Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 164 nucleotides rbfA and truB overlap by 1 nucleotides truB and rpsO are separated by 148 nucleotides rpsO and pnp are separated by 246 nucleotides
MCAODC_08630: infB - Translation initiation factor IF-2, at 1,727,683 to 1,730,355
infB
MCAODC_08640: rbfA - 30S ribosome-binding factor RbfA, at 1,730,520 to 1,730,921
rbfA
MCAODC_08645: truB - tRNA pseudouridine(55) synthase TruB, at 1,730,921 to 1,731,865
truB
MCAODC_08650: rpsO - 30S ribosomal protein S15, at 1,732,014 to 1,732,283
rpsO
MCAODC_08660: pnp - polyribonucleotide nucleotidyltransferase, at 1,732,530 to 1,734,665
pnp
Position (kb)
1730
1731
1732 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1730.318 kb on + strand at 1730.343 kb on + strand at 1730.343 kb on + strand at 1730.362 kb on + strand at 1730.434 kb on + strand at 1730.488 kb on + strand at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.843 kb on + strand, within rbfA at 1730.919 kb on + strand at 1730.975 kb on - strand at 1730.986 kb on + strand at 1730.986 kb on + strand at 1730.986 kb on + strand at 1730.987 kb on - strand at 1730.987 kb on - strand at 1731.022 kb on + strand, within truB at 1731.022 kb on + strand, within truB at 1731.022 kb on + strand, within truB at 1731.022 kb on + strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.023 kb on - strand, within truB at 1731.024 kb on + strand, within truB at 1731.024 kb on + strand, within truB at 1731.024 kb on + strand, within truB at 1731.024 kb on + strand, within truB at 1731.025 kb on - strand, within truB at 1731.025 kb on - strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.026 kb on + strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.027 kb on - strand, within truB at 1731.028 kb on + strand, within truB at 1731.028 kb on + strand, within truB at 1731.029 kb on - strand, within truB at 1731.029 kb on - strand, within truB at 1731.029 kb on - strand, within truB at 1731.050 kb on - strand, within truB at 1731.182 kb on - strand, within truB at 1731.229 kb on + strand, within truB at 1731.296 kb on + strand, within truB at 1731.296 kb on + strand, within truB at 1731.297 kb on - strand, within truB at 1731.311 kb on + strand, within truB at 1731.312 kb on - strand, within truB at 1731.330 kb on - strand, within truB at 1731.335 kb on + strand, within truB at 1731.336 kb on - strand, within truB at 1731.401 kb on - strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.455 kb on + strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.456 kb on - strand, within truB at 1731.457 kb on + strand, within truB at 1731.458 kb on - strand, within truB at 1731.458 kb on - strand, within truB at 1731.515 kb on + strand, within truB at 1731.516 kb on - strand, within truB at 1731.535 kb on - strand, within truB at 1731.538 kb on + strand, within truB at 1731.539 kb on - strand, within truB at 1731.539 kb on - strand, within truB at 1731.539 kb on - strand, within truB at 1731.543 kb on - strand, within truB at 1731.543 kb on - strand, within truB at 1731.611 kb on - strand, within truB at 1731.640 kb on - strand, within truB at 1731.640 kb on - strand, within truB at 1731.707 kb on + strand, within truB at 1731.708 kb on - strand, within truB at 1731.734 kb on - strand, within truB at 1731.799 kb on + strand at 1731.970 kb on + strand at 1732.357 kb on + strand at 1732.358 kb on - strand at 1732.362 kb on + strand at 1732.362 kb on + strand at 1732.363 kb on - strand at 1732.363 kb on - strand at 1732.363 kb on - strand at 1732.372 kb on - strand at 1732.428 kb on - strand at 1732.450 kb on + strand at 1732.482 kb on - strand at 1732.501 kb on - strand at 1732.526 kb on + strand at 1732.551 kb on - strand at 1732.712 kb on + strand at 1732.728 kb on - strand at 1732.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,730,318 + -0.9 1,730,343 + +0.8 1,730,343 + -2.4 1,730,362 + +0.7 1,730,434 + -0.8 1,730,488 + -1.4 1,730,843 + rbfA MCAODC_08640 0.80 -3.5 1,730,843 + rbfA MCAODC_08640 0.80 -1.7 1,730,843 + rbfA MCAODC_08640 0.80 -1.4 1,730,843 + rbfA MCAODC_08640 0.80 -0.9 1,730,843 + rbfA MCAODC_08640 0.80 +0.9 1,730,843 + rbfA MCAODC_08640 0.80 -1.9 1,730,843 + rbfA MCAODC_08640 0.80 +0.5 1,730,919 + -2.5 1,730,975 - -3.2 1,730,986 + +1.0 1,730,986 + -2.5 1,730,986 + -0.4 1,730,987 - -1.0 1,730,987 - -1.6 1,731,022 + truB MCAODC_08645 0.11 +0.2 1,731,022 + truB MCAODC_08645 0.11 -1.6 1,731,022 + truB MCAODC_08645 0.11 -0.2 1,731,022 + truB MCAODC_08645 0.11 -1.2 1,731,023 - truB MCAODC_08645 0.11 -0.5 1,731,023 - truB MCAODC_08645 0.11 -0.0 1,731,023 - truB MCAODC_08645 0.11 -1.3 1,731,023 - truB MCAODC_08645 0.11 -1.2 1,731,023 - truB MCAODC_08645 0.11 +0.6 1,731,023 - truB MCAODC_08645 0.11 +0.8 1,731,023 - truB MCAODC_08645 0.11 +1.7 1,731,024 + truB MCAODC_08645 0.11 +1.7 1,731,024 + truB MCAODC_08645 0.11 -0.1 1,731,024 + truB MCAODC_08645 0.11 -2.6 1,731,024 + truB MCAODC_08645 0.11 -0.1 1,731,025 - truB MCAODC_08645 0.11 +1.2 1,731,025 - truB MCAODC_08645 0.11 +0.4 1,731,026 + truB MCAODC_08645 0.11 -0.2 1,731,026 + truB MCAODC_08645 0.11 -0.6 1,731,026 + truB MCAODC_08645 0.11 +1.1 1,731,026 + truB MCAODC_08645 0.11 +1.0 1,731,026 + truB MCAODC_08645 0.11 +0.2 1,731,026 + truB MCAODC_08645 0.11 -3.8 1,731,026 + truB MCAODC_08645 0.11 +0.1 1,731,027 - truB MCAODC_08645 0.11 +1.4 1,731,027 - truB MCAODC_08645 0.11 +1.9 1,731,027 - truB MCAODC_08645 0.11 +0.6 1,731,027 - truB MCAODC_08645 0.11 +2.2 1,731,027 - truB MCAODC_08645 0.11 -3.6 1,731,027 - truB MCAODC_08645 0.11 -0.9 1,731,027 - truB MCAODC_08645 0.11 +1.2 1,731,027 - truB MCAODC_08645 0.11 +2.1 1,731,027 - truB MCAODC_08645 0.11 -1.1 1,731,027 - truB MCAODC_08645 0.11 -0.8 1,731,027 - truB MCAODC_08645 0.11 +2.0 1,731,028 + truB MCAODC_08645 0.11 +0.5 1,731,028 + truB MCAODC_08645 0.11 +0.4 1,731,029 - truB MCAODC_08645 0.11 -0.6 1,731,029 - truB MCAODC_08645 0.11 -0.4 1,731,029 - truB MCAODC_08645 0.11 -0.1 1,731,050 - truB MCAODC_08645 0.14 +0.6 1,731,182 - truB MCAODC_08645 0.28 +1.5 1,731,229 + truB MCAODC_08645 0.33 -0.8 1,731,296 + truB MCAODC_08645 0.40 -0.7 1,731,296 + truB MCAODC_08645 0.40 +0.2 1,731,297 - truB MCAODC_08645 0.40 +0.5 1,731,311 + truB MCAODC_08645 0.41 -2.2 1,731,312 - truB MCAODC_08645 0.41 -0.2 1,731,330 - truB MCAODC_08645 0.43 -0.3 1,731,335 + truB MCAODC_08645 0.44 -0.2 1,731,336 - truB MCAODC_08645 0.44 -0.5 1,731,401 - truB MCAODC_08645 0.51 -1.5 1,731,455 + truB MCAODC_08645 0.57 +0.1 1,731,455 + truB MCAODC_08645 0.57 -0.9 1,731,455 + truB MCAODC_08645 0.57 -2.2 1,731,455 + truB MCAODC_08645 0.57 -0.9 1,731,455 + truB MCAODC_08645 0.57 +0.6 1,731,455 + truB MCAODC_08645 0.57 -4.6 1,731,455 + truB MCAODC_08645 0.57 +0.8 1,731,455 + truB MCAODC_08645 0.57 -0.1 1,731,455 + truB MCAODC_08645 0.57 +0.3 1,731,456 - truB MCAODC_08645 0.57 +1.2 1,731,456 - truB MCAODC_08645 0.57 +0.4 1,731,456 - truB MCAODC_08645 0.57 -2.0 1,731,456 - truB MCAODC_08645 0.57 -3.0 1,731,456 - truB MCAODC_08645 0.57 -1.4 1,731,456 - truB MCAODC_08645 0.57 +1.6 1,731,456 - truB MCAODC_08645 0.57 -0.0 1,731,456 - truB MCAODC_08645 0.57 +0.7 1,731,456 - truB MCAODC_08645 0.57 +0.5 1,731,456 - truB MCAODC_08645 0.57 -0.2 1,731,456 - truB MCAODC_08645 0.57 -0.7 1,731,457 + truB MCAODC_08645 0.57 -2.8 1,731,458 - truB MCAODC_08645 0.57 -0.2 1,731,458 - truB MCAODC_08645 0.57 +2.2 1,731,515 + truB MCAODC_08645 0.63 -0.7 1,731,516 - truB MCAODC_08645 0.63 -0.5 1,731,535 - truB MCAODC_08645 0.65 +1.4 1,731,538 + truB MCAODC_08645 0.65 -1.6 1,731,539 - truB MCAODC_08645 0.65 +0.9 1,731,539 - truB MCAODC_08645 0.65 +0.4 1,731,539 - truB MCAODC_08645 0.65 +0.4 1,731,543 - truB MCAODC_08645 0.66 +1.4 1,731,543 - truB MCAODC_08645 0.66 +0.7 1,731,611 - truB MCAODC_08645 0.73 +1.7 1,731,640 - truB MCAODC_08645 0.76 -3.0 1,731,640 - truB MCAODC_08645 0.76 -2.2 1,731,707 + truB MCAODC_08645 0.83 +0.2 1,731,708 - truB MCAODC_08645 0.83 -1.2 1,731,734 - truB MCAODC_08645 0.86 +1.0 1,731,799 + +0.6 1,731,970 + -0.8 1,732,357 + -1.0 1,732,358 - +0.3 1,732,362 + -5.1 1,732,362 + -2.8 1,732,363 - -1.3 1,732,363 - -1.5 1,732,363 - +1.1 1,732,372 - -2.2 1,732,428 - +0.3 1,732,450 + -2.3 1,732,482 - -2.2 1,732,501 - -0.2 1,732,526 + -2.2 1,732,551 - -2.3 1,732,712 + -2.5 1,732,728 - -1.6 1,732,732 - -0.4
Or see this region's nucleotide sequence