Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdhE and MCAODC_04475 are separated by 329 nucleotides MCAODC_04475 and yiiE are separated by 216 nucleotides yiiE and MCAODC_04485 are separated by 693 nucleotides
MCAODC_04470: fdhE - formate dehydrogenase accessory protein FdhE, at 906,289 to 907,218
fdhE
MCAODC_04475: MCAODC_04475 - RHH-3 domain-containing protein, at 907,548 to 907,790
_04475
MCAODC_04480: yiiE - Uncharacterized protein YiiE, at 908,007 to 908,225
yiiE
MCAODC_04485: MCAODC_04485 - Transposase, at 908,919 to 909,833
_04485
Position (kb) 908
909 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 907.007 kb on - strand, within fdhE at 907.069 kb on + strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.139 kb on + strand at 907.139 kb on + strand at 907.168 kb on + strand at 907.217 kb on - strand at 907.256 kb on + strand at 907.266 kb on - strand at 907.266 kb on - strand at 907.290 kb on + strand at 907.291 kb on - strand at 907.314 kb on - strand at 907.361 kb on + strand at 907.362 kb on - strand at 907.373 kb on + strand at 907.388 kb on - strand at 907.388 kb on - strand at 907.421 kb on - strand at 907.421 kb on - strand at 907.569 kb on - strand at 907.569 kb on - strand at 907.569 kb on - strand at 907.569 kb on - strand at 907.647 kb on + strand, within MCAODC_04475 at 907.655 kb on - strand, within MCAODC_04475 at 907.655 kb on - strand, within MCAODC_04475 at 907.662 kb on + strand, within MCAODC_04475 at 907.663 kb on - strand, within MCAODC_04475 at 907.677 kb on + strand, within MCAODC_04475 at 907.677 kb on + strand, within MCAODC_04475 at 907.677 kb on + strand, within MCAODC_04475 at 907.678 kb on - strand, within MCAODC_04475 at 907.678 kb on - strand, within MCAODC_04475 at 907.755 kb on - strand, within MCAODC_04475 at 907.772 kb on + strand at 907.794 kb on + strand at 907.795 kb on - strand at 907.865 kb on + strand at 907.929 kb on - strand at 907.952 kb on - strand at 908.024 kb on + strand at 908.025 kb on - strand at 908.047 kb on - strand, within yiiE at 908.047 kb on - strand, within yiiE at 908.214 kb on - strand at 908.243 kb on + strand at 908.258 kb on + strand at 908.259 kb on - strand at 908.427 kb on - strand at 908.487 kb on - strand at 908.525 kb on - strand at 908.525 kb on - strand at 908.525 kb on - strand at 908.581 kb on - strand at 908.712 kb on + strand at 908.712 kb on + strand at 908.713 kb on - strand at 908.744 kb on + strand at 908.745 kb on - strand at 908.769 kb on + strand at 908.769 kb on + strand at 908.863 kb on - strand at 908.863 kb on - strand at 908.913 kb on + strand at 908.992 kb on - strand at 909.011 kb on + strand, within MCAODC_04485 at 909.113 kb on + strand, within MCAODC_04485
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 907,007 - fdhE MCAODC_04470 0.77 -2.0 907,069 + fdhE MCAODC_04470 0.84 -1.7 907,070 - fdhE MCAODC_04470 0.84 -0.5 907,070 - fdhE MCAODC_04470 0.84 +0.2 907,070 - fdhE MCAODC_04470 0.84 -2.2 907,070 - fdhE MCAODC_04470 0.84 +2.4 907,139 + +0.3 907,139 + -2.4 907,168 + -0.5 907,217 - -2.1 907,256 + -0.3 907,266 - -1.1 907,266 - -0.5 907,290 + +0.6 907,291 - -0.3 907,314 - +1.6 907,361 + -1.2 907,362 - -0.7 907,373 + +1.3 907,388 - +2.7 907,388 - +1.0 907,421 - -0.1 907,421 - +0.2 907,569 - +0.9 907,569 - -0.5 907,569 - -0.1 907,569 - -0.2 907,647 + MCAODC_04475 0.41 -2.6 907,655 - MCAODC_04475 0.44 +1.2 907,655 - MCAODC_04475 0.44 -0.2 907,662 + MCAODC_04475 0.47 +0.4 907,663 - MCAODC_04475 0.47 +0.3 907,677 + MCAODC_04475 0.53 -3.1 907,677 + MCAODC_04475 0.53 -0.1 907,677 + MCAODC_04475 0.53 -0.7 907,678 - MCAODC_04475 0.53 +2.0 907,678 - MCAODC_04475 0.53 +0.2 907,755 - MCAODC_04475 0.85 -1.7 907,772 + +1.8 907,794 + +1.2 907,795 - -4.5 907,865 + +2.5 907,929 - +2.1 907,952 - +0.8 908,024 + +0.5 908,025 - -2.1 908,047 - yiiE MCAODC_04480 0.18 +0.5 908,047 - yiiE MCAODC_04480 0.18 -1.6 908,214 - -0.2 908,243 + -0.2 908,258 + -2.0 908,259 - -1.8 908,427 - +1.2 908,487 - +0.8 908,525 - +1.9 908,525 - +0.3 908,525 - -0.5 908,581 - +0.7 908,712 + +0.5 908,712 + -0.2 908,713 - +1.1 908,744 + +1.3 908,745 - +0.9 908,769 + +1.6 908,769 + +1.4 908,863 - +0.4 908,863 - -1.6 908,913 + +1.5 908,992 - -2.8 909,011 + MCAODC_04485 0.10 -2.4 909,113 + MCAODC_04485 0.21 +0.6
Or see this region's nucleotide sequence