749.083 kb on + strand, within lpoA at 1749.083 kb on + strand, within lpoA at 1749.083 kb on + strand, within lpoA at 1749.083 kb on + strand, within lpoA at 1749.086 kb on + strand, within lpoA at 1749.086 kb on + strand, within lpoA at 1749.086 kb on + strand, within lpoA at 1749.086 kb on + strand, within lpoA at 1749.087 kb on - strand, within lpoA at 1749.087 kb on - strand, within lpoA at 1749.087 kb on - strand, within lpoA at 1749.087 kb on - strand, within lpoA at 1749.128 kb on - strand, within lpoA at 1749.154 kb on + strand, within lpoA at 1749.154 kb on + strand, within lpoA at 1749.154 kb on + strand, within lpoA at 1749.155 kb on - strand, within lpoA at 1749.183 kb on - strand, within lpoA at 1749.183 kb on - strand, within lpoA at 1749.193 kb on + strand, within lpoA at 1749.194 kb on - strand, within lpoA at 1749.211 kb on + strand, within lpoA at 1749.217 kb on - strand, within lpoA at 1749.284 kb on + strand, within lpoA at 1749.389 kb on + strand, within lpoA at 1749.389 kb on + strand, within lpoA at 1749.389 kb on + strand, within lpoA at 1749.389 kb on + strand, within lpoA at 1749.390 kb on - strand, within lpoA at 1749.390 kb on - strand, within lpoA at 1749.390 kb on - strand, within lpoA at 1749.413 kb on + strand, within lpoA at 1749.485 kb on + strand, within lpoA at 1749.485 kb on + strand, within lpoA at 1749.554 kb on + strand, within lpoA at 1749.554 kb on + strand, within lpoA at 1749.554 kb on + strand, within lpoA at 1749.554 kb on + strand, within lpoA at 1749.555 kb on - strand, within lpoA at 1749.728 kb on - strand, within lpoA at 1749.778 kb on + strand, within lpoA at 1749.778 kb on + strand, within lpoA at 1749.779 kb on - strand, within lpoA at 1749.837 kb on - strand, within lpoA at 1749.837 kb on - strand, within lpoA at 1749.908 kb on + strand, within lpoA at 1749.922 kb on + strand, within lpoA at 1750.031 kb on + strand, within lpoA at 1750.032 kb on - strand, within lpoA at 1750.032 kb on - strand, within lpoA at 1750.032 kb on - strand, within lpoA at 1750.039 kb on + strand, within lpoA at 1750.097 kb on + strand at 1750.097 kb on + strand at 1750.098 kb on - strand at 1750.098 kb on - strand at 1750.117 kb on + strand at 1750.149 kb on + strand at 1750.149 kb on + strand at 1750.183 kb on + strand at 1750.183 kb on + strand at 1750.184 kb on - strand at 1750.184 kb on - strand at 1750.184 kb on - strand at 1750.185 kb on + strand at 1750.185 kb on + strand at 1750.185 kb on + strand at 1750.185 kb on + strand at 1750.185 kb on + strand at 1750.186 kb on - strand at 1750.186 kb on - strand at 1750.186 kb on - strand at 1750.186 kb on - strand at 1750.186 kb on - strand at 1750.186 kb on - strand at 1750.292 kb on + strand at 1750.297 kb on + strand at 1750.297 kb on + strand at 1750.298 kb on - strand at 1750.302 kb on + strand at 1750.302 kb on + strand at 1750.303 kb on - strand at 1750.339 kb on - strand at 1750.339 kb on - strand at 1750.339 kb on - strand at 1750.339 kb on - strand at 1750.411 kb on + strand at 1750.411 kb on + strand at 1750.412 kb on - strand at 1750.462 kb on + strand, within rsmI at 1750.463 kb on - strand, within rsmI at 1750.463 kb on - strand, within rsmI at 1750.514 kb on - strand, within rsmI at 1750.535 kb on + strand, within rsmI at 1750.536 kb on - strand, within rsmI at 1750.655 kb on + strand, within rsmI at 1750.682 kb on + strand, within rsmI at 1750.762 kb on + strand, within rsmI at 1750.782 kb on + strand, within rsmI at 1750.874 kb on + strand, within rsmI at 1750.875 kb on - strand, within rsmI at 1750.888 kb on + strand, within rsmI at 1750.889 kb on - strand, within rsmI at 1750.905 kb on - strand, within rsmI at 1750.905 kb on - strand, within rsmI at 1750.918 kb on + strand, within rsmI at 1751.066 kb on + strand, within rsmI at 1751.067 kb on - strand, within rsmI at 1751.067 kb on - strand, within rsmI at 1751.067 kb on - strand, within rsmI at 1751.067 kb on - strand, within rsmI at 1751.193 kb on + strand at 1751.194 kb on - strand at 1751.194 kb on - strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.195 kb on + strand at 1751.196 kb on - strand at 1751.196 kb on - strand at 1751.196 kb on - strand at 1751.196 kb on - strand at 1751.196 kb on - strand at 1751.199 kb on + strand at 1751.199 kb on + strand at 1751.199 kb on + strand at 1751.199 kb on + strand at 1751.199 kb on + strand at 1751.200 kb on - strand at 1751.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,747,290 + +0.2 1,747,290 + -1.6 1,747,291 - +0.2 1,747,299 + -2.2 1,747,352 + +2.1 1,747,353 - +1.1 1,747,363 + diaA MCAODC_08740 0.11 +0.6 1,747,364 - diaA MCAODC_08740 0.11 -2.4 1,747,428 - diaA MCAODC_08740 0.22 -2.8 1,747,489 + diaA MCAODC_08740 0.32 +0.0 1,747,512 - diaA MCAODC_08740 0.36 -0.7 1,747,553 + diaA MCAODC_08740 0.43 -4.1 1,747,553 + diaA MCAODC_08740 0.43 +1.1 1,747,553 + diaA MCAODC_08740 0.43 +0.5 1,747,553 + diaA MCAODC_08740 0.43 +0.5 1,747,553 + diaA MCAODC_08740 0.43 -0.2 1,747,553 + diaA MCAODC_08740 0.43 +0.8 1,747,553 + diaA MCAODC_08740 0.43 -2.3 1,747,554 - diaA MCAODC_08740 0.43 +1.2 1,747,554 - diaA MCAODC_08740 0.43 -1.0 1,747,554 - diaA MCAODC_08740 0.43 -2.8 1,747,554 - diaA MCAODC_08740 0.43 -2.2 1,747,554 - diaA MCAODC_08740 0.43 -0.4 1,747,554 - diaA MCAODC_08740 0.43 -1.7 1,747,593 + diaA MCAODC_08740 0.50 +0.8 1,747,594 - diaA MCAODC_08740 0.50 +0.2 1,747,594 - diaA MCAODC_08740 0.50 +0.1 1,747,608 - diaA MCAODC_08740 0.52 +1.5 1,747,608 - diaA MCAODC_08740 0.52 -0.7 1,747,608 - diaA MCAODC_08740 0.52 -2.9 1,747,608 - diaA MCAODC_08740 0.52 -1.2 1,747,648 + diaA MCAODC_08740 0.59 +0.1 1,747,648 + diaA MCAODC_08740 0.59 -2.3 1,747,649 - diaA MCAODC_08740 0.59 +0.1 1,747,652 + diaA MCAODC_08740 0.60 +0.7 1,747,653 - diaA MCAODC_08740 0.60 +1.1 1,747,653 - diaA MCAODC_08740 0.60 +1.1 1,747,653 - diaA MCAODC_08740 0.60 -0.3 1,747,668 - diaA MCAODC_08740 0.63 -3.0 1,747,874 - -0.3 1,747,891 + -2.3 1,747,891 + +0.4 1,747,899 - +0.6 1,747,905 + +1.9 1,747,906 - +0.1 1,747,906 - -0.9 1,747,910 - -0.1 1,747,910 - +0.1 1,747,929 - -0.7 1,747,965 + yraN MCAODC_08745 0.14 +1.1 1,747,966 - yraN MCAODC_08745 0.15 -0.4 1,747,992 - yraN MCAODC_08745 0.21 +0.1 1,748,040 + yraN MCAODC_08745 0.33 -2.1 1,748,040 + yraN MCAODC_08745 0.33 +0.6 1,748,087 - yraN MCAODC_08745 0.45 +0.1 1,748,262 + +0.8 1,748,276 + -0.3 1,748,276 + -0.4 1,748,293 - +1.7 1,748,297 + -3.8 1,748,298 - +0.8 1,748,298 - +0.9 1,748,306 + -3.5 1,748,307 - -2.0 1,748,318 - -0.6 1,748,354 - -4.5 1,748,357 - -3.1 1,748,357 - -0.4 1,748,436 + +2.2 1,748,436 + -0.5 1,748,436 + +0.5 1,748,437 - -0.8 1,748,437 - +1.4 1,748,437 - -4.4 1,748,530 - lpoA MCAODC_08750 0.13 +0.8 1,748,647 + lpoA MCAODC_08750 0.19 +0.2 1,748,650 + lpoA MCAODC_08750 0.19 -0.9 1,748,682 + lpoA MCAODC_08750 0.21 +0.3 1,748,682 + lpoA MCAODC_08750 0.21 +1.0 1,748,683 - lpoA MCAODC_08750 0.21 -0.9 1,748,683 - lpoA MCAODC_08750 0.21 -0.1 1,748,683 - lpoA MCAODC_08750 0.21 -0.5 1,748,815 + lpoA MCAODC_08750 0.27 -1.6 1,748,925 - lpoA MCAODC_08750 0.33 +0.4 1,748,989 + lpoA MCAODC_08750 0.36 -2.5 1,748,990 - lpoA MCAODC_08750 0.36 +0.3 1,749,036 + lpoA MCAODC_08750 0.38 +1.2 1,749,037 - lpoA MCAODC_08750 0.38 +0.3 1,749,043 + lpoA MCAODC_08750 0.38 -0.2 1,749,043 + lpoA MCAODC_08750 0.38 -0.4 1,749,083 + lpoA MCAODC_08750 0.40 +1.1 1,749,083 + lpoA MCAODC_08750 0.40 -0.6 1,749,083 + lpoA MCAODC_08750 0.40 -0.4 1,749,083 + lpoA MCAODC_08750 0.40 +1.4 1,749,083 + lpoA MCAODC_08750 0.40 -2.8 1,749,086 + lpoA MCAODC_08750 0.41 +1.5 1,749,086 + lpoA MCAODC_08750 0.41 +1.4 1,749,086 + lpoA MCAODC_08750 0.41 +0.4 1,749,086 + lpoA MCAODC_08750 0.41 -2.1 1,749,087 - lpoA MCAODC_08750 0.41 -1.1 1,749,087 - lpoA MCAODC_08750 0.41 +0.4 1,749,087 - lpoA MCAODC_08750 0.41 -0.6 1,749,087 - lpoA MCAODC_08750 0.41 -0.4 1,749,128 - lpoA MCAODC_08750 0.43 -3.3 1,749,154 + lpoA MCAODC_08750 0.44 +3.1 1,749,154 + lpoA MCAODC_08750 0.44 -3.3 1,749,154 + lpoA MCAODC_08750 0.44 -1.6 1,749,155 - lpoA MCAODC_08750 0.44 -3.7 1,749,183 - lpoA MCAODC_08750 0.45 -0.1 1,749,183 - lpoA MCAODC_08750 0.45 +0.0 1,749,193 + lpoA MCAODC_08750 0.46 -4.5 1,749,194 - lpoA MCAODC_08750 0.46 +2.2 1,749,211 + lpoA MCAODC_08750 0.47 +0.3 1,749,217 - lpoA MCAODC_08750 0.47 -2.7 1,749,284 + lpoA MCAODC_08750 0.50 +0.2 1,749,389 + lpoA MCAODC_08750 0.55 +1.6 1,749,389 + lpoA MCAODC_08750 0.55 +0.4 1,749,389 + lpoA MCAODC_08750 0.55 -2.6 1,749,389 + lpoA MCAODC_08750 0.55 +1.3 1,749,390 - lpoA MCAODC_08750 0.55 -2.8 1,749,390 - lpoA MCAODC_08750 0.55 -1.2 1,749,390 - lpoA MCAODC_08750 0.55 -0.4 1,749,413 + lpoA MCAODC_08750 0.57 -2.8 1,749,485 + lpoA MCAODC_08750 0.60 +0.3 1,749,485 + lpoA MCAODC_08750 0.60 +0.3 1,749,554 + lpoA MCAODC_08750 0.63 -0.4 1,749,554 + lpoA MCAODC_08750 0.63 -2.8 1,749,554 + lpoA MCAODC_08750 0.63 +1.1 1,749,554 + lpoA MCAODC_08750 0.63 +0.1 1,749,555 - lpoA MCAODC_08750 0.64 -0.1 1,749,728 - lpoA MCAODC_08750 0.72 -2.4 1,749,778 + lpoA MCAODC_08750 0.74 +0.2 1,749,778 + lpoA MCAODC_08750 0.74 +0.4 1,749,779 - lpoA MCAODC_08750 0.75 +1.4 1,749,837 - lpoA MCAODC_08750 0.77 -1.2 1,749,837 - lpoA MCAODC_08750 0.77 +0.7 1,749,908 + lpoA MCAODC_08750 0.81 -0.8 1,749,922 + lpoA MCAODC_08750 0.82 -0.4 1,750,031 + lpoA MCAODC_08750 0.87 +0.3 1,750,032 - lpoA MCAODC_08750 0.87 -3.1 1,750,032 - lpoA MCAODC_08750 0.87 -0.4 1,750,032 - lpoA MCAODC_08750 0.87 -2.0 1,750,039 + lpoA MCAODC_08750 0.87 -3.2 1,750,097 + +0.5 1,750,097 + +0.4 1,750,098 - +0.5 1,750,098 - -1.1 1,750,117 + +0.7 1,750,149 + -0.1 1,750,149 + -3.7 1,750,183 + +0.8 1,750,183 + -3.0 1,750,184 - +0.2 1,750,184 - +0.6 1,750,184 - +0.3 1,750,185 + -0.2 1,750,185 + -4.4 1,750,185 + +1.3 1,750,185 + +0.1 1,750,185 + +0.0 1,750,186 - -2.1 1,750,186 - -2.1 1,750,186 - -0.2 1,750,186 - +1.7 1,750,186 - +0.4 1,750,186 - +0.1 1,750,292 + -1.2 1,750,297 + -1.7 1,750,297 + -2.2 1,750,298 - -2.5 1,750,302 + -3.3 1,750,302 + -2.0 1,750,303 - -3.3 1,750,339 - +1.1 1,750,339 - -0.1 1,750,339 - -2.7 1,750,339 - +1.0 1,750,411 + -3.3 1,750,411 + -2.5 1,750,412 - -1.9 1,750,462 + rsmI MCAODC_08755 0.12 +0.2 1,750,463 - rsmI MCAODC_08755 0.12 +1.2 1,750,463 - rsmI MCAODC_08755 0.12 +0.9 1,750,514 - rsmI MCAODC_08755 0.18 -3.4 1,750,535 + rsmI MCAODC_08755 0.20 -0.2 1,750,536 - rsmI MCAODC_08755 0.20 +1.2 1,750,655 + rsmI MCAODC_08755 0.34 -0.2 1,750,682 + rsmI MCAODC_08755 0.37 +1.6 1,750,762 + rsmI MCAODC_08755 0.46 -1.2 1,750,782 + rsmI MCAODC_08755 0.49 -3.0 1,750,874 + rsmI MCAODC_08755 0.59 +0.2 1,750,875 - rsmI MCAODC_08755 0.60 +0.4 1,750,888 + rsmI MCAODC_08755 0.61 +0.1 1,750,889 - rsmI MCAODC_08755 0.61 +1.7 1,750,905 - rsmI MCAODC_08755 0.63 -1.2 1,750,905 - rsmI MCAODC_08755 0.63 -0.2 1,750,918 + rsmI MCAODC_08755 0.65 -0.5 1,751,066 + rsmI MCAODC_08755 0.82 +0.3 1,751,067 - rsmI MCAODC_08755 0.82 -3.1 1,751,067 - rsmI MCAODC_08755 0.82 +0.9 1,751,067 - rsmI MCAODC_08755 0.82 -2.5 1,751,067 - rsmI MCAODC_08755 0.82 +1.4 1,751,193 + -2.3 1,751,194 - -3.8 1,751,194 - -0.7 1,751,195 + -2.2 1,751,195 + -0.6 1,751,195 + -0.4 1,751,195 + -1.9 1,751,195 + +2.4 1,751,195 + -0.0 1,751,195 + +0.1 1,751,195 + +1.7 1,751,196 - -1.7 1,751,196 - +1.6 1,751,196 - -0.2 1,751,196 - -1.2 1,751,196 - +0.6 1,751,199 + +0.6 1,751,199 + +0.4 1,751,199 + -2.9 1,751,199 + +0.5 1,751,199 + -2.2 1,751,200 - -1.5 1,751,221 - +1.4
Or see this region's nucleotide sequence