Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ghrB and yiaD are separated by 103 nucleotides yiaD and bisC are separated by 152 nucleotides
MCAODC_06535: ghrB - glyoxylate/hydroxypyruvate reductase GhrB, at 1,320,312 to 1,321,286
ghrB
MCAODC_06540: yiaD - OmpA family lipoprotein, at 1,321,390 to 1,322,049
yiaD
MCAODC_06545: bisC - biotin sulfoxide reductase, at 1,322,202 to 1,324,535
bisC
Position (kb)
1321
1322
1323 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1320.432 kb on - strand, within ghrB at 1320.439 kb on - strand, within ghrB at 1320.658 kb on - strand, within ghrB at 1320.693 kb on + strand, within ghrB at 1320.693 kb on + strand, within ghrB at 1320.694 kb on - strand, within ghrB at 1320.694 kb on - strand, within ghrB at 1320.694 kb on - strand, within ghrB at 1320.702 kb on - strand, within ghrB at 1320.746 kb on + strand, within ghrB at 1320.764 kb on + strand, within ghrB at 1320.766 kb on + strand, within ghrB at 1320.766 kb on + strand, within ghrB at 1320.767 kb on - strand, within ghrB at 1320.885 kb on + strand, within ghrB at 1320.885 kb on + strand, within ghrB at 1320.885 kb on + strand, within ghrB at 1320.913 kb on - strand, within ghrB at 1320.924 kb on + strand, within ghrB at 1320.991 kb on - strand, within ghrB at 1321.057 kb on + strand, within ghrB at 1321.058 kb on - strand, within ghrB at 1321.242 kb on + strand at 1321.242 kb on + strand at 1321.242 kb on + strand at 1321.243 kb on - strand at 1321.243 kb on - strand at 1321.254 kb on + strand at 1321.264 kb on + strand at 1321.306 kb on + strand at 1321.307 kb on - strand at 1321.320 kb on + strand at 1321.330 kb on + strand at 1321.331 kb on - strand at 1321.331 kb on - strand at 1321.331 kb on - strand at 1321.391 kb on + strand at 1321.407 kb on + strand at 1321.408 kb on - strand at 1321.597 kb on - strand, within yiaD at 1321.598 kb on + strand, within yiaD at 1321.599 kb on - strand, within yiaD at 1321.599 kb on - strand, within yiaD at 1321.599 kb on - strand, within yiaD at 1321.599 kb on - strand, within yiaD at 1321.722 kb on + strand, within yiaD at 1321.732 kb on - strand, within yiaD at 1321.753 kb on + strand, within yiaD at 1321.758 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.811 kb on + strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.812 kb on - strand, within yiaD at 1321.815 kb on - strand, within yiaD at 1321.815 kb on - strand, within yiaD at 1321.974 kb on - strand, within yiaD at 1321.974 kb on - strand, within yiaD at 1321.974 kb on - strand, within yiaD at 1321.980 kb on + strand, within yiaD at 1321.980 kb on + strand, within yiaD at 1321.981 kb on - strand, within yiaD at 1321.996 kb on + strand at 1322.029 kb on - strand at 1322.060 kb on + strand at 1322.060 kb on + strand at 1322.060 kb on + strand at 1322.061 kb on - strand at 1322.061 kb on - strand at 1322.063 kb on + strand at 1322.104 kb on + strand at 1322.104 kb on + strand at 1322.126 kb on + strand at 1322.126 kb on + strand at 1322.127 kb on - strand at 1322.127 kb on - strand at 1322.156 kb on + strand at 1322.156 kb on + strand at 1322.160 kb on - strand at 1322.164 kb on - strand at 1322.164 kb on - strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.174 kb on + strand at 1322.175 kb on - strand at 1322.175 kb on - strand at 1322.175 kb on - strand at 1322.180 kb on + strand at 1322.180 kb on + strand at 1322.180 kb on + strand at 1322.181 kb on - strand at 1322.289 kb on - strand at 1322.364 kb on - strand at 1322.364 kb on - strand at 1322.481 kb on - strand, within bisC at 1322.481 kb on - strand, within bisC at 1322.495 kb on + strand, within bisC at 1322.496 kb on - strand, within bisC at 1322.514 kb on + strand, within bisC at 1322.514 kb on + strand, within bisC at 1322.515 kb on - strand, within bisC at 1322.515 kb on - strand, within bisC at 1322.544 kb on + strand, within bisC at 1322.574 kb on - strand, within bisC at 1322.574 kb on - strand, within bisC at 1322.588 kb on - strand, within bisC at 1322.588 kb on - strand, within bisC at 1322.588 kb on - strand, within bisC at 1322.590 kb on + strand, within bisC at 1322.590 kb on + strand, within bisC at 1322.591 kb on - strand, within bisC at 1322.591 kb on - strand, within bisC at 1322.591 kb on - strand, within bisC at 1322.598 kb on + strand, within bisC at 1322.598 kb on + strand, within bisC at 1322.598 kb on + strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.599 kb on - strand, within bisC at 1322.600 kb on + strand, within bisC at 1322.601 kb on - strand, within bisC at 1322.621 kb on + strand, within bisC at 1322.621 kb on + strand, within bisC at 1322.621 kb on + strand, within bisC at 1322.641 kb on - strand, within bisC at 1322.673 kb on + strand, within bisC at 1322.673 kb on + strand, within bisC at 1322.674 kb on - strand, within bisC at 1322.688 kb on - strand, within bisC at 1322.688 kb on - strand, within bisC at 1322.697 kb on + strand, within bisC at 1322.698 kb on - strand, within bisC at 1322.735 kb on + strand, within bisC at 1322.762 kb on + strand, within bisC at 1323.025 kb on - strand, within bisC at 1323.042 kb on + strand, within bisC at 1323.042 kb on + strand, within bisC at 1323.043 kb on - strand, within bisC at 1323.043 kb on - strand, within bisC at 1323.043 kb on - strand, within bisC at 1323.043 kb on - strand, within bisC at 1323.043 kb on - strand, within bisC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,320,432 - ghrB MCAODC_06535 0.12 -2.0 1,320,439 - ghrB MCAODC_06535 0.13 +1.4 1,320,658 - ghrB MCAODC_06535 0.35 +0.9 1,320,693 + ghrB MCAODC_06535 0.39 -0.4 1,320,693 + ghrB MCAODC_06535 0.39 -1.0 1,320,694 - ghrB MCAODC_06535 0.39 +0.5 1,320,694 - ghrB MCAODC_06535 0.39 +0.3 1,320,694 - ghrB MCAODC_06535 0.39 -2.0 1,320,702 - ghrB MCAODC_06535 0.40 -3.4 1,320,746 + ghrB MCAODC_06535 0.45 -0.2 1,320,764 + ghrB MCAODC_06535 0.46 -1.1 1,320,766 + ghrB MCAODC_06535 0.47 +0.4 1,320,766 + ghrB MCAODC_06535 0.47 +0.4 1,320,767 - ghrB MCAODC_06535 0.47 +0.4 1,320,885 + ghrB MCAODC_06535 0.59 -0.1 1,320,885 + ghrB MCAODC_06535 0.59 -0.1 1,320,885 + ghrB MCAODC_06535 0.59 -0.4 1,320,913 - ghrB MCAODC_06535 0.62 +0.7 1,320,924 + ghrB MCAODC_06535 0.63 +0.8 1,320,991 - ghrB MCAODC_06535 0.70 -3.5 1,321,057 + ghrB MCAODC_06535 0.76 -1.5 1,321,058 - ghrB MCAODC_06535 0.77 +0.0 1,321,242 + -4.1 1,321,242 + -3.0 1,321,242 + -1.6 1,321,243 - -1.3 1,321,243 - +0.4 1,321,254 + -0.2 1,321,264 + +2.3 1,321,306 + -0.5 1,321,307 - +0.4 1,321,320 + -1.0 1,321,330 + +3.2 1,321,331 - -0.6 1,321,331 - +2.9 1,321,331 - -2.1 1,321,391 + -1.6 1,321,407 + +0.2 1,321,408 - -0.1 1,321,597 - yiaD MCAODC_06540 0.31 -0.1 1,321,598 + yiaD MCAODC_06540 0.32 -2.5 1,321,599 - yiaD MCAODC_06540 0.32 +1.1 1,321,599 - yiaD MCAODC_06540 0.32 +2.0 1,321,599 - yiaD MCAODC_06540 0.32 +1.8 1,321,599 - yiaD MCAODC_06540 0.32 -3.3 1,321,722 + yiaD MCAODC_06540 0.50 +0.3 1,321,732 - yiaD MCAODC_06540 0.52 +0.4 1,321,753 + yiaD MCAODC_06540 0.55 -0.3 1,321,758 + yiaD MCAODC_06540 0.56 +0.9 1,321,811 + yiaD MCAODC_06540 0.64 -1.5 1,321,811 + yiaD MCAODC_06540 0.64 +0.8 1,321,811 + yiaD MCAODC_06540 0.64 -0.1 1,321,811 + yiaD MCAODC_06540 0.64 -0.1 1,321,811 + yiaD MCAODC_06540 0.64 +0.2 1,321,811 + yiaD MCAODC_06540 0.64 +0.9 1,321,811 + yiaD MCAODC_06540 0.64 -3.2 1,321,811 + yiaD MCAODC_06540 0.64 +1.9 1,321,811 + yiaD MCAODC_06540 0.64 +0.2 1,321,812 - yiaD MCAODC_06540 0.64 +0.6 1,321,812 - yiaD MCAODC_06540 0.64 -0.1 1,321,812 - yiaD MCAODC_06540 0.64 +2.5 1,321,812 - yiaD MCAODC_06540 0.64 +0.8 1,321,812 - yiaD MCAODC_06540 0.64 -2.9 1,321,812 - yiaD MCAODC_06540 0.64 +0.8 1,321,812 - yiaD MCAODC_06540 0.64 -0.0 1,321,815 - yiaD MCAODC_06540 0.64 -0.4 1,321,815 - yiaD MCAODC_06540 0.64 -1.0 1,321,974 - yiaD MCAODC_06540 0.88 +0.0 1,321,974 - yiaD MCAODC_06540 0.88 +0.0 1,321,974 - yiaD MCAODC_06540 0.88 +0.1 1,321,980 + yiaD MCAODC_06540 0.89 +0.1 1,321,980 + yiaD MCAODC_06540 0.89 -0.7 1,321,981 - yiaD MCAODC_06540 0.90 -1.9 1,321,996 + +0.3 1,322,029 - -0.2 1,322,060 + -1.3 1,322,060 + -0.3 1,322,060 + +1.1 1,322,061 - +1.3 1,322,061 - +0.1 1,322,063 + -2.9 1,322,104 + +0.1 1,322,104 + -0.1 1,322,126 + +2.8 1,322,126 + -2.4 1,322,127 - +2.0 1,322,127 - +0.0 1,322,156 + -2.7 1,322,156 + -0.7 1,322,160 - +0.5 1,322,164 - +1.1 1,322,164 - -1.1 1,322,174 + +1.3 1,322,174 + +0.1 1,322,174 + -0.2 1,322,174 + +0.6 1,322,174 + +0.7 1,322,174 + -0.2 1,322,174 + -0.2 1,322,174 + -0.4 1,322,175 - +0.5 1,322,175 - +1.9 1,322,175 - -0.2 1,322,180 + -3.1 1,322,180 + +0.4 1,322,180 + -1.3 1,322,181 - -3.2 1,322,289 - +0.9 1,322,364 - -2.1 1,322,364 - +0.8 1,322,481 - bisC MCAODC_06545 0.12 +2.2 1,322,481 - bisC MCAODC_06545 0.12 +0.4 1,322,495 + bisC MCAODC_06545 0.13 -2.0 1,322,496 - bisC MCAODC_06545 0.13 +0.1 1,322,514 + bisC MCAODC_06545 0.13 +1.0 1,322,514 + bisC MCAODC_06545 0.13 +0.5 1,322,515 - bisC MCAODC_06545 0.13 +0.5 1,322,515 - bisC MCAODC_06545 0.13 -3.0 1,322,544 + bisC MCAODC_06545 0.15 -0.3 1,322,574 - bisC MCAODC_06545 0.16 +1.0 1,322,574 - bisC MCAODC_06545 0.16 +0.4 1,322,588 - bisC MCAODC_06545 0.17 +1.5 1,322,588 - bisC MCAODC_06545 0.17 +0.9 1,322,588 - bisC MCAODC_06545 0.17 -0.1 1,322,590 + bisC MCAODC_06545 0.17 -0.7 1,322,590 + bisC MCAODC_06545 0.17 -0.2 1,322,591 - bisC MCAODC_06545 0.17 -3.1 1,322,591 - bisC MCAODC_06545 0.17 +0.1 1,322,591 - bisC MCAODC_06545 0.17 +0.7 1,322,598 + bisC MCAODC_06545 0.17 +2.2 1,322,598 + bisC MCAODC_06545 0.17 -0.4 1,322,598 + bisC MCAODC_06545 0.17 +0.4 1,322,599 - bisC MCAODC_06545 0.17 -0.0 1,322,599 - bisC MCAODC_06545 0.17 +0.6 1,322,599 - bisC MCAODC_06545 0.17 -2.2 1,322,599 - bisC MCAODC_06545 0.17 +0.3 1,322,599 - bisC MCAODC_06545 0.17 +0.3 1,322,599 - bisC MCAODC_06545 0.17 +0.7 1,322,600 + bisC MCAODC_06545 0.17 -0.2 1,322,601 - bisC MCAODC_06545 0.17 +1.4 1,322,621 + bisC MCAODC_06545 0.18 -1.5 1,322,621 + bisC MCAODC_06545 0.18 -0.3 1,322,621 + bisC MCAODC_06545 0.18 +0.7 1,322,641 - bisC MCAODC_06545 0.19 +0.5 1,322,673 + bisC MCAODC_06545 0.20 +0.3 1,322,673 + bisC MCAODC_06545 0.20 +0.5 1,322,674 - bisC MCAODC_06545 0.20 +1.1 1,322,688 - bisC MCAODC_06545 0.21 +1.3 1,322,688 - bisC MCAODC_06545 0.21 +0.5 1,322,697 + bisC MCAODC_06545 0.21 -0.1 1,322,698 - bisC MCAODC_06545 0.21 +2.0 1,322,735 + bisC MCAODC_06545 0.23 -1.2 1,322,762 + bisC MCAODC_06545 0.24 +0.9 1,323,025 - bisC MCAODC_06545 0.35 +1.8 1,323,042 + bisC MCAODC_06545 0.36 -0.5 1,323,042 + bisC MCAODC_06545 0.36 +0.7 1,323,043 - bisC MCAODC_06545 0.36 -2.7 1,323,043 - bisC MCAODC_06545 0.36 -0.2 1,323,043 - bisC MCAODC_06545 0.36 -1.1 1,323,043 - bisC MCAODC_06545 0.36 +0.1 1,323,043 - bisC MCAODC_06545 0.36 +2.0
Or see this region's nucleotide sequence